Two T-DNA's co-transformed intoBrassica napus by a doubleAgrobacterium tumefaciens infection are mainly integrated at the same locus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09

AUTHORS

Marc De Block, Dirk Debrouwer

ABSTRACT

Hypocotyl explants of threeBrassica napus varieties were infected with two nopaline typeAgrobacterium strains each carrying a distinct disarmed T-DNA containing different selectable markers. Selection was done for only one of the markers, after which the regenerated plants were screened for the presence of the second marker. High co-transformation frequencies of both T-DNA's were obtained (39%-85% of the transformants). Where the two T-DNA's were integrated linked, they were usually present in an inverted orientation relative to each other; in all of the cases observed the two right borders were adjacent. Tandem orientations occurred less frequently. The T-DNA's were mainly integrated as intact copies and deletions did not often occur. The co-transformation system described favors a genetically linked integration of the two T-DNA's (78%), although in a single transformed plant both linked and unlinked copies of both T-DNA's may be present. More... »

PAGES

257-263

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02190610

DOI

http://dx.doi.org/10.1007/bf02190610

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045259980

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24213168


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