SyntheticcryIIIA gene fromBacillus thuringiensis improved for high expression in plants View Full Text


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Article Info

DATE

1992-09

AUTHORS

Dennis W. Sutton, Patti K. Havstad, John D. Kemp

ABSTRACT

A 1974 bp synthetic gene was constructed from chemically synthesized oligonucleotides in order to improve transgenic protein expression of thecryIIIA gene fromBacillus thuringiensis var.tenebrionis in transgenic tobacco.The crystal toxin genes (cry) fromB. thuringiensis are difficult to express in plants even when under the control of efficient plant regulatory sequences. We identified and eliminated five classes of sequence found throughout thecryIIIA gene that mimic eukaryotic processing signals and which may be responsible for the low levels of transcription and translation. Furthermore, the GC content of the gene was raised from 36% to 49% and the codon usage was changed to be more plant-like. When the synthetic gene was placed behind the cauliflower mosaic virus 35S promoter and the alfalfa mosaic virus translational enhancer, up to 0.6% of the total protein in transgenic tobacco plants wascryIIIA as measured from immunoblot analysis. Bioassay data using potato beetle larvae confirmed this estimate. More... »

PAGES

228-236

Journal

TITLE

Transgenic Research

ISSUE

5

VOLUME

1

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02524753

    DOI

    http://dx.doi.org/10.1007/bf02524753

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035402995

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1301214


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