Indirect immunofluorescence and immunodiffusion tests in the detection of antibodies to foot-and-mouth disease virus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-12

AUTHORS

A. A. Schudel, G. Zuloaga, F. M. Fernandez, N. Fondevila, M. V. Borca

ABSTRACT

The antibody response detected by indirect immunofluorescence (IIF) as well as that directed against 140 S and virus infection associated antigen (VIA), as detected by agar immunodiffusion, was studied in three mammal species susceptible to Foot and Mouth Disease Virus, after challenge with living virus, immunization and hyperimmunization with inactivated virus, and immunization followed by challenge. By spot indirect immunofluorescence, antibodies were detected only in animals undergoing an active infection, and were not detected in immunized or hyperimmunized animals. This behaviour was similar to that of the anti-VIA antibodies in the same groups of animals and differed from that of anti-140 S antibodies. It appeared that spot indirect immunofluorescence for the detection of VIA antigen is comparable to the immunodiffusion test, but the speed of IIF and the possibility of handling many samples make it more practical. More... »

PAGES

15-23

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02215124

DOI

http://dx.doi.org/10.1007/bf02215124

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010973140

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2983488


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