Effects of staurosporine on Ca2+-dependent and Ca2+-independent [14C]GABA release from rat brain synaptosomes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-07

AUTHORS

L. G. Storchak, N. G. Pozdnyakova, N. H. Himmelreich

ABSTRACT

The effect of a protein kinase inhibitor, staurosporine, on Ca2+-dependent and Ca2+-independent release of [14C]GABA in isolated rat brain synaptosomes was studied. Calcium-dependent [14C]GABA release was stimulated by depolarization with a K+ channel blocker, 4-aminopyridine (4-AP), or high K+ concentration. It has been shown that the effect of 4-AP is Ca2+-dependent, while high K+ is able to evoke [14C]GABA release in both Ca2+-dependent and Ca2+-independent manners. In addition, Ca2+-independent [14C]GABA release was studied using α-latrotoxin (LTX) as a tool. Pretreatment of synaptosomes with staurosporine resulted in pronounced inhibition of 4-AP-stimulated Ca2+-dependent [14C]GABA release. The inhibitory effect of staurosporine on [14C]GABA release was not due to modulation of 4-AP-promoted45Ca2+ influx into synaptosomes. If the process of [14C]GABA release occurred in the Ca2+-independent manner irrespectively of what, LTX or high K+, stimulated this process, it was not inhibited by staurosporine. Considering the above findings, it is reasonable to assume that the absence of Ca2+ in the extracellular medium created conditions for activation of the process of neurotransmitter release without Ca2+-dependent dephosphorylation of neuronal phosphoproteins; as a consequence, regulation of exocytotic process was modulated in such a manner that inhibition of protein kinases did not disturb exocytosis. More... »

PAGES

278-282

References to SciGraph publications

Journal

TITLE

Neurophysiology

ISSUE

4-5

VOLUME

29

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02461242

DOI

http://dx.doi.org/10.1007/bf02461242

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035713825


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Storchak", 
        "givenName": "L. G.", 
        "id": "sg:person.015254252667.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015254252667.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pozdnyakova", 
        "givenName": "N. G.", 
        "id": "sg:person.01277076463.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277076463.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Himmelreich", 
        "givenName": "N. H.", 
        "id": "sg:person.01016527735.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016527735.99"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/326704a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004402623", 
          "https://doi.org/10.1038/326704a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0306-4522(93)90366-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005409719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0306-4522(93)90366-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005409719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(94)00790-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005531793"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00169064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006538056", 
          "https://doi.org/10.1007/bf00169064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00169064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006538056", 
          "https://doi.org/10.1007/bf00169064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(92)90072-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010685926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.1978.tb12406.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012143028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.1992.tb09736.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030085855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(74)31050-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030573040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(94)90353-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033806078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.110.2.449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040928164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(95)00084-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053045239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8430330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077314659", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078833699", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078838317", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082397280", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.15-12-08246.1995", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082880131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.16-19-05905.1996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082963652"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-07", 
    "datePublishedReg": "1997-07-01", 
    "description": "The effect of a protein kinase inhibitor, staurosporine, on Ca2+-dependent and Ca2+-independent release of [14C]GABA in isolated rat brain synaptosomes was studied. Calcium-dependent [14C]GABA release was stimulated by depolarization with a K+ channel blocker, 4-aminopyridine (4-AP), or high K+ concentration. It has been shown that the effect of 4-AP is Ca2+-dependent, while high K+ is able to evoke [14C]GABA release in both Ca2+-dependent and Ca2+-independent manners. In addition, Ca2+-independent [14C]GABA release was studied using \u03b1-latrotoxin (LTX) as a tool. Pretreatment of synaptosomes with staurosporine resulted in pronounced inhibition of 4-AP-stimulated Ca2+-dependent [14C]GABA release. The inhibitory effect of staurosporine on [14C]GABA release was not due to modulation of 4-AP-promoted45Ca2+ influx into synaptosomes. If the process of [14C]GABA release occurred in the Ca2+-independent manner irrespectively of what, LTX or high K+, stimulated this process, it was not inhibited by staurosporine. Considering the above findings, it is reasonable to assume that the absence of Ca2+ in the extracellular medium created conditions for activation of the process of neurotransmitter release without Ca2+-dependent dephosphorylation of neuronal phosphoproteins; as a consequence, regulation of exocytotic process was modulated in such a manner that inhibition of protein kinases did not disturb exocytosis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02461242", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1016854", 
        "issn": [
          "0090-2977", 
          "1573-9007"
        ], 
        "name": "Neurophysiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4-5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "29"
      }
    ], 
    "name": "Effects of staurosporine on Ca2+-dependent and Ca2+-independent [14C]GABA release from rat brain synaptosomes", 
    "pagination": "278-282", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "aa20b0d02069b1ac2080c45aa8f2b15241e33ce36dbb99678586856d4f7536b2"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02461242"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035713825"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02461242", 
      "https://app.dimensions.ai/details/publication/pub.1035713825"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46754_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02461242"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02461242'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02461242'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02461242'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02461242'


 

This table displays all metadata directly associated to this object as RDF triples.

127 TRIPLES      21 PREDICATES      45 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02461242 schema:about anzsrc-for:11
2 anzsrc-for:1109
3 schema:author Nf31cad67287b4152b4157fde00fa34ff
4 schema:citation sg:pub.10.1007/bf00169064
5 sg:pub.10.1038/326704a0
6 https://app.dimensions.ai/details/publication/pub.1077314659
7 https://app.dimensions.ai/details/publication/pub.1078833699
8 https://app.dimensions.ai/details/publication/pub.1078838317
9 https://app.dimensions.ai/details/publication/pub.1082397280
10 https://doi.org/10.1016/0014-5793(94)00790-x
11 https://doi.org/10.1016/0041-0101(95)00084-y
12 https://doi.org/10.1016/0076-6879(74)31050-6
13 https://doi.org/10.1016/0306-4522(93)90366-n
14 https://doi.org/10.1016/0896-6273(92)90072-l
15 https://doi.org/10.1016/0896-6273(94)90353-0
16 https://doi.org/10.1083/jcb.110.2.449
17 https://doi.org/10.1111/j.1471-4159.1978.tb12406.x
18 https://doi.org/10.1111/j.1471-4159.1992.tb09736.x
19 https://doi.org/10.1126/science.8430330
20 https://doi.org/10.1523/jneurosci.15-12-08246.1995
21 https://doi.org/10.1523/jneurosci.16-19-05905.1996
22 schema:datePublished 1997-07
23 schema:datePublishedReg 1997-07-01
24 schema:description The effect of a protein kinase inhibitor, staurosporine, on Ca2+-dependent and Ca2+-independent release of [14C]GABA in isolated rat brain synaptosomes was studied. Calcium-dependent [14C]GABA release was stimulated by depolarization with a K+ channel blocker, 4-aminopyridine (4-AP), or high K+ concentration. It has been shown that the effect of 4-AP is Ca2+-dependent, while high K+ is able to evoke [14C]GABA release in both Ca2+-dependent and Ca2+-independent manners. In addition, Ca2+-independent [14C]GABA release was studied using α-latrotoxin (LTX) as a tool. Pretreatment of synaptosomes with staurosporine resulted in pronounced inhibition of 4-AP-stimulated Ca2+-dependent [14C]GABA release. The inhibitory effect of staurosporine on [14C]GABA release was not due to modulation of 4-AP-promoted45Ca2+ influx into synaptosomes. If the process of [14C]GABA release occurred in the Ca2+-independent manner irrespectively of what, LTX or high K+, stimulated this process, it was not inhibited by staurosporine. Considering the above findings, it is reasonable to assume that the absence of Ca2+ in the extracellular medium created conditions for activation of the process of neurotransmitter release without Ca2+-dependent dephosphorylation of neuronal phosphoproteins; as a consequence, regulation of exocytotic process was modulated in such a manner that inhibition of protein kinases did not disturb exocytosis.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N02ee7b36887f4d348e514ff1dbbc234b
29 N17f5ac865ac84fcfa9d23acd293572ea
30 sg:journal.1016854
31 schema:name Effects of staurosporine on Ca2+-dependent and Ca2+-independent [14C]GABA release from rat brain synaptosomes
32 schema:pagination 278-282
33 schema:productId N255252ebb75643d1abfed1b4ba83d317
34 N52c48c3d476a4670b8c4bb84f8d58992
35 N61551b6442c5492ebd7b6891b506af64
36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035713825
37 https://doi.org/10.1007/bf02461242
38 schema:sdDatePublished 2019-04-11T13:30
39 schema:sdLicense https://scigraph.springernature.com/explorer/license/
40 schema:sdPublisher Na8626affc9174418a7ed6ee5cf80a103
41 schema:url http://link.springer.com/10.1007/BF02461242
42 sgo:license sg:explorer/license/
43 sgo:sdDataset articles
44 rdf:type schema:ScholarlyArticle
45 N02ee7b36887f4d348e514ff1dbbc234b schema:volumeNumber 29
46 rdf:type schema:PublicationVolume
47 N17f5ac865ac84fcfa9d23acd293572ea schema:issueNumber 4-5
48 rdf:type schema:PublicationIssue
49 N255252ebb75643d1abfed1b4ba83d317 schema:name dimensions_id
50 schema:value pub.1035713825
51 rdf:type schema:PropertyValue
52 N3e511fc09db246d487c84f6148b61d54 rdf:first sg:person.01016527735.99
53 rdf:rest rdf:nil
54 N52c48c3d476a4670b8c4bb84f8d58992 schema:name readcube_id
55 schema:value aa20b0d02069b1ac2080c45aa8f2b15241e33ce36dbb99678586856d4f7536b2
56 rdf:type schema:PropertyValue
57 N61551b6442c5492ebd7b6891b506af64 schema:name doi
58 schema:value 10.1007/bf02461242
59 rdf:type schema:PropertyValue
60 Na8626affc9174418a7ed6ee5cf80a103 schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 Ndcd176890b6744748ca7cda2910158ce rdf:first sg:person.01277076463.41
63 rdf:rest N3e511fc09db246d487c84f6148b61d54
64 Nf31cad67287b4152b4157fde00fa34ff rdf:first sg:person.015254252667.00
65 rdf:rest Ndcd176890b6744748ca7cda2910158ce
66 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
67 schema:name Medical and Health Sciences
68 rdf:type schema:DefinedTerm
69 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
70 schema:name Neurosciences
71 rdf:type schema:DefinedTerm
72 sg:journal.1016854 schema:issn 0090-2977
73 1573-9007
74 schema:name Neurophysiology
75 rdf:type schema:Periodical
76 sg:person.01016527735.99 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
77 schema:familyName Himmelreich
78 schema:givenName N. H.
79 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016527735.99
80 rdf:type schema:Person
81 sg:person.01277076463.41 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
82 schema:familyName Pozdnyakova
83 schema:givenName N. G.
84 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277076463.41
85 rdf:type schema:Person
86 sg:person.015254252667.00 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
87 schema:familyName Storchak
88 schema:givenName L. G.
89 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015254252667.00
90 rdf:type schema:Person
91 sg:pub.10.1007/bf00169064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006538056
92 https://doi.org/10.1007/bf00169064
93 rdf:type schema:CreativeWork
94 sg:pub.10.1038/326704a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004402623
95 https://doi.org/10.1038/326704a0
96 rdf:type schema:CreativeWork
97 https://app.dimensions.ai/details/publication/pub.1077314659 schema:CreativeWork
98 https://app.dimensions.ai/details/publication/pub.1078833699 schema:CreativeWork
99 https://app.dimensions.ai/details/publication/pub.1078838317 schema:CreativeWork
100 https://app.dimensions.ai/details/publication/pub.1082397280 schema:CreativeWork
101 https://doi.org/10.1016/0014-5793(94)00790-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005531793
102 rdf:type schema:CreativeWork
103 https://doi.org/10.1016/0041-0101(95)00084-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1053045239
104 rdf:type schema:CreativeWork
105 https://doi.org/10.1016/0076-6879(74)31050-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030573040
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1016/0306-4522(93)90366-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1005409719
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/0896-6273(92)90072-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1010685926
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/0896-6273(94)90353-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033806078
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1083/jcb.110.2.449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040928164
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1111/j.1471-4159.1978.tb12406.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012143028
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1111/j.1471-4159.1992.tb09736.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030085855
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1126/science.8430330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655570
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1523/jneurosci.15-12-08246.1995 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082880131
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1523/jneurosci.16-19-05905.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082963652
124 rdf:type schema:CreativeWork
125 https://www.grid.ac/institutes/grid.419966.5 schema:alternateName Palladin Institute of Biochemistry
126 schema:name Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine
127 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...