Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-06

AUTHORS

C. Arteaga de Murphy, L. Meléndez-Alafort, O. Martínez-Rivero, E. Gómez, G. Ferro-Flores

ABSTRACT

A new technetium labeling method for immunoglobulin reduced with tris(2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobulina IgG source was assayed for purity and optimum reagent's concentration and incubation times were determined. It was purified by column chromatography and labelled with Sn2+ reduced technetium in the presence of MDP. The kit is easy to prepare, labeling efficiency is >(97±1.9) % and stable for 6 hours. The immunoreactivity of the99mTc-IgG was verified by electrophoresis and Western blot tests. The IgG retained its structure after both the reducing and labeling processes and it was the only labeled species. More... »

PAGES

41-45

References to SciGraph publications

  • 1969-02. Reduction of biological substances by water-soluble phosphines: Gamma-globulin (IgG) in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 1995-03. Radiolabeling with technetium-99m to study high-capacity and low-capacity biochemical systems in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02035345

    DOI

    http://dx.doi.org/10.1007/bf02035345

    DIMENSIONS

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