Synthesis of cytochromec oxidase polypeptides in anEscherichia coli cell-free system directed bySaccharomyces cerevisiae mitochondrial DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

Allan H. Scragg, David Y. Thomas

ABSTRACT

Using purified yeast mitochondrial DNA as a template forE. coli RNA polymerase (holoenzyme) complementary mitochondrial RNA has been synthesized in vitro. This RNA has been used to direct a low backgroundE. coli S-30 protein-synthesizing system. The synthesis of mitochondrial polypeptides has been detected by using antiserum raised against purified cytochromec oxidase holoenzyme and shown to be specific for this antigen. The antiserum-antigen complex was dissociated and subject to SDS-polyacrylamide gel electrophoresis and the presence of 3 polypeptides of 39, 31, and 26×103 daltons molecular weight demonstrated, which correspond to the subunits synthesized by mitochondria in whole cells which are inhibited with cycloheximide. More... »

PAGES

81-86

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02425328

DOI

http://dx.doi.org/10.1007/bf02425328

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022286081

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/189180


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