Radioimmunological determination of pancreatic and gut glucagon in plasma View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1971-02

AUTHORS

Lise G. Heding

ABSTRACT

A radioimmunoassay has been developed for glucagon. The antibodies raised in rabbits were sensitive to 0.01 ng of glueagon in a volume of 100 μl. The free and the antibody-bound125I-glucagon were separated by addition of ethanol or by paper chromatography on Whatman 3 MM. Two types of antisera were used: one that did not react, with gut GLI (glucagon-like immunoreactivity), and another one that reacted with both the pancreatic glucagon and the gut GLI, giving identical dilution curves for the two substances. Exogenous pan-creatic glueagon and gut GLI experienced a high degree of degradation during the preparation of plasma unless trasylol was added. A degradation of endogenous pancreatic and gut GLI could also be shown in the absence of trasylol. The damage to125I-glueagon occurring during incubation in plasma was eliminated by using an easy plasma extraction method yielding an approximately 80% recovery of pancreatic glueagon and gut GLI. Normal human fasting plasma extracts contained 0.26 ± 0.024 ng equiv, of pancreatic GLI and 0.38±0.048 ng equiv. of gut GLI (mean of 21 samples ± s.e.m.). During OGTT the pancreatic GLI decreased while the gut GLI increased. The specificity and validity of glueagon determinations are discussed. More... »

PAGES

10-19

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02346248

DOI

http://dx.doi.org/10.1007/bf02346248

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009810465

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5313395


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