Bone growth in organ culture: Effects of phosphate and other nutrients on bone and cartilage View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-12

AUTHORS

Patricia J. Bingham, Lawrence G. Raisz

ABSTRACT

A method for studying the growth of fetal rat long bones in a chemically defined medium in organ culture is described. Cartilage ends and bone shafts were analyzed separately for growth and mineralization by measuring the collagen, calcium, and phosphate content, dry weight, and incorporation of labeled proline into hydroxyproline. Growth and mineralization of the bone shaft were slower in a chemically defined medium thanin vivo. Growth could be enhanced by supplementation of the medium with non-essential amino acids, albumin or serum. Cartilage ends showed a greater increase in weight and collagen content than the shafts, and medium supplements had less effect on their growth. Bone shaft growth and mineralization were enhanced by increasing medium phosphate concentration over a range of 1.5 to 4.5 mM whether or not the medium was supplemented with serum or albumin. At a low medium calcium concentration (0.5 mM) bone shaft growth and mineralization were impaired. At a low magnesium concentration (0.5 mM) mineralization was enhanced, but growth was impaired. More... »

PAGES

31-48

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02060281

DOI

http://dx.doi.org/10.1007/bf02060281

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018072573

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4820236


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