Active uptake of sodium in the gills of the hyperregulating shore crabCarcinus maenas View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1986-03

AUTHORS

D. Siebers, Č. Lucu, A. Winkler, L. Dalla Venezia, H. Wille

ABSTRACT

Isolated posterior gills of shore crabs,Carcinus maenas, previously acclimated for at least 1 month to brackish water of 10 ‰ S, were connected with an artificial hemolymph circulation by means of thin polyethylene tubings. Gills were symmetrically perfused and bathed with 50 % sea water. Transepithelial potential differences (PDs) and fluxes of sodium between medium and blood were measured under control conditions and following reductions of PDs by means of 5 mM internal (blood side) ouabain, 0.5 mM internal and external (bathing medium) NaCN or by exhaustion of energy reserves along with a prolonged perfusion period of more than 9 h. In these experiments22Na was used as tracer. Each of the three modes of reducing transepithelial potential differences resulted in a decrease in sodium influxes from 500–1000 µmoles g−1 h−1 to 250–400 µmoles g−1 h−1. The findings suggest that sodium influx, which normally greatly exceeds efflux, was diminished by its active component. The remaining non-inhibitable influx equals efflux values. Our findings thus indicate that efflux is completely passive, while influx has — beside a passive component of efflux magnitudes — an additional active portion which is much larger than the passive component. Since ouabain is a specific inhibitor of the Na-K-ATPase, our results confirm previous findings (Siebers et al., 1985) that the basolaterally located Na-K-ATPase generates the transepithelial potential difference in the gills, which is inside negative by about 6–12 mV. Inhibition of the active portion of sodium influx by internal ouabain along with reduced PDs suggests that transepithelial PDs generated by the branchial sodium pump are the driving force for active sodium uptake in hyperregulating brackish water crabs. More... »

PAGES

151-160

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01987292

DOI

http://dx.doi.org/10.1007/bf01987292

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006764820


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