Control of dissolved oxygen concentration in mammalian cell culture using a computer-coupled mass flow controller View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-09

AUTHORS

Heung C. Jung, Deok K. Oh, Jung H. Kim

ABSTRACT

A simple proportional control system for dissolved oxygen (DO) concentration in cell culture medium was developed by using a computer-coupled mass flow controller. The DO levels were very stable during the cultivation of Vero-6, while flow rates of air and/or oxygen enriched air were gradually changed depending on the DO concentration and the preset DO level. Vero-6 cells could grow normally to the confluence in the range of 30% and 50% of DO. Growth of Vero-6 at 10% of DO was markedly retarded. More... »

PAGES

405-408

References to SciGraph publications

  • 1981-12. Oxygen demand and supply in cell culture in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02447478

    DOI

    http://dx.doi.org/10.1007/bf02447478

    DIMENSIONS

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