A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-04

AUTHORS

J. M. Franco, M. A. Murado, Maria I. G. Siso, J. Miron, Maria P. Gonzalez

ABSTRACT

In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent. More... »

PAGES

328-332

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02321279

DOI

http://dx.doi.org/10.1007/bf02321279

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029912385


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0303", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Macromolecular and Materials Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Franco", 
        "givenName": "J. M.", 
        "id": "sg:person.01276342541.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276342541.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murado", 
        "givenName": "M. A.", 
        "id": "sg:person.01342261126.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342261126.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Siso", 
        "givenName": "Maria I. G.", 
        "id": "sg:person.011245364025.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miron", 
        "givenName": "J.", 
        "id": "sg:person.01226255770.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226255770.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gonzalez", 
        "givenName": "Maria P.", 
        "id": "sg:person.016070570472.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016070570472.33"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00227560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000569211", 
          "https://doi.org/10.1007/bf00227560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00227560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000569211", 
          "https://doi.org/10.1007/bf00227560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9780470122570.ch7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021255042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02324863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021878313", 
          "https://doi.org/10.1007/bf02324863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02324863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021878313", 
          "https://doi.org/10.1007/bf02324863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260230618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036788617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260270308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043195119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01087445", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052442717", 
          "https://doi.org/10.1007/bf01087445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01087445", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052442717", 
          "https://doi.org/10.1007/bf01087445"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1989-04", 
    "datePublishedReg": "1989-04-01", 
    "description": "In the hydrolysis of soluble starch by mixtures of \u03b1-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02321279", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1015080", 
        "issn": [
          "0009-5893", 
          "1612-1112"
        ], 
        "name": "Chromatographia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7-8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "27"
      }
    ], 
    "name": "A HPLC method for specific determination of\u03b1-amylase and glucoamylase in complex enzymatic preparations", 
    "pagination": "328-332", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8e80066916ece044c9fcab262c8f0bde9dc4a53625437e395da32e73b6e16a86"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02321279"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029912385"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02321279", 
      "https://app.dimensions.ai/details/publication/pub.1029912385"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000365_0000000365/records_71673_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02321279"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02321279'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02321279'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02321279'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02321279'


 

This table displays all metadata directly associated to this object as RDF triples.

117 TRIPLES      21 PREDICATES      33 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02321279 schema:about anzsrc-for:03
2 anzsrc-for:0303
3 schema:author N1b228b246e2747d6b8386d922a98e28a
4 schema:citation sg:pub.10.1007/bf00227560
5 sg:pub.10.1007/bf01087445
6 sg:pub.10.1007/bf02324863
7 https://doi.org/10.1002/9780470122570.ch7
8 https://doi.org/10.1002/bit.260230618
9 https://doi.org/10.1002/bit.260270308
10 schema:datePublished 1989-04
11 schema:datePublishedReg 1989-04-01
12 schema:description In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent.
13 schema:genre research_article
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf Nc90c94f04c5a4d358b86018ee8a948bd
17 Ncbb2009a48a845fa9dca97398d44aae0
18 sg:journal.1015080
19 schema:name A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations
20 schema:pagination 328-332
21 schema:productId N01be28597d324d32b2d5c3442a76b656
22 N06aea2dd060a485da67bffa73c9039cf
23 N9f83cfef73dc4621b4e519ad3503dc29
24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029912385
25 https://doi.org/10.1007/bf02321279
26 schema:sdDatePublished 2019-04-11T12:56
27 schema:sdLicense https://scigraph.springernature.com/explorer/license/
28 schema:sdPublisher N53d5321d63f4432fb6a4b3c2698a947a
29 schema:url http://link.springer.com/10.1007/BF02321279
30 sgo:license sg:explorer/license/
31 sgo:sdDataset articles
32 rdf:type schema:ScholarlyArticle
33 N00b5f12a6f42427a85715fd13b806cb6 schema:name Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain
34 rdf:type schema:Organization
35 N01be28597d324d32b2d5c3442a76b656 schema:name dimensions_id
36 schema:value pub.1029912385
37 rdf:type schema:PropertyValue
38 N06aea2dd060a485da67bffa73c9039cf schema:name readcube_id
39 schema:value 8e80066916ece044c9fcab262c8f0bde9dc4a53625437e395da32e73b6e16a86
40 rdf:type schema:PropertyValue
41 N0f3aaff7c83d48f29fa2ec40bee3356e schema:name Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain
42 rdf:type schema:Organization
43 N14ddf554b1a346b4b530a07b04036c30 rdf:first sg:person.01226255770.19
44 rdf:rest Nbc196acde78b4efbaf51c8d3551d657b
45 N1b228b246e2747d6b8386d922a98e28a rdf:first sg:person.01276342541.90
46 rdf:rest N4b325625d12f4b4689da381aafc2447d
47 N1ed269369a6a4f43879930d81da058b2 rdf:first sg:person.011245364025.32
48 rdf:rest N14ddf554b1a346b4b530a07b04036c30
49 N37c22ad6d8ae4b799054e43bd19ddd7d schema:name Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain
50 rdf:type schema:Organization
51 N4b325625d12f4b4689da381aafc2447d rdf:first sg:person.01342261126.57
52 rdf:rest N1ed269369a6a4f43879930d81da058b2
53 N53d5321d63f4432fb6a4b3c2698a947a schema:name Springer Nature - SN SciGraph project
54 rdf:type schema:Organization
55 N7b6c882b01104a39ad6af7087550423a schema:name Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain
56 rdf:type schema:Organization
57 N9f83cfef73dc4621b4e519ad3503dc29 schema:name doi
58 schema:value 10.1007/bf02321279
59 rdf:type schema:PropertyValue
60 Nbc196acde78b4efbaf51c8d3551d657b rdf:first sg:person.016070570472.33
61 rdf:rest rdf:nil
62 Nc41005f557fa4df394d0e938fe7257b7 schema:name Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208, Vigo, Spain
63 rdf:type schema:Organization
64 Nc90c94f04c5a4d358b86018ee8a948bd schema:volumeNumber 27
65 rdf:type schema:PublicationVolume
66 Ncbb2009a48a845fa9dca97398d44aae0 schema:issueNumber 7-8
67 rdf:type schema:PublicationIssue
68 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
69 schema:name Chemical Sciences
70 rdf:type schema:DefinedTerm
71 anzsrc-for:0303 schema:inDefinedTermSet anzsrc-for:
72 schema:name Macromolecular and Materials Chemistry
73 rdf:type schema:DefinedTerm
74 sg:journal.1015080 schema:issn 0009-5893
75 1612-1112
76 schema:name Chromatographia
77 rdf:type schema:Periodical
78 sg:person.011245364025.32 schema:affiliation Nc41005f557fa4df394d0e938fe7257b7
79 schema:familyName Siso
80 schema:givenName Maria I. G.
81 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32
82 rdf:type schema:Person
83 sg:person.01226255770.19 schema:affiliation N0f3aaff7c83d48f29fa2ec40bee3356e
84 schema:familyName Miron
85 schema:givenName J.
86 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226255770.19
87 rdf:type schema:Person
88 sg:person.01276342541.90 schema:affiliation N00b5f12a6f42427a85715fd13b806cb6
89 schema:familyName Franco
90 schema:givenName J. M.
91 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276342541.90
92 rdf:type schema:Person
93 sg:person.01342261126.57 schema:affiliation N37c22ad6d8ae4b799054e43bd19ddd7d
94 schema:familyName Murado
95 schema:givenName M. A.
96 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342261126.57
97 rdf:type schema:Person
98 sg:person.016070570472.33 schema:affiliation N7b6c882b01104a39ad6af7087550423a
99 schema:familyName Gonzalez
100 schema:givenName Maria P.
101 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016070570472.33
102 rdf:type schema:Person
103 sg:pub.10.1007/bf00227560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000569211
104 https://doi.org/10.1007/bf00227560
105 rdf:type schema:CreativeWork
106 sg:pub.10.1007/bf01087445 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052442717
107 https://doi.org/10.1007/bf01087445
108 rdf:type schema:CreativeWork
109 sg:pub.10.1007/bf02324863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021878313
110 https://doi.org/10.1007/bf02324863
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1002/9780470122570.ch7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021255042
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1002/bit.260230618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036788617
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1002/bit.260270308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043195119
117 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...