Site-selected insertional mutagenesis of tomato with maizeAc andDs elements View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-08

AUTHORS

M. B. Cooley, J. I. Yoder, A. P. Goldsbrough, D. W. Still

ABSTRACT

Site-selected insertion (SSI) is a PCR-based technique which uses primers located within the transposon and a target gene for detection of transposon insertions into cloned genes. We screened tomato plants bearing single or multiple copies of maize Ac or Ds transposable elements for somatic insertions at one close-range target and two long-range targets. Eight close-range Ds insertions near the right border of the T-DNA were recovered. Sequence analysis showed a precise junction between the transposon and the target for all insertions. Two insertions in separate plants occurred at the same site, but others appeared dispersed in the region of the right T-DNA border with no target specificity. However, insertions showed a preference for one orientation of the transposon. Use of plants with multiple Ac (HiAc) or Ds (HiDs) elements allowed detection of somatic insertions at two single-copy genes, PG (polygalacturonase) and DFR (dihydroflavonol 4-reductase). Certain HiDs plants showed much higher rates of insertion into PG than others. Insertions in PG and DFR were found throughout the gene regions monitored and, with the exception of one insertion in PG, the junctions between transposon and target were exact. SSI analysis of progeny from the HiDs parents revealed that in some cases the tendency to incur high levels of somatic insertions in PG was inherited. Inheritance of this character is an indication that SSI could be used to direct a search for germinal PG insertions in tomato. More... »

PAGES

184-194

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02173219

DOI

http://dx.doi.org/10.1007/bf02173219

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052981468

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8804392


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alcohol Oxidoreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Transposable Elements", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lycopersicon esculentum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Insertional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polygalacturonase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zea mays", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Department of Vegetable Crops, University of California, 95616, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cooley", 
        "givenName": "M. B.", 
        "id": "sg:person.01105671710.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105671710.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Department of Vegetable Crops, University of California, 95616, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoder", 
        "givenName": "J. I.", 
        "id": "sg:person.0702635166.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702635166.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "PBI Cambridge Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goldsbrough", 
        "givenName": "A. P.", 
        "id": "sg:person.01271340010.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271340010.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Arizona", 
          "id": "https://www.grid.ac/institutes/grid.134563.6", 
          "name": [
            "Yuma Agricultural Center, University of Arizona, 85364, Yuma, AZ, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Still", 
        "givenName": "D. W.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.92.18.8149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000166152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.13.2.902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005857118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.23.9402", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006059087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/g95-033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012284654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.7.1.75", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013200978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90116-t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013914002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(94)04051-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015284318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4899-1265-7_13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015342745", 
          "https://doi.org/10.1007/978-1-4899-1265-7_13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.7.3.287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017945861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00402933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018811523", 
          "https://doi.org/10.1007/bf00402933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00402933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018811523", 
          "https://doi.org/10.1007/bf00402933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.2.8.709", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019481518"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.pp.43.060192.000405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026962313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(94)90799-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031668612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(94)90799-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031668612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.2.8.723", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032854823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.81.7.1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034589758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.25.120191.001133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034743521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1193-1286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035170318", 
          "https://doi.org/10.1038/nbt1193-1286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00339594", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035710820", 
          "https://doi.org/10.1007/bf00339594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00023968", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037264774", 
          "https://doi.org/10.1007/bf00023968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00023968", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037264774", 
          "https://doi.org/10.1007/bf00023968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1992.t01-40-00999.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040858211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.85.23.8805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041146261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00226158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042225402", 
          "https://doi.org/10.1007/bf00226158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00330561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043476284", 
          "https://doi.org/10.1007/bf00330561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2296(08)60194-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049079281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1365-3040.ep11602134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049589405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/biomet/52.3-4.591", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059417484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.105.2.491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060840363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.268.5211.686", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062550110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.173.8.2608-2616.1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062720349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467815"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075516986", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075583615", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077269580", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077356267", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079411049", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082849195", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-08", 
    "datePublishedReg": "1996-08-01", 
    "description": "Site-selected insertion (SSI) is a PCR-based technique which uses primers located within the transposon and a target gene for detection of transposon insertions into cloned genes. We screened tomato plants bearing single or multiple copies of maize Ac or Ds transposable elements for somatic insertions at one close-range target and two long-range targets. Eight close-range Ds insertions near the right border of the T-DNA were recovered. Sequence analysis showed a precise junction between the transposon and the target for all insertions. Two insertions in separate plants occurred at the same site, but others appeared dispersed in the region of the right T-DNA border with no target specificity. However, insertions showed a preference for one orientation of the transposon. Use of plants with multiple Ac (HiAc) or Ds (HiDs) elements allowed detection of somatic insertions at two single-copy genes, PG (polygalacturonase) and DFR (dihydroflavonol 4-reductase). Certain HiDs plants showed much higher rates of insertion into PG than others. Insertions in PG and DFR were found throughout the gene regions monitored and, with the exception of one insertion in PG, the junctions between transposon and target were exact. SSI analysis of progeny from the HiDs parents revealed that in some cases the tendency to incur high levels of somatic insertions in PG was inherited. Inheritance of this character is an indication that SSI could be used to direct a search for germinal PG insertions in tomato.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02173219", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "252"
      }
    ], 
    "name": "Site-selected insertional mutagenesis of tomato with maizeAc andDs elements", 
    "pagination": "184-194", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fc5da22641248e866b2fd844888287dbcdd6af8fb7a9c5d4693bfa7308510599"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8804392"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0125036"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02173219"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1052981468"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02173219", 
      "https://app.dimensions.ai/details/publication/pub.1052981468"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54015_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02173219"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02173219'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02173219'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02173219'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02173219'


 

This table displays all metadata directly associated to this object as RDF triples.

269 TRIPLES      21 PREDICATES      82 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02173219 schema:about N1180df774e77428eb9e9c8fb81f8de30
2 N203469212845466caf14aca72d56dbe4
3 N4bcf42df8f714a32aeb017296771b586
4 N524cf186d8d34ef3a4535a8456aa5875
5 N5e1b36c1f3f846c785a9cc9206f406ab
6 N7b101b50dcfb468083ffd077328955ce
7 N8f3e7538a4374b78bc0e6bbd6b9e6afe
8 N9bd5847d53f644b79131b4d467f08e27
9 Na61c79f0f0bc40b2bf64e89d6a15714f
10 Naa57f4f2b55e4f03aaef3f50a1643da8
11 Nb47f863379a34ba28f3fb9c327d590ff
12 Nb545e10bf8004323bc671892905e0ac5
13 Nc3924aba3ce24ba0b7e45367e42afe22
14 Nd6e8bc087e384e96b05321f91e2f26db
15 Ndfe56d5394b04447978ef43483d55663
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author Na11290c3f3a645e982e7fe1681d3c035
19 schema:citation sg:pub.10.1007/978-1-4899-1265-7_13
20 sg:pub.10.1007/bf00023968
21 sg:pub.10.1007/bf00226158
22 sg:pub.10.1007/bf00330561
23 sg:pub.10.1007/bf00339594
24 sg:pub.10.1007/bf00402933
25 sg:pub.10.1038/nbt1193-1286
26 https://app.dimensions.ai/details/publication/pub.1075516986
27 https://app.dimensions.ai/details/publication/pub.1075583615
28 https://app.dimensions.ai/details/publication/pub.1077269580
29 https://app.dimensions.ai/details/publication/pub.1077356267
30 https://app.dimensions.ai/details/publication/pub.1079411049
31 https://app.dimensions.ai/details/publication/pub.1082849195
32 https://doi.org/10.1016/0092-8674(92)90116-t
33 https://doi.org/10.1016/0168-9452(94)04051-6
34 https://doi.org/10.1016/0378-1119(94)90799-4
35 https://doi.org/10.1016/s0065-2296(08)60194-9
36 https://doi.org/10.1046/j.1365-313x.1992.t01-40-00999.x
37 https://doi.org/10.1073/pnas.81.7.1991
38 https://doi.org/10.1073/pnas.85.23.8805
39 https://doi.org/10.1073/pnas.86.23.9402
40 https://doi.org/10.1073/pnas.92.18.8149
41 https://doi.org/10.1093/biomet/52.3-4.591
42 https://doi.org/10.1104/pp.105.2.491
43 https://doi.org/10.1105/tpc.2.8.709
44 https://doi.org/10.1105/tpc.2.8.723
45 https://doi.org/10.1105/tpc.7.1.75
46 https://doi.org/10.1105/tpc.7.3.287
47 https://doi.org/10.1111/1365-3040.ep11602134
48 https://doi.org/10.1126/science.268.5211.686
49 https://doi.org/10.1128/jb.173.8.2608-2616.1991
50 https://doi.org/10.1128/mcb.13.2.902
51 https://doi.org/10.1139/g95-033
52 https://doi.org/10.1146/annurev.ge.25.120191.001133
53 https://doi.org/10.1146/annurev.pp.43.060192.000405
54 https://doi.org/10.2307/3869170
55 https://doi.org/10.2307/3869839
56 https://doi.org/10.2307/3869851
57 schema:datePublished 1996-08
58 schema:datePublishedReg 1996-08-01
59 schema:description Site-selected insertion (SSI) is a PCR-based technique which uses primers located within the transposon and a target gene for detection of transposon insertions into cloned genes. We screened tomato plants bearing single or multiple copies of maize Ac or Ds transposable elements for somatic insertions at one close-range target and two long-range targets. Eight close-range Ds insertions near the right border of the T-DNA were recovered. Sequence analysis showed a precise junction between the transposon and the target for all insertions. Two insertions in separate plants occurred at the same site, but others appeared dispersed in the region of the right T-DNA border with no target specificity. However, insertions showed a preference for one orientation of the transposon. Use of plants with multiple Ac (HiAc) or Ds (HiDs) elements allowed detection of somatic insertions at two single-copy genes, PG (polygalacturonase) and DFR (dihydroflavonol 4-reductase). Certain HiDs plants showed much higher rates of insertion into PG than others. Insertions in PG and DFR were found throughout the gene regions monitored and, with the exception of one insertion in PG, the junctions between transposon and target were exact. SSI analysis of progeny from the HiDs parents revealed that in some cases the tendency to incur high levels of somatic insertions in PG was inherited. Inheritance of this character is an indication that SSI could be used to direct a search for germinal PG insertions in tomato.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree false
63 schema:isPartOf N778ffb3de87a4e7faeaeb0d2eb033874
64 Ne444082b00b3493fb79d1f8f63b242f2
65 sg:journal.1297380
66 schema:name Site-selected insertional mutagenesis of tomato with maizeAc andDs elements
67 schema:pagination 184-194
68 schema:productId N275f91fb2c07464ba684b8e8b73be5ca
69 N28563e64aa1d4fab93d6e6f2d711134c
70 N32147a5bc783459aa363f2aede9077fb
71 Nbbd35c55eeb54b83a1c7c36a085d6ce3
72 Nc86f87e301344d0ba2d78471941b3dff
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052981468
74 https://doi.org/10.1007/bf02173219
75 schema:sdDatePublished 2019-04-11T12:15
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher N4ed31abee4e64d92baa8bc3519570bbe
78 schema:url http://link.springer.com/10.1007/BF02173219
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N06010493cf6c4387acb4192dbf4c1321 rdf:first sg:person.0702635166.94
83 rdf:rest N57241e0c8f3e447d8dbc3a075aa085a4
84 N1180df774e77428eb9e9c8fb81f8de30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name DNA Transposable Elements
86 rdf:type schema:DefinedTerm
87 N1953382bb89e49f388127700cdef7861 schema:affiliation https://www.grid.ac/institutes/grid.134563.6
88 schema:familyName Still
89 schema:givenName D. W.
90 rdf:type schema:Person
91 N203469212845466caf14aca72d56dbe4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Genes, Plant
93 rdf:type schema:DefinedTerm
94 N275f91fb2c07464ba684b8e8b73be5ca schema:name dimensions_id
95 schema:value pub.1052981468
96 rdf:type schema:PropertyValue
97 N28563e64aa1d4fab93d6e6f2d711134c schema:name readcube_id
98 schema:value fc5da22641248e866b2fd844888287dbcdd6af8fb7a9c5d4693bfa7308510599
99 rdf:type schema:PropertyValue
100 N2ef1c51d96b44f9382a309e4a4b58a3d schema:name PBI Cambridge Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK
101 rdf:type schema:Organization
102 N32147a5bc783459aa363f2aede9077fb schema:name doi
103 schema:value 10.1007/bf02173219
104 rdf:type schema:PropertyValue
105 N4bcf42df8f714a32aeb017296771b586 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Zea mays
107 rdf:type schema:DefinedTerm
108 N4ed31abee4e64d92baa8bc3519570bbe schema:name Springer Nature - SN SciGraph project
109 rdf:type schema:Organization
110 N524cf186d8d34ef3a4535a8456aa5875 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Mutagenesis, Insertional
112 rdf:type schema:DefinedTerm
113 N57241e0c8f3e447d8dbc3a075aa085a4 rdf:first sg:person.01271340010.30
114 rdf:rest Nd2426c2b1e724578b787c3651c94c1c8
115 N5e1b36c1f3f846c785a9cc9206f406ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name DNA Primers
117 rdf:type schema:DefinedTerm
118 N778ffb3de87a4e7faeaeb0d2eb033874 schema:volumeNumber 252
119 rdf:type schema:PublicationVolume
120 N7b101b50dcfb468083ffd077328955ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Cloning, Molecular
122 rdf:type schema:DefinedTerm
123 N8f3e7538a4374b78bc0e6bbd6b9e6afe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Base Sequence
125 rdf:type schema:DefinedTerm
126 N9bd5847d53f644b79131b4d467f08e27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Alcohol Oxidoreductases
128 rdf:type schema:DefinedTerm
129 Na11290c3f3a645e982e7fe1681d3c035 rdf:first sg:person.01105671710.14
130 rdf:rest N06010493cf6c4387acb4192dbf4c1321
131 Na61c79f0f0bc40b2bf64e89d6a15714f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Blotting, Southern
133 rdf:type schema:DefinedTerm
134 Naa57f4f2b55e4f03aaef3f50a1643da8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Chromosome Mapping
136 rdf:type schema:DefinedTerm
137 Nb47f863379a34ba28f3fb9c327d590ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name DNA, Bacterial
139 rdf:type schema:DefinedTerm
140 Nb545e10bf8004323bc671892905e0ac5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Polygalacturonase
142 rdf:type schema:DefinedTerm
143 Nbbd35c55eeb54b83a1c7c36a085d6ce3 schema:name pubmed_id
144 schema:value 8804392
145 rdf:type schema:PropertyValue
146 Nc3924aba3ce24ba0b7e45367e42afe22 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Plant Proteins
148 rdf:type schema:DefinedTerm
149 Nc86f87e301344d0ba2d78471941b3dff schema:name nlm_unique_id
150 schema:value 0125036
151 rdf:type schema:PropertyValue
152 Nd2426c2b1e724578b787c3651c94c1c8 rdf:first N1953382bb89e49f388127700cdef7861
153 rdf:rest rdf:nil
154 Nd6e8bc087e384e96b05321f91e2f26db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Lycopersicon esculentum
156 rdf:type schema:DefinedTerm
157 Ndfe56d5394b04447978ef43483d55663 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Molecular Sequence Data
159 rdf:type schema:DefinedTerm
160 Ne444082b00b3493fb79d1f8f63b242f2 schema:issueNumber 1-2
161 rdf:type schema:PublicationIssue
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
166 schema:name Genetics
167 rdf:type schema:DefinedTerm
168 sg:journal.1297380 schema:issn 1432-1874
169 1617-4615
170 schema:name Molecular Genetics and Genomics
171 rdf:type schema:Periodical
172 sg:person.01105671710.14 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
173 schema:familyName Cooley
174 schema:givenName M. B.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105671710.14
176 rdf:type schema:Person
177 sg:person.01271340010.30 schema:affiliation N2ef1c51d96b44f9382a309e4a4b58a3d
178 schema:familyName Goldsbrough
179 schema:givenName A. P.
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271340010.30
181 rdf:type schema:Person
182 sg:person.0702635166.94 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
183 schema:familyName Yoder
184 schema:givenName J. I.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702635166.94
186 rdf:type schema:Person
187 sg:pub.10.1007/978-1-4899-1265-7_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015342745
188 https://doi.org/10.1007/978-1-4899-1265-7_13
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf00023968 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037264774
191 https://doi.org/10.1007/bf00023968
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/bf00226158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042225402
194 https://doi.org/10.1007/bf00226158
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/bf00330561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043476284
197 https://doi.org/10.1007/bf00330561
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/bf00339594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035710820
200 https://doi.org/10.1007/bf00339594
201 rdf:type schema:CreativeWork
202 sg:pub.10.1007/bf00402933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018811523
203 https://doi.org/10.1007/bf00402933
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nbt1193-1286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035170318
206 https://doi.org/10.1038/nbt1193-1286
207 rdf:type schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1075516986 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1075583615 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1077269580 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1077356267 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1079411049 schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1082849195 schema:CreativeWork
214 https://doi.org/10.1016/0092-8674(92)90116-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1013914002
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0168-9452(94)04051-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015284318
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0378-1119(94)90799-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031668612
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/s0065-2296(08)60194-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049079281
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1046/j.1365-313x.1992.t01-40-00999.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040858211
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1073/pnas.81.7.1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034589758
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1073/pnas.85.23.8805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041146261
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.86.23.9402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006059087
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.92.18.8149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000166152
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1093/biomet/52.3-4.591 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059417484
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1104/pp.105.2.491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060840363
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1105/tpc.2.8.709 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019481518
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1105/tpc.2.8.723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032854823
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1105/tpc.7.1.75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013200978
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1105/tpc.7.3.287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017945861
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1111/1365-3040.ep11602134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049589405
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1126/science.268.5211.686 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062550110
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1128/jb.173.8.2608-2616.1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062720349
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1128/mcb.13.2.902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005857118
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1139/g95-033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012284654
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1146/annurev.ge.25.120191.001133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034743521
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1146/annurev.pp.43.060192.000405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026962313
257 rdf:type schema:CreativeWork
258 https://doi.org/10.2307/3869170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467233
259 rdf:type schema:CreativeWork
260 https://doi.org/10.2307/3869839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467805
261 rdf:type schema:CreativeWork
262 https://doi.org/10.2307/3869851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467815
263 rdf:type schema:CreativeWork
264 https://www.grid.ac/institutes/grid.134563.6 schema:alternateName University of Arizona
265 schema:name Yuma Agricultural Center, University of Arizona, 85364, Yuma, AZ, USA
266 rdf:type schema:Organization
267 https://www.grid.ac/institutes/grid.27860.3b schema:alternateName University of California, Davis
268 schema:name Department of Vegetable Crops, University of California, 95616, Davis, CA, USA
269 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...