Characterization of DNA sequences constituting the terminal heterochromatin of the chicken Z chromosome View Full Text


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Article Info

DATE

1996-09

AUTHORS

Tetsuya Hori, Yukiko Suzuki, Irina Solovei, Yasushi Saitoh, Nancy Hutchison, Joh-E Ikeda, Herbert Macgregor, Shigeki Mizuno

ABSTRACT

Two clones, pCZTH5-8 and pCZTH12-8, were isolated from a female chicken genomic library by screening with sequences obtained from genomic libraries which had been constructed from a terminal region of a single Z chromosome of chicken utilizing laser microbeam irradiation and PCR amplification. Fluorescence in situ hybridization to the mitotic Z chromosome and the lampbrush ZW bivalent of chicken demonstrated that both the cloned sequences are located in the heterochromatic region of the Z chromosome at the end opposite to the pairing region with the W chromosome. The sequences pCZTH-8 and pCZTH12-8 are distributed widely on both the telomeric bow-like loops (TBL) and the region I (short loops region) of the Z lampbrush chromosome. These clones, pCZTH12-8 particularly notably, hybridized also to the TBLs of lampbrush bivalents 1-4 of chicken. Both sequences are transcribed in the lampbrush stage oocytes on the Z chromosome and on other macrobivalents. The subfragment of pCZTH5-8 which hybridizes to the TBLs and the insert of pCZTH12-8 contain regions that are closely similar in sequence. The pCZTH-8 sequence has no internal repeats and may be part of the 24-kb macrosatellite repeating unit that is evident after Nhel digestion of the genomic DNA. A cloned 24-kb unit, pFN-1, does not show significant DNA curvature, but cytosines of its CpG dinucleotides may be highly methylated in vivo. This contrasts with the repeat sequences of the W heterochromatin which not only have highly methylated CpG but are also strongly curved. The 24-kb unit is repeated about 830 times in the diploid genome of a female chicken, suggesting that nearly the entire terminal heterochromatin on the Z chromosome consists of this macrosatellite family. Sequences of the greater part of the pCZTH-8 are restricted to the genus Gallus but the sequence of one subregion which hybridizes to TBLs is present in the genomes of the order Galliformes. More... »

PAGES

411-426

References to SciGraph publications

  • 1977-06. Ultrastructure of the synaptic autosomes and the ZW bivalent in chicken oocytes in CHROMOSOMA
  • 1992-06. Distribution of XhoI and EcoRI family repetitive DNA sequences into separate domains in the chicken W chromosome in CHROMOSOMA
  • 1993-09. Avian sex chromosomes in the lampbrush form: the ZW lampbrush bivalents from six species of bird in CHROMOSOME RESEARCH
  • 1979-01. Chromosome banding in amphibia in CHROMOSOMA
  • 1985-08. Giant readthrough transcription units at the histone loci on lampbrush chromosomes of the newt Notophthalmus in CHROMOSOMA
  • 1984-03. Genus specificity and extensive methylation of the W chromosome-specific repetitive DNA sequences from the domestic fowl, Gallus gallus domesticus in CHROMOSOMA
  • 1989-10. Presence of female-specific bent-repetitive DNA sequences in the genomes of turkey and pheasant and their interactions with W-protein of chicken in CHROMOSOMA
  • 1980-02. Satellite DNA is transcribed on lampbrush chromosomes in NATURE
  • 1993-11. Identification and localization of two genes on the chicken Z chromosome: implication of evolutionary conservation of the Z chromosome among avian species in CHROMOSOME RESEARCH
  • 1984-03. Chromosome 1 in crested and marbled newts (Triturus) in CHROMOSOMA
  • 1995-03. High-resolution cytological localization of theXhol andEcoRI repeat sequences in the pachytene ZW bivalent of the chicken in CHROMOSOME RESEARCH
  • 1987-12. Nucleotide sequences and unusual electrophoretic behavior of the W chromosome-specific repeating DNA units of the domestic fowl, Gallus gallus domesticus in CHROMOSOMA
  • 1993-06. W-heterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure in GENETICA
  • 1991-10. Occupancy of the majority of DNA in the chicken W chromosome by bent-repetitive sequences in CHROMOSOMA
  • 1994-11. The arrangement and transcription of telomere DNA sequences at the ends of lampbrush chromosomes of birds in CHROMOSOME RESEARCH
  • 1986. Lampbrush Chromosomes in NONE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02265048

    DOI

    http://dx.doi.org/10.1007/bf02265048

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8889240


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