Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression inEscherichia ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-06

AUTHORS

Rüdiger Schulz, Klaus Steinmüller, Manfred Klaas, Christoph Forreiter, Sören Rasmussen, Claudia Hiller, Klaus Apel

ABSTRACT

The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light. More... »

PAGES

355-361

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02464904

DOI

http://dx.doi.org/10.1007/bf02464904

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011555173

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2671659


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N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02464904'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02464904'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02464904'


 

This table displays all metadata directly associated to this object as RDF triples.

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