A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations View Full Text


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Article Info

DATE

1989-04

AUTHORS

J. M. Franco, M. A. Murado, Maria I. G. Siso, J. Miron, Maria P. Gonzalez

ABSTRACT

In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent. More... »

PAGES

328-332

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02321279

DOI

http://dx.doi.org/10.1007/bf02321279

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029912385


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