HPLC analysis of phospholipids by evaporative laser light-scattering detection View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-05

AUTHORS

T. L. Mounts, S. L. Abidl, K. A. Rennick

ABSTRACT

High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary collumn chromatography for fatty acid composition, and by reverse-phase C-18 HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine. More... »

PAGES

438-442

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02540944

DOI

http://dx.doi.org/10.1007/bf02540944

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038317216


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