Proliferation and differentiation of cultured human follicular keratinocytes are not influenced by biotin View Full Text


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Article Info

DATE

1996-01

AUTHORS

Alain Limat, Terttu Suormala, Thomas Hunziker, Ernst R. Waelti, Lasse R. Braathen, Regula Baumgartner

ABSTRACT

In humans and in animals, biotin deficiency causes pathological changes in the skin and its appendages. High doses of biotin may also have beneficial effects on skin, hair and fingernails in humans and animals with normal biotin status. Therefore, we investigated the effects of low and high concentrations of biotin on proliferation and differentiation of cultured outer root sheath cells from human hair follicles as an in vitro model for skin. The activities of biotin-dependent carboxylases were measured to evaluate the biotin status of the cells. In monolayer cultures of outer root sheath cells, proliferation and expression of the differentiation-specific keratins K1 and K10 were not influenced by extremely low concentrations of biotin (<2 x 10(-10) mol/l) or by pharmacological doses of biotin (10(-5) mol/l). Biotin deficiency of the cells was confirmed under the former condition by demonstrating decreased activities of the mitochondrial carboxylases. In organotypic cocultures of outer root sheath cells and dermal fibroblasts, in which stratified epithelia resembling epidermis were developed, the biotin concentration had no effect on the expression of all tested epidermal differentiation markers, including the suprabasal keratins K1 and K10, the hyperproliferation-associated keratin K16, involucrin and filaggrin. More... »

PAGES

31-38

References to SciGraph publications

  • 1983-07. Keratin Classes: Molecular Markers for Different Types of Epithelial Differentiation in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1987-06. Effect of growth environment on spatial expression of involucrin by human epidermal keratinocytes in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1986-10. Serial Cultivation of Single Keratinocytes from the Outer Root Sheath of Human Scalp Hair Follicles in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1993-10. Effects of 1α,25-dihydroxy-vitamin D3 and calcipotriol on organotypic cultures of outer root sheath cells: a potential model to evaluate antipsoriatic drugs in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1993-11. Upper Human Hair Follicle Contains a Subpopulation of Keratinocytes with Superior In Vitro Proliferative Potential in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1991-10. Keratin 17: a useful marker in anti-psoriatic therapies in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1988. Intermediate Filament Associated Protein of Epidermis in THE BIOLOGY OF WOOL AND HAIR
  • 1990-01. Comparison of patients with complete and partial biotinidase deficiency: Biochemical studies in JOURNAL OF INHERITED METABOLIC DISEASE
  • 1990-07. Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1983-07. Involucrin and Other Markers of Keratinocyte Terminal Differentiation in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1989-05. Post-Mitotic Human Dermal Fibroblasts Efficiently Support the Growth of Human Follicular Keratinocytes in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1955-09. Responses of the Superficial Portion of the Human Pilosebaceous Apparatus to Controlled Injury1 in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02505040

    DOI

    http://dx.doi.org/10.1007/bf02505040

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007886478

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8750932


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