Etude immunologique de serums de mouton antidiphteriques View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1960-12

AUTHORS

E. H. Relyveld, A. J. Van Triet, M. Raynaud, Made,pose;;e O. Bepoldin, Monsieur W. J. van Rhee

ABSTRACT

La préparation d'anticorps diphtériques neutralisants non précipitants à partir de sérums de mouton avec un rapport in vivo/in vitro élevé a été décrite. L'étude quantitative de la réaction toxine-antitoxine faite avec ces anticorps a montré que la neutralisation de l'antitoxine se fait suivant une règle simple. Il n'y a pas de formation de précipité, mais les anticorps peuvent être coprécipités par un système floculant. La mobilité électrophorètique des anticorps a été étudiée par l'électrophorèse en gélose et par l'immuno-électrophorèse. Les complexes “toxine-antitoxine non précipitant” ont une mobilité voisine de celle des beta-2-globulines de cheval. L'électrophorèse sur colonne de cellulose a montré que les anticorps précipitants et les anticorps neutralisants non précipitants ont la même mobilité électrophorètique. L'étude par double diffusion en gélose montre que les anticorps non précipitants radioactifs se fixent sur la ligne formée entre, d'une part, la toxine et d'autre part, les anticorps précipitants. Le titre floculant des sérums de rapport Lr/Lf faible, augmente si l'on y ajoute des anticorps neutralisants non précipitants, le Kf des préparations baisse d'abord pour remonter ensuite en fonction de la quantité d'anticorps non précipitants ajoutée. More... »

PAGES

349-379

References to SciGraph publications

  • 1959-12. Etude immunologique de serums de mouton antidiphteriques in ANTONIE VAN LEEUWENHOEK
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02539024

    DOI

    http://dx.doi.org/10.1007/bf02539024

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035371925

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/13740729


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