HPLC analysis of phospholipids by evaporative laser light-scattering detection View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-05

AUTHORS

T. L. Mounts, S. L. Abidl, K. A. Rennick

ABSTRACT

High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary collumn chromatography for fatty acid composition, and by reverse-phase C-18 HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine. More... »

PAGES

438-442

References to SciGraph publications

  • 1973-11. Chemical evaluation of oil from field-and storage-damaged soybeans in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1990-11. Effect of moisture, microwave heating, and live steam treatment on phospholipase D activity in soybeans and soy flakes in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1980. Lipid Enzymes: Lipases, Lipoxygenases, and “Hydroperoxidases” in AUTOXIDATION IN FOOD AND BIOLOGICAL SYSTEMS
  • 1990-01. The rhodium-catalyzed deuteration of unsaturated triglycerides in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1989-10. High performance liquid chromatography of phospholipids with flame ionization detection in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1979-10. Postharvest handling of soybeans: Effects on oil quality in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1990-11. HPLC analysis of phospholipids in crude oil for evaluation of soybean deterioration in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1981-10. Effect of degumming conditions on removal and quality of soybean lecithin in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02540944

    DOI

    http://dx.doi.org/10.1007/bf02540944

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1038317216


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