Carbon-14 labelling of biomolecules induced by14CO ionized gas View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-03

AUTHORS

J. E. van Lier, L. Sanche

ABSTRACT

Ionized14CO gas provides a rapid method for producing14C-labelled biomolecules. The apparatus consists of a high vacuum system in which a small amount of14CO is ionized by electron impact. The resulting species drift towards a target where they interact with the molecule of interest to produce14C-labelled compounds. Since the reaction time is only 2 minutes, the method is particularly promising for producing tracer biomolecules with short-lived11C at high specific activities. We have studied the applicability of the method to various classes of compounds of biological importance, including sterids, alkaloids, prostaglandins, nucleosides, amino acids and proteins. All compounds treated gave rise to14C addition and degradation products. Furthermore, for some compounds, chromatographic analysis in multiple systems followed by derivatization and crystallization to constant specific activity, indicated that carbon exchange may occur to produce the labelled, but otherwise unaltered substrate in yields of the order of 10–100 mCi/mol. More conclusive proof of radiochemical identity must await production of larger quantities of material and rigorous purification including at least two different chromatographic techniques. More... »

PAGES

273-290

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02517926

DOI

http://dx.doi.org/10.1007/bf02517926

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028605200


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