Amylolytic activity in stored potato tubers. 1. Estimation usingp-nitrophenyloligosaccharides View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09

AUTHORS

M. Patricia Cochrane, Carol M. Duffus, M. J. Allison, G. R. Mackay

ABSTRACT

Methods for the estimation of amylolytic activity are reviewed. A procedure for the routine extraction of amylolytic activity from freeze-dried powder prepared from potato tubers is described. The extraction medium is buffered at pH 7.0 and contains glycerol, dithiothreitol, calcium chloride and the non-ionic detergent, nonidet P-40. α-Amylase activity and exoamylolytic activity were estimated in crude extracts of potato tubers using the substrates, blockedp-nitrophenyl-maltoheptaoside andp-nitrophenyl-maltopentaoside respectively. These substrates are included in kits supplied by Biocon, (UK) Ltd to measure the α- and ß-amylase activity in cereals. The validity of using these kits for the determination of α- and ß-amylase activity in potato tuber tissue is discussed. More... »

PAGES

325-332

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02360506

DOI

http://dx.doi.org/10.1007/bf02360506

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044109145


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