T-DNA structure in transgenic tobacco plants with multiple independent integration sites View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-10

AUTHORS

Albert Spielmann, Robert B. Simpson

ABSTRACT

Transgenic tobacco plants were produced by inoculation of leaf disks withAgrobacterium tumefaciens harboring a disarmed binary vector containing soybean leghemoglobin Lbc3 and glycinin G2 genes. Physical and genetic characterization of these plants indicated that one to six copies of DNA from the vector were transferred and maintained in the plant genome. Approximately 30% of the copies transferred were found to be incomplete or rearranged and in some cases joined as inverted repeats. The transferred DNA was found at multiple genetic loci in five of the six cases examined. In one plant, kanamycin-resistance traits were at four independent chromosomal positions, although two were genetically linked at about 3 centimorgans. Thus,Agrobacterium-mediated DNA transfer to plants has some characteristics in common with “natural” systems in animals, such as retroviral or P-element derived systems, some characteristics in common with “artificial” systems, such as microinjection, electroporation, or calcium phosphate coprecipitation techniques, and some novel characteristics. More... »

PAGES

34-41

References to SciGraph publications

  • 1986-01. Two unlinked T-DNAs can transform the same tobacco plant cell and segregate in the F1 generation in MOLECULAR GENETICS AND GENOMICS
  • 1983-06. Nopaline T-DNA is maintained during regeneration and generative propagation of transformed tobacco plants in MOLECULAR GENETICS AND GENOMICS
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1985-05. Totipotency of tomato protoplasts in THEORETICAL AND APPLIED GENETICS
  • 1986-11. A disarmed binary vector from Agrobacterium tumefaciens functions in Agrobacterium rhizogenes in PLANT MOLECULAR BIOLOGY
  • 1981-10. Mendelian transcmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1985-07. Transformation of plant protoplasts with DNA: cotransformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences in PLANT MOLECULAR BIOLOGY
  • 1982-08. De novo methylation and expression of retroviral genomes during mouse embryogenesis in NATURE
  • 1985. DNA Flux Across Genetic Barriers: The Crown Gall Phenomenon in GENETIC FLUX IN PLANTS
  • 1986-03. Independent integration and seed-transmission of the TR-DNA of the octopine Ti plasmid pTi Ach5 in Nicotiana plumbaginifolia in PLANT MOLECULAR BIOLOGY
  • 1985-12. Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interaction in MOLECULAR GENETICS AND GENOMICS
  • 1984-05. Sexual transmission of T-DNA in abnormal tobacco regenerants transformed by octopine and nopaline strains of Agrobacterium tumefaciens in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02428030

    DOI

    http://dx.doi.org/10.1007/bf02428030

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026692189


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Molecular Biology Group, ARCO Plant Cell Research Institute, 6560 Trinity Ct., 94568, Dublin, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Spielmann", 
            "givenName": "Albert", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Molecular Biology Group, ARCO Plant Cell Research Institute, 6560 Trinity Ct., 94568, Dublin, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Simpson", 
            "givenName": "Robert B.", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000852526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000852526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/13.2.459", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004658910"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00425565", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008020599", 
              "https://doi.org/10.1007/bf00425565"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(81)90176-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009056250"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.81.12.3825", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010484001"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330254", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011873691", 
              "https://doi.org/10.1007/bf00330254"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.79.20.6322", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015877276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1965.tb06874.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016020981"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016124491", 
              "https://doi.org/10.1007/bf00027303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016124491", 
              "https://doi.org/10.1007/bf00027303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(80)80049-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016541918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/303179a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018421874", 
              "https://doi.org/10.1038/303179a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019927271", 
              "https://doi.org/10.1007/bf00027133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019927271", 
              "https://doi.org/10.1007/bf00027133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(80)90178-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022719309"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(80)90095-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024871846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(81)90143-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026467580"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/298623a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026714891", 
              "https://doi.org/10.1038/298623a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00020641", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026807225", 
              "https://doi.org/10.1007/bf00020641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00020641", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026807225", 
              "https://doi.org/10.1007/bf00020641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331342", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027029857", 
              "https://doi.org/10.1007/bf00331342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.77.12.7347", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027142782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.10.3320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029081242"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.pp.35.060184.002131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030599579"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00662431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033394583", 
              "https://doi.org/10.1007/bf00662431"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00662431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033394583", 
              "https://doi.org/10.1007/bf00662431"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036019563", 
              "https://doi.org/10.1007/bf00330255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(82)90464-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036096412"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(81)90343-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036391311"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.81.24.8014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037339771"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.79.13.4055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039201422"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-7091-8765-4_2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039298445", 
              "https://doi.org/10.1007/978-3-7091-8765-4_2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(82)90181-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043719981"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331085", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044783076", 
              "https://doi.org/10.1007/bf00331085"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270619", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045968668", 
              "https://doi.org/10.1007/bf00270619"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270619", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045968668", 
              "https://doi.org/10.1007/bf00270619"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330528", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046206037", 
              "https://doi.org/10.1007/bf00330528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.78.7.4151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046272833"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.223.4635.496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062528157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.227.4691.1229", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062529900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.6251546", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062635866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.6289436", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062636078"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02032.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077183680"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02201.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077183716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03996.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1080025490"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1986-10", 
        "datePublishedReg": "1986-10-01", 
        "description": "Transgenic tobacco plants were produced by inoculation of leaf disks withAgrobacterium tumefaciens harboring a disarmed binary vector containing soybean leghemoglobin Lbc3 and glycinin G2 genes. Physical and genetic characterization of these plants indicated that one to six copies of DNA from the vector were transferred and maintained in the plant genome. Approximately 30% of the copies transferred were found to be incomplete or rearranged and in some cases joined as inverted repeats. The transferred DNA was found at multiple genetic loci in five of the six cases examined. In one plant, kanamycin-resistance traits were at four independent chromosomal positions, although two were genetically linked at about 3 centimorgans. Thus,Agrobacterium-mediated DNA transfer to plants has some characteristics in common with \u201cnatural\u201d systems in animals, such as retroviral or P-element derived systems, some characteristics in common with \u201cartificial\u201d systems, such as microinjection, electroporation, or calcium phosphate coprecipitation techniques, and some novel characteristics.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf02428030", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297380", 
            "issn": [
              "1617-4615", 
              "1432-1874"
            ], 
            "name": "Molecular Genetics and Genomics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "205"
          }
        ], 
        "name": "T-DNA structure in transgenic tobacco plants with multiple independent integration sites", 
        "pagination": "34-41", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "7f28d799d1af5a82af78c7700102cc421377669921fc114f5963ba081e7e9b3f"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf02428030"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1026692189"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf02428030", 
          "https://app.dimensions.ai/details/publication/pub.1026692189"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:30", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46751_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF02428030"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02428030'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02428030'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02428030'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02428030'


     

    This table displays all metadata directly associated to this object as RDF triples.

    207 TRIPLES      21 PREDICATES      69 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf02428030 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N415bc2bcab6d40e2819940840f3c6392
    4 schema:citation sg:pub.10.1007/978-3-7091-8765-4_2
    5 sg:pub.10.1007/bf00020641
    6 sg:pub.10.1007/bf00027133
    7 sg:pub.10.1007/bf00027303
    8 sg:pub.10.1007/bf00270619
    9 sg:pub.10.1007/bf00330254
    10 sg:pub.10.1007/bf00330255
    11 sg:pub.10.1007/bf00330528
    12 sg:pub.10.1007/bf00331085
    13 sg:pub.10.1007/bf00331342
    14 sg:pub.10.1007/bf00425565
    15 sg:pub.10.1007/bf00662431
    16 sg:pub.10.1038/298623a0
    17 sg:pub.10.1038/303179a0
    18 https://doi.org/10.1002/j.1460-2075.1984.tb02032.x
    19 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x
    20 https://doi.org/10.1002/j.1460-2075.1985.tb03996.x
    21 https://doi.org/10.1016/0022-2836(80)90095-9
    22 https://doi.org/10.1016/0092-8674(80)90178-6
    23 https://doi.org/10.1016/0092-8674(81)90176-8
    24 https://doi.org/10.1016/0092-8674(81)90343-3
    25 https://doi.org/10.1016/0092-8674(82)90181-7
    26 https://doi.org/10.1016/0092-8674(82)90464-0
    27 https://doi.org/10.1016/0378-1119(81)90143-8
    28 https://doi.org/10.1016/0378-1119(84)90122-7
    29 https://doi.org/10.1016/s0022-2836(75)80083-0
    30 https://doi.org/10.1016/s0092-8674(80)80049-3
    31 https://doi.org/10.1073/pnas.77.12.7347
    32 https://doi.org/10.1073/pnas.78.7.4151
    33 https://doi.org/10.1073/pnas.79.13.4055
    34 https://doi.org/10.1073/pnas.79.20.6322
    35 https://doi.org/10.1073/pnas.81.12.3825
    36 https://doi.org/10.1073/pnas.81.24.8014
    37 https://doi.org/10.1073/pnas.82.10.3320
    38 https://doi.org/10.1093/nar/13.2.459
    39 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    40 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
    41 https://doi.org/10.1126/science.223.4635.496
    42 https://doi.org/10.1126/science.227.4691.1229
    43 https://doi.org/10.1126/science.6251546
    44 https://doi.org/10.1126/science.6289436
    45 https://doi.org/10.1146/annurev.pp.35.060184.002131
    46 schema:datePublished 1986-10
    47 schema:datePublishedReg 1986-10-01
    48 schema:description Transgenic tobacco plants were produced by inoculation of leaf disks withAgrobacterium tumefaciens harboring a disarmed binary vector containing soybean leghemoglobin Lbc3 and glycinin G2 genes. Physical and genetic characterization of these plants indicated that one to six copies of DNA from the vector were transferred and maintained in the plant genome. Approximately 30% of the copies transferred were found to be incomplete or rearranged and in some cases joined as inverted repeats. The transferred DNA was found at multiple genetic loci in five of the six cases examined. In one plant, kanamycin-resistance traits were at four independent chromosomal positions, although two were genetically linked at about 3 centimorgans. Thus,Agrobacterium-mediated DNA transfer to plants has some characteristics in common with “natural” systems in animals, such as retroviral or P-element derived systems, some characteristics in common with “artificial” systems, such as microinjection, electroporation, or calcium phosphate coprecipitation techniques, and some novel characteristics.
    49 schema:genre research_article
    50 schema:inLanguage en
    51 schema:isAccessibleForFree false
    52 schema:isPartOf N6b1b7c83267d46af80960774eb2c9a97
    53 Nb7c90d42143b48d4ad6114c215a93586
    54 sg:journal.1297380
    55 schema:name T-DNA structure in transgenic tobacco plants with multiple independent integration sites
    56 schema:pagination 34-41
    57 schema:productId N34f14b9a54714a418990f22277e5db54
    58 N39474e9fc9ba4e9e808da6549dcb6ef6
    59 N9a5ffb3f895b4c9cbe195cb9bb49528e
    60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026692189
    61 https://doi.org/10.1007/bf02428030
    62 schema:sdDatePublished 2019-04-11T13:30
    63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    64 schema:sdPublisher N4d125ba0720745ffab40c819aa87fe9b
    65 schema:url http://link.springer.com/10.1007/BF02428030
    66 sgo:license sg:explorer/license/
    67 sgo:sdDataset articles
    68 rdf:type schema:ScholarlyArticle
    69 N34f14b9a54714a418990f22277e5db54 schema:name dimensions_id
    70 schema:value pub.1026692189
    71 rdf:type schema:PropertyValue
    72 N39474e9fc9ba4e9e808da6549dcb6ef6 schema:name readcube_id
    73 schema:value 7f28d799d1af5a82af78c7700102cc421377669921fc114f5963ba081e7e9b3f
    74 rdf:type schema:PropertyValue
    75 N40cd8f09a8cf49dd8c3922979dca774d schema:name Molecular Biology Group, ARCO Plant Cell Research Institute, 6560 Trinity Ct., 94568, Dublin, CA, USA
    76 rdf:type schema:Organization
    77 N415bc2bcab6d40e2819940840f3c6392 rdf:first Ne8656d2d9f24441e88e25e2ac8b0eb1f
    78 rdf:rest N9540090df03243449451246f97f0651d
    79 N4d125ba0720745ffab40c819aa87fe9b schema:name Springer Nature - SN SciGraph project
    80 rdf:type schema:Organization
    81 N6b1b7c83267d46af80960774eb2c9a97 schema:volumeNumber 205
    82 rdf:type schema:PublicationVolume
    83 N8e88077421c44ebaaac397be5cde9295 schema:name Molecular Biology Group, ARCO Plant Cell Research Institute, 6560 Trinity Ct., 94568, Dublin, CA, USA
    84 rdf:type schema:Organization
    85 N9540090df03243449451246f97f0651d rdf:first Nfa7545861917454bb18fe1c8e862ef8f
    86 rdf:rest rdf:nil
    87 N9a5ffb3f895b4c9cbe195cb9bb49528e schema:name doi
    88 schema:value 10.1007/bf02428030
    89 rdf:type schema:PropertyValue
    90 Nb7c90d42143b48d4ad6114c215a93586 schema:issueNumber 1
    91 rdf:type schema:PublicationIssue
    92 Ne8656d2d9f24441e88e25e2ac8b0eb1f schema:affiliation N40cd8f09a8cf49dd8c3922979dca774d
    93 schema:familyName Spielmann
    94 schema:givenName Albert
    95 rdf:type schema:Person
    96 Nfa7545861917454bb18fe1c8e862ef8f schema:affiliation N8e88077421c44ebaaac397be5cde9295
    97 schema:familyName Simpson
    98 schema:givenName Robert B.
    99 rdf:type schema:Person
    100 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    101 schema:name Biological Sciences
    102 rdf:type schema:DefinedTerm
    103 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    104 schema:name Genetics
    105 rdf:type schema:DefinedTerm
    106 sg:journal.1297380 schema:issn 1432-1874
    107 1617-4615
    108 schema:name Molecular Genetics and Genomics
    109 rdf:type schema:Periodical
    110 sg:pub.10.1007/978-3-7091-8765-4_2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039298445
    111 https://doi.org/10.1007/978-3-7091-8765-4_2
    112 rdf:type schema:CreativeWork
    113 sg:pub.10.1007/bf00020641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026807225
    114 https://doi.org/10.1007/bf00020641
    115 rdf:type schema:CreativeWork
    116 sg:pub.10.1007/bf00027133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019927271
    117 https://doi.org/10.1007/bf00027133
    118 rdf:type schema:CreativeWork
    119 sg:pub.10.1007/bf00027303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016124491
    120 https://doi.org/10.1007/bf00027303
    121 rdf:type schema:CreativeWork
    122 sg:pub.10.1007/bf00270619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045968668
    123 https://doi.org/10.1007/bf00270619
    124 rdf:type schema:CreativeWork
    125 sg:pub.10.1007/bf00330254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011873691
    126 https://doi.org/10.1007/bf00330254
    127 rdf:type schema:CreativeWork
    128 sg:pub.10.1007/bf00330255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036019563
    129 https://doi.org/10.1007/bf00330255
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/bf00330528 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046206037
    132 https://doi.org/10.1007/bf00330528
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/bf00331085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044783076
    135 https://doi.org/10.1007/bf00331085
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/bf00331342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027029857
    138 https://doi.org/10.1007/bf00331342
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1007/bf00425565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008020599
    141 https://doi.org/10.1007/bf00425565
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/bf00662431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033394583
    144 https://doi.org/10.1007/bf00662431
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1038/298623a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026714891
    147 https://doi.org/10.1038/298623a0
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1038/303179a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
    150 https://doi.org/10.1038/303179a0
    151 rdf:type schema:CreativeWork
    152 https://doi.org/10.1002/j.1460-2075.1984.tb02032.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183680
    153 rdf:type schema:CreativeWork
    154 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183716
    155 rdf:type schema:CreativeWork
    156 https://doi.org/10.1002/j.1460-2075.1985.tb03996.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1080025490
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1016/0022-2836(80)90095-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024871846
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/0092-8674(80)90178-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022719309
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/0092-8674(81)90176-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009056250
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/0092-8674(81)90343-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036391311
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/0092-8674(82)90181-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043719981
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/0092-8674(82)90464-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036096412
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/0378-1119(81)90143-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026467580
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/0378-1119(84)90122-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000852526
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/s0092-8674(80)80049-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016541918
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1073/pnas.77.12.7347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027142782
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1073/pnas.78.7.4151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046272833
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1073/pnas.79.13.4055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039201422
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1073/pnas.79.20.6322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015877276
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1073/pnas.81.12.3825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010484001
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1073/pnas.81.24.8014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037339771
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1073/pnas.82.10.3320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029081242
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1093/nar/13.2.459 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004658910
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016020981
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1126/science.223.4635.496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062528157
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1126/science.227.4691.1229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062529900
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1126/science.6251546 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062635866
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1126/science.6289436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062636078
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1146/annurev.pp.35.060184.002131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030599579
    207 rdf:type schema:CreativeWork
     




    Preview window. Press ESC to close (or click here)


    ...