In vitro culture of tobacco protoplasts: Use of feeder techniques to support division of cells plated at low densities View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-12

AUTHORS

Dina Raveh, Eliezer Huberman, Esra Galun

ABSTRACT

Tobacco protoplasts obtained from leaf mesophyll cells and suspended in agar nutrient medium divided and produced colonies only when plated at high densities, above 104 cells per ml. At such densities coalescence of the expanding colonies occurred at high frequency. Nondividing X-irradiated protoplasts used as feeder cells supported division of viable protoplasts plated at densities as low as 102 cells per ml. The feeder cell technique should thus facilitate the application of screening procedures for the isolation of colonies originating from single mutated cells occurring in a suspended population. More... »

PAGES

216-222

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02618439

DOI

http://dx.doi.org/10.1007/bf02618439

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019345357

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4779005


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