Cloning, sequencing and analysis of the yeastS. uvarum ERG10 gene encoding acetoacetyl CoA thiolase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-06

AUTHORS

Sylvie Dequin, Remi Gloeckler, Christopher J. Herberte, Françoise Boutelet

ABSTRACT

The ERG10 gene specific to S. uvarum, a brewing yeast, has been cloned by complementation of an S. cerevisiae erg10 mutant. S. uvarum contains two different ERG10 genes. One of these is similar to the S. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The cloned ERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolated tsm0115 mutants. Northern blot and sequence analysis indicate that the ERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase. More... »

PAGES

471-478

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02427752

DOI

http://dx.doi.org/10.1007/bf02427752

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045146906

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2900076


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58 schema:description The ERG10 gene specific to S. uvarum, a brewing yeast, has been cloned by complementation of an S. cerevisiae erg10 mutant. S. uvarum contains two different ERG10 genes. One of these is similar to the S. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The cloned ERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolated tsm0115 mutants. Northern blot and sequence analysis indicate that the ERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.
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