Ontology type: schema:ScholarlyArticle
1979-06
AUTHORSHirofumi Uchimiya, S. G. Wildman
ABSTRACTThe nuclei and cytoplasm ofN. gossei andN. tabacum are compatible to the extent that reciprocal, interspecific F1 hybrids can be produced by conventional breeding techniques. Conditions were established in which manyN. gossei isolated chloroplasts could be seen by phase and fluorescence microscopy to adhere to 40% of the population of protoplasts obtained from white tissue of variegatedN. tabacum plants and to remain attached after washing the protoplasts. Chloroplasts also could be seen to enter the interior of the protoplasts. After treating albino protoplasts withN. gossei chloroplasts, the protoplasts were subjected to further conditions whereby 65 calluses containing shoots developed. TwentyN. tabacum protoplasts not treated with foreign chloroplasts also produced calluses with shoots to serve as a control. All calluses developed chlorophyll irrespective of whether or not the albino protoplasts had been treated with isolatedN. gossei chloroplasts. The Fraction 1 protein ofN. tabacum has a different electrophoretic mobility from the protein ofN. gossei or anN. gossei xN. tabacum F1 hybrid. The Fraction 1 protein large subunit is coded by chloroplast DNA, whereas the small subunit is coded by nuclear DNA. Fraction 1 protein was isolated from the variegated shoots of the 65 calluses obtained after treating albino protoplasts with foreign chloroplasts. Immunoelectrophoresis demonstrated the protein from each callus to have a mobility identical toN. tabacum protein. Therefore, under circumstances highly favorable for the direct transfer ofN. gossei isolated chloroplasts (and possibly nuclei also) intoN. tabacum protoplasts, no evidence was obtained to suggest that genetic information contained in the isolated foreign organelles was being translated into the polypeptides of either the large or small subunits of Fraction 1 protein contained in newly differentiated leaves derived from the protoplasts. More... »
PAGES463-468
http://scigraph.springernature.com/pub.10.1007/bf02618416
DOIhttp://dx.doi.org/10.1007/bf02618416
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1028604959
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of California Los Angeles",
"id": "https://www.grid.ac/institutes/grid.19006.3e",
"name": [
"Department of Biology, Molecular Biology Institute, University of California, 90024, Los Angeles, California"
],
"type": "Organization"
},
"familyName": "Uchimiya",
"givenName": "Hirofumi",
"id": "sg:person.01032535671.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032535671.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of California Los Angeles",
"id": "https://www.grid.ac/institutes/grid.19006.3e",
"name": [
"Department of Biology, Molecular Biology Institute, University of California, 90024, Los Angeles, California"
],
"type": "Organization"
},
"familyName": "Wildman",
"givenName": "S. G.",
"id": "sg:person.063033165.42",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.063033165.42"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1083/jcb.79.3.623",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000719292"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.69.8.2292",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001514157"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1104/pp.54.6.936",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009276107"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387314",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010233845",
"https://doi.org/10.1007/bf00387314"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387314",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010233845",
"https://doi.org/10.1007/bf00387314"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1104/pp.55.4.689",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014524424"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0044-328x(73)80103-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016664332"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023703429"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00388618",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024300197",
"https://doi.org/10.1007/bf00388618"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00388618",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024300197",
"https://doi.org/10.1007/bf00388618"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1266/jjg.50.235",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025382885"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00388272",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028670490",
"https://doi.org/10.1007/bf00388272"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00388272",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028670490",
"https://doi.org/10.1007/bf00388272"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.74.11.5109",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032655405"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2787(72)90115-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032678369"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2787(72)90115-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032678369"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269080",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044812453",
"https://doi.org/10.1007/bf00269080"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269080",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044812453",
"https://doi.org/10.1007/bf00269080"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0304-4211(79)90407-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046706811"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387313",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049595344",
"https://doi.org/10.1007/bf00387313"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387313",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049595344",
"https://doi.org/10.1007/bf00387313"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.74.8.3443",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050688407"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.127380",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062471445"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.187.4174.353",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062511016"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/oxfordjournals.jhered.a108685",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083485379"
],
"type": "CreativeWork"
}
],
"datePublished": "1979-06",
"datePublishedReg": "1979-06-01",
"description": "The nuclei and cytoplasm ofN. gossei andN. tabacum are compatible to the extent that reciprocal, interspecific F1 hybrids can be produced by conventional breeding techniques. Conditions were established in which manyN. gossei isolated chloroplasts could be seen by phase and fluorescence microscopy to adhere to 40% of the population of protoplasts obtained from white tissue of variegatedN. tabacum plants and to remain attached after washing the protoplasts. Chloroplasts also could be seen to enter the interior of the protoplasts. After treating albino protoplasts withN. gossei chloroplasts, the protoplasts were subjected to further conditions whereby 65 calluses containing shoots developed. TwentyN. tabacum protoplasts not treated with foreign chloroplasts also produced calluses with shoots to serve as a control. All calluses developed chlorophyll irrespective of whether or not the albino protoplasts had been treated with isolatedN. gossei chloroplasts. The Fraction 1 protein ofN. tabacum has a different electrophoretic mobility from the protein ofN. gossei or anN. gossei xN. tabacum F1 hybrid. The Fraction 1 protein large subunit is coded by chloroplast DNA, whereas the small subunit is coded by nuclear DNA. Fraction 1 protein was isolated from the variegated shoots of the 65 calluses obtained after treating albino protoplasts with foreign chloroplasts. Immunoelectrophoresis demonstrated the protein from each callus to have a mobility identical toN. tabacum protein. Therefore, under circumstances highly favorable for the direct transfer ofN. gossei isolated chloroplasts (and possibly nuclei also) intoN. tabacum protoplasts, no evidence was obtained to suggest that genetic information contained in the isolated foreign organelles was being translated into the polypeptides of either the large or small subunits of Fraction 1 protein contained in newly differentiated leaves derived from the protoplasts.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf02618416",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1312285",
"issn": [
"0883-8364"
],
"name": "In Vitro Cellular & Developmental Biology",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "15"
}
],
"name": "Nontranslation of foreign genetic information for fraction 1 protein under circumstances favorable for direct transfer ofNicotiana gossei isolated chloroplasts intoN. Tabacum protoplasts",
"pagination": "463-468",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"8843df1e2aaa1b894da5d63960b8b1abb03c4c6d745af6cac4f9c3572e665c43"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf02618416"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1028604959"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf02618416",
"https://app.dimensions.ai/details/publication/pub.1028604959"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:32",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46765_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2FBF02618416"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02618416'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02618416'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02618416'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02618416'
This table displays all metadata directly associated to this object as RDF triples.
129 TRIPLES
21 PREDICATES
46 URIs
19 LITERALS
7 BLANK NODES