Metabolic studies on vaccinia-virus-infected oligodendrocytes in brain cell cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-09

AUTHORS

C. F. Beranek, N. Herschkowitz

ABSTRACT

Twelve-day-old cultures of dissociated newborn mouse brain were infected with neurotropic vaccinia virus strain WR. Using the indirect immuno-fluorescence staining technique the total destruction of galactocerebroside (GL) or myelin basic protein (MBP)-positive oligodendrocytes could be detected after 72 h of infection. The activity of the oligodendrocyte-specific enzymes, cerebroside sulfotransferase (CST) and 2'3'-cyclic nucleotide 3'-phosphohydrolase (CNP), was 27% and 17% respectively of the activity in noninfected controls. This reduction was not a result of viral-induced inhibition of host protein synthesis. In cultures treated with puromycin GC- and MBP- positive oligodendrocytes were detectable at a time at which no CST or CNP activity could detected. More... »

PAGES

95-103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02124823

DOI

http://dx.doi.org/10.1007/bf02124823

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001974846

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6208465


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