Immobilization and culture of coffee (Coffeaarabica L.) cells on novel culture systems using a basket-shaped unit, “EGSTAR” View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-07

AUTHORS

Kenji Koge, Yutaka Orihara, Tsutomu Furuya

ABSTRACT

A simple and new basket-shaped unit for agitation made of stainless steel (EGSTAR), in which immobilized coffee cells in Ca-alginate gel beads were packed, was placed in a jar fermentor (System-1). This system allowed the plant cells to grow submersed in the unit even at high agitation speed (650 rpm). Only a small amount of cells existed out of the EGSTAR. Most of the purine alkaloids produced were released into the medium. Suspended coffee cells in the jar fermentor were also possibly immobilized onto a polyurethane foam sheet fixed inside the net of the EGSTAR (System-2). The total cells in System-2 biotransformed theobromine to caffeine (77.9%). Other plant cell suspensions were also immobilized as efficiently as were the coffee cells in this system. Thus, System-2 is a simple and convenient system for immobilization of plant cells to produce secondary metabolites. More... »

PAGES

313-318

Journal

TITLE

Biotechnology Letters

ISSUE

4

VOLUME

6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02439318

DOI

http://dx.doi.org/10.1007/bf02439318

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012346502


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