Assay of aflatoxin in peanuts and peanut products using acetone-hexane-water for extraction View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1965-06

AUTHORS

J. A. Robertson, Louise S. Lee, Alva F. Cucullu, L. A. Goldblatt

ABSTRACT

A quantitative method is described for the assay of aflatoxin in peanut products. The procedure involves extraction of aflatoxin from the sample with a homogeneous acetone-hexane-water solvent mixture followed by purification of the extract by phasic extraction of the aflatoxin with aqueous sodium chloride and then with chloroform. The purified chloroform extract is analyzed by thin-layer chromatography by comparison of the intensity of fluorescence of any aflatoxin with the intensity of a known standard. The aflatoxin analyses of peanuts were found to be very variable due to sampling, and this variability has been greatly reduced by finely grinding and thoroughly mixing 2 kg of the sample before removal of an aliquot for assay. The method is sensitive to approximately 2 parts per billion. More... »

PAGES

467-471

References to SciGraph publications

  • 1961-12. Toxicity associated with Certain Samples of Groundnuts in NATURE
  • 1964-08. Determination of the Aflatoxins in NATURE
  • 1963-06. Toxic Metabolites of Aspergillus Flavus in NATURE
  • 1961-01. Properties of cottonseed meals prepared with acetone-Petroleum ether-water azeotrope in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1965-06. The determination of aflatoxins in cottonseed products in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02540086

    DOI

    http://dx.doi.org/10.1007/bf02540086

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003456955

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14300201


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