A rapid microozonolysis-GLC procedure for locating unsaturation in olefinic acids, including trienes and tetraenes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1969-03

AUTHORS

R. Kleiman, G. F. Spencer, F. R. Earle, I. A. Wolff

ABSTRACT

Increased versatility has been achieved in the identification of unknown olefinic fatty acids by ozonolysis. The method has been applied to purified methyl esters containing up to four double bonds. Aldehydic fragments, obtained from esters by the Stein-Nicolaides procedure (2), were determined by GLC on two columns of different polarity. Equivalent chain lengths of each fragment on the two columns provide identification. For monoenoic esters the location of the double bond is clearly indicated by the aldehyde and aldehyde-ester fragments. Dienes are identified by the aldehyde and aldehyde-ester fragments when the original chain length of the ester is known; the dialdehyde fragment provides confirmatory evidence. Trienes and tetraenes are analyzed by interrupting the ozonolysis at various times, thereby producing unsaturated, as well as saturated, aldehydes and aldehyde-esters. Unsaturated fragments locate the central or interior double bonds. More... »

PAGES

135-141

Journal

TITLE

Lipids

ISSUE

2

VOLUME

4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02531932

DOI

http://dx.doi.org/10.1007/bf02531932

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028138407


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