Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-06

AUTHORS

C. Arteaga de Murphy, L. Meléndez-Alafort, O. Martínez-Rivero, E. Gómez, G. Ferro-Flores

ABSTRACT

A new technetium labeling method for immunoglobulin reduced with tris(2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobulina IgG source was assayed for purity and optimum reagent's concentration and incubation times were determined. It was purified by column chromatography and labelled with Sn2+ reduced technetium in the presence of MDP. The kit is easy to prepare, labeling efficiency is >(97±1.9) % and stable for 6 hours. The immunoreactivity of the99mTc-IgG was verified by electrophoresis and Western blot tests. The IgG retained its structure after both the reducing and labeling processes and it was the only labeled species. More... »

PAGES

41-45

References to SciGraph publications

  • 1969-02. Reduction of biological substances by water-soluble phosphines: Gamma-globulin (IgG) in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 1995-03. Radiolabeling with technetium-99m to study high-capacity and low-capacity biochemical systems in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02035345

    DOI

    http://dx.doi.org/10.1007/bf02035345

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007274642


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Analytical Chemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n", 
              "id": "https://www.grid.ac/institutes/grid.416850.e", 
              "name": [
                "Radiofarmacia, Departamento de Medicina Nuclear, Instituto Nacional de Nutrici\u00f3n Salvador Zubir\u00e1n, INNSZ, M\u00e9xico, D. F., Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arteaga de Murphy", 
            "givenName": "C.", 
            "id": "sg:person.01275074523.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275074523.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n", 
              "id": "https://www.grid.ac/institutes/grid.416850.e", 
              "name": [
                "Radiofarmacia, Departamento de Medicina Nuclear, Instituto Nacional de Nutrici\u00f3n Salvador Zubir\u00e1n, INNSZ, M\u00e9xico, D. F., Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mel\u00e9ndez-Alafort", 
            "givenName": "L.", 
            "id": "sg:person.01021446333.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021446333.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Havana", 
              "id": "https://www.grid.ac/institutes/grid.412165.5", 
              "name": [
                "Laboratorio de Qu\u00edmica Org\u00e1nica, Facultad de Qu\u00edmica, Universidad de la Habana, Habana, Cuba"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mart\u00ednez-Rivero", 
            "givenName": "O.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n", 
              "id": "https://www.grid.ac/institutes/grid.416850.e", 
              "name": [
                "Departamento de Fisiolog\u00eda de la Nutrici\u00f3n, Instituto Nacional de Nutrici\u00f3n Salvador Zubir\u00e1n, INNSZ, M\u00e9xico, D. F., Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "G\u00f3mez", 
            "givenName": "E.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Nacional de Investigaciones Nucleares", 
              "id": "https://www.grid.ac/institutes/grid.419194.0", 
              "name": [
                "Departamento del Reactor y Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, ININ, M\u00e9xico, D.F., Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ferro-Flores", 
            "givenName": "G.", 
            "id": "sg:person.01320041367.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320041367.19"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1097/00002281-199405000-00011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010681857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00002281-199405000-00011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010681857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00002281-199405000-00011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010681857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.76.9.4350", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011704795"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.11.4025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025743417"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0969-8051(94)90093-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026305364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01899076", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039468812", 
              "https://doi.org/10.1007/bf01899076"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01081522", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046492834", 
              "https://doi.org/10.1007/bf01081522"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01081522", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046492834", 
              "https://doi.org/10.1007/bf01081522"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jo00008a014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055960571"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078691359", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078715036", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1997-06", 
        "datePublishedReg": "1997-06-01", 
        "description": "A new technetium labeling method for immunoglobulin reduced with tris(2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobulina IgG source was assayed for purity and optimum reagent's concentration and incubation times were determined. It was purified by column chromatography and labelled with Sn2+ reduced technetium in the presence of MDP. The kit is easy to prepare, labeling efficiency is >(97\u00b11.9) % and stable for 6 hours. The immunoreactivity of the99mTc-IgG was verified by electrophoresis and Western blot tests. The IgG retained its structure after both the reducing and labeling processes and it was the only labeled species.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf02035345", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1094634", 
            "issn": [
              "0236-5731", 
              "1588-2780"
            ], 
            "name": "Journal of Radioanalytical and Nuclear Chemistry", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "220"
          }
        ], 
        "name": "Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins", 
        "pagination": "41-45", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "22a693190b3ae61312b10527cee41f8da5aa25c32213a7a14319aee6ac5d7efd"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf02035345"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1007274642"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf02035345", 
          "https://app.dimensions.ai/details/publication/pub.1007274642"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T11:31", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000357_0000000357/records_99311_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF02035345"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02035345'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02035345'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02035345'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02035345'


     

    This table displays all metadata directly associated to this object as RDF triples.

    121 TRIPLES      21 PREDICATES      36 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf02035345 schema:about anzsrc-for:03
    2 anzsrc-for:0301
    3 schema:author N4540be38a3534a08845b82faafd36d31
    4 schema:citation sg:pub.10.1007/bf01081522
    5 sg:pub.10.1007/bf01899076
    6 https://app.dimensions.ai/details/publication/pub.1078691359
    7 https://app.dimensions.ai/details/publication/pub.1078715036
    8 https://doi.org/10.1016/0969-8051(94)90093-0
    9 https://doi.org/10.1021/jo00008a014
    10 https://doi.org/10.1073/pnas.76.9.4350
    11 https://doi.org/10.1073/pnas.85.11.4025
    12 https://doi.org/10.1097/00002281-199405000-00011
    13 schema:datePublished 1997-06
    14 schema:datePublishedReg 1997-06-01
    15 schema:description A new technetium labeling method for immunoglobulin reduced with tris(2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobulina IgG source was assayed for purity and optimum reagent's concentration and incubation times were determined. It was purified by column chromatography and labelled with Sn2+ reduced technetium in the presence of MDP. The kit is easy to prepare, labeling efficiency is >(97±1.9) % and stable for 6 hours. The immunoreactivity of the99mTc-IgG was verified by electrophoresis and Western blot tests. The IgG retained its structure after both the reducing and labeling processes and it was the only labeled species.
    16 schema:genre research_article
    17 schema:inLanguage en
    18 schema:isAccessibleForFree false
    19 schema:isPartOf N2e671ae31a564cc2a4427480e0ff9a0d
    20 Nc45428a70ab54a9f8f15ab80c609dab2
    21 sg:journal.1094634
    22 schema:name Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins
    23 schema:pagination 41-45
    24 schema:productId Nc9adba62c67b4dbdae62b5028525969c
    25 Ne2e557badd5841d3a4188ad9e8ee537a
    26 Nf8792d075521473db8ca26f638bee76f
    27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007274642
    28 https://doi.org/10.1007/bf02035345
    29 schema:sdDatePublished 2019-04-11T11:31
    30 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    31 schema:sdPublisher N826efbbdbbdb45d199d0f1ed5b0a0995
    32 schema:url http://link.springer.com/10.1007/BF02035345
    33 sgo:license sg:explorer/license/
    34 sgo:sdDataset articles
    35 rdf:type schema:ScholarlyArticle
    36 N10500c3d70cc403b91ed902ea1da976d rdf:first Ncb435e761e4b43529a8666b9f7c2db3a
    37 rdf:rest Nd054b0545e6f488b8506a966a4c1907e
    38 N2e671ae31a564cc2a4427480e0ff9a0d schema:issueNumber 1
    39 rdf:type schema:PublicationIssue
    40 N36b79a446a164fd8a507c74940e4151b rdf:first N8a352659c97649f8a479266d3b7befeb
    41 rdf:rest N10500c3d70cc403b91ed902ea1da976d
    42 N4540be38a3534a08845b82faafd36d31 rdf:first sg:person.01275074523.41
    43 rdf:rest N5e0c3223ae8e417ba2f445ed8a3f7446
    44 N5e0c3223ae8e417ba2f445ed8a3f7446 rdf:first sg:person.01021446333.88
    45 rdf:rest N36b79a446a164fd8a507c74940e4151b
    46 N826efbbdbbdb45d199d0f1ed5b0a0995 schema:name Springer Nature - SN SciGraph project
    47 rdf:type schema:Organization
    48 N8a352659c97649f8a479266d3b7befeb schema:affiliation https://www.grid.ac/institutes/grid.412165.5
    49 schema:familyName Martínez-Rivero
    50 schema:givenName O.
    51 rdf:type schema:Person
    52 Nc45428a70ab54a9f8f15ab80c609dab2 schema:volumeNumber 220
    53 rdf:type schema:PublicationVolume
    54 Nc9adba62c67b4dbdae62b5028525969c schema:name doi
    55 schema:value 10.1007/bf02035345
    56 rdf:type schema:PropertyValue
    57 Ncb435e761e4b43529a8666b9f7c2db3a schema:affiliation https://www.grid.ac/institutes/grid.416850.e
    58 schema:familyName Gómez
    59 schema:givenName E.
    60 rdf:type schema:Person
    61 Nd054b0545e6f488b8506a966a4c1907e rdf:first sg:person.01320041367.19
    62 rdf:rest rdf:nil
    63 Ne2e557badd5841d3a4188ad9e8ee537a schema:name readcube_id
    64 schema:value 22a693190b3ae61312b10527cee41f8da5aa25c32213a7a14319aee6ac5d7efd
    65 rdf:type schema:PropertyValue
    66 Nf8792d075521473db8ca26f638bee76f schema:name dimensions_id
    67 schema:value pub.1007274642
    68 rdf:type schema:PropertyValue
    69 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    70 schema:name Chemical Sciences
    71 rdf:type schema:DefinedTerm
    72 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
    73 schema:name Analytical Chemistry
    74 rdf:type schema:DefinedTerm
    75 sg:journal.1094634 schema:issn 0236-5731
    76 1588-2780
    77 schema:name Journal of Radioanalytical and Nuclear Chemistry
    78 rdf:type schema:Periodical
    79 sg:person.01021446333.88 schema:affiliation https://www.grid.ac/institutes/grid.416850.e
    80 schema:familyName Meléndez-Alafort
    81 schema:givenName L.
    82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021446333.88
    83 rdf:type schema:Person
    84 sg:person.01275074523.41 schema:affiliation https://www.grid.ac/institutes/grid.416850.e
    85 schema:familyName Arteaga de Murphy
    86 schema:givenName C.
    87 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275074523.41
    88 rdf:type schema:Person
    89 sg:person.01320041367.19 schema:affiliation https://www.grid.ac/institutes/grid.419194.0
    90 schema:familyName Ferro-Flores
    91 schema:givenName G.
    92 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320041367.19
    93 rdf:type schema:Person
    94 sg:pub.10.1007/bf01081522 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046492834
    95 https://doi.org/10.1007/bf01081522
    96 rdf:type schema:CreativeWork
    97 sg:pub.10.1007/bf01899076 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039468812
    98 https://doi.org/10.1007/bf01899076
    99 rdf:type schema:CreativeWork
    100 https://app.dimensions.ai/details/publication/pub.1078691359 schema:CreativeWork
    101 https://app.dimensions.ai/details/publication/pub.1078715036 schema:CreativeWork
    102 https://doi.org/10.1016/0969-8051(94)90093-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026305364
    103 rdf:type schema:CreativeWork
    104 https://doi.org/10.1021/jo00008a014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055960571
    105 rdf:type schema:CreativeWork
    106 https://doi.org/10.1073/pnas.76.9.4350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011704795
    107 rdf:type schema:CreativeWork
    108 https://doi.org/10.1073/pnas.85.11.4025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025743417
    109 rdf:type schema:CreativeWork
    110 https://doi.org/10.1097/00002281-199405000-00011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010681857
    111 rdf:type schema:CreativeWork
    112 https://www.grid.ac/institutes/grid.412165.5 schema:alternateName University of Havana
    113 schema:name Laboratorio de Química Orgánica, Facultad de Química, Universidad de la Habana, Habana, Cuba
    114 rdf:type schema:Organization
    115 https://www.grid.ac/institutes/grid.416850.e schema:alternateName Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
    116 schema:name Departamento de Fisiología de la Nutrición, Instituto Nacional de Nutrición Salvador Zubirán, INNSZ, México, D. F., Mexico
    117 Radiofarmacia, Departamento de Medicina Nuclear, Instituto Nacional de Nutrición Salvador Zubirán, INNSZ, México, D. F., Mexico
    118 rdf:type schema:Organization
    119 https://www.grid.ac/institutes/grid.419194.0 schema:alternateName Instituto Nacional de Investigaciones Nucleares
    120 schema:name Departamento del Reactor y Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, ININ, México, D.F., Mexico
    121 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...