Somatic embryo cycling: Evaluation of a novel transformation and assay system for seed-specific gene expression in soybean View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-02

AUTHORS

Wennuan Liu, Rebecca S. Torisky, Kay P McAllister, Sergei Avdiushko, David Hildebrand, Glenn B. Collins

ABSTRACT

Somatic embryo cycling, a modification of soybean somatic embryogenic suspension culture, was developed as an efficient and rapid method of producing tissue suitable for stable transformation of soybean germplasm by biolistic particle bombardment. Instead of using immature seed explants, cotyledon-staged somatic embryo hypocotyls were placed on auxin-containing medium, where they initiated new somatic embryos primarily from single epidermal cells. By bombarding hypocotyls prior to initiation of subsequent embryo formation, we have effectively transformed soybean somatic embryos with the reporter genes neomycin phosphotransferase,gb-glucuronidase, and a mammalian stearyl CoA delta-9 desaturase, controlled by a seed-specific promoter. These embryos contain significantly reduced levels of saturated palmitic and stearic fatty acids, and significant amounts of monounsaturated palmitoleic acid, which is not normally abundant in soybean seeds. This study demonstrates the effectiveness of somatic embryo cycling for soybean transformation, and for testing expression of genes for seed-specific proteins. Abnormal flower development in recovered plants is a limitation for application of the technique to produce transgenic seed at present. More... »

PAGES

33-42

References to SciGraph publications

  • 1989-04. A rapid screening technique for determining the lipid composition of soybean seeds in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988-01. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1988-08. Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1988-08. Stable Transformation of Soybean (Glycine Max) by Particle Acceleration in NATURE BIOTECHNOLOGY
  • 1988-08. Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer in NATURE BIOTECHNOLOGY
  • 1989-03. Recovery of primary transformants of soybean in PLANT CELL REPORTS
  • 1989-02. Effect of genotype on somatic embryogenesis from immature cotyledons of soybean in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-10. Transformation of soybean via particle bombardment of embryogenic suspension culture tissue in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1995. Reducing Polyunsaturation in Oils of Transgenic Canola and Soybean in PLANT LIPID METABOLISM
  • 1992-07. Somatic embryogenesis in soybean via somatic embryo cycling in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1992-06. Fatty Acid Alteration by a Δ9 Desaturase in Transgenic Tobacco Tissue in BIO/TECHNOLOGY
  • 1987-05. High-velocity microprojectiles for delivering nucleic acids into living cells in NATURE
  • 1995-09. Cassettes for seed-specific expression tested in transformed embryogenic cultures of soybean in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02318963

    DOI

    http://dx.doi.org/10.1007/bf02318963

    DIMENSIONS

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