Detection by DGGE of a new polymorphism closely linked to the adenomatous polyposis coli region View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-03

AUTHORS

Sylviane Olschwang, Richard Fabre, Pierre Laurent-Puig, Anne Vassal, Bernard Hamelin, Yusuke Nakamura, Gilles Thomas

ABSTRACT

The EF5.44 locus is in close proximity to the chromosome 5 region to which the genetic defect responsible for familial adenomatous polyposis has been mapped. We have devised two oligonucleotides that promote the specific polymerase chain reaction (PCR) amplification of a 365-bp sequence in this region. Analysis by denaturing gradient gel electrophoresis of the resulting fragment has unravelled individual differences that could be identified as a single base pair change in a MnlI restriction site. This PCR assayable polymorphism increases the informativeness at this locus, and should be useful in the presymptomatic diagnosis of familial adenomatous polyposis. More... »

PAGES

658-660

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02265293

DOI

http://dx.doi.org/10.1007/bf02265293

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034698539

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1312993


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