Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-04

AUTHORS

J. de Jongh-Leuvenink, A. S. Bouter, J. H. Marcelis, J. Schellekens, J. Verhoef

ABSTRACT

Monoclonal antibodies were produced against Escherichia coli O111, Escherichia coli J5, and the rough (R) mutant of Salmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay. Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gram-negative bacteria are not similar. More... »

PAGES

148-151

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02013970

DOI

http://dx.doi.org/10.1007/bf02013970

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016178733

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2424754


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