Liposome-mediated gene transfer in fish embryos View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-03

AUTHORS

József Szelei, László Váradi, Ferenc Müller, Ferenc Erdélyi, László Orbán, László Horváth, Ernő Duda

ABSTRACT

Liposome-mediated gene transfer was used to introduce large DNA constructs into zygotes of African catfish. The technique is based on the delivery of recombinant bacteriophage lambda particles (or DNA-protamine complexes) into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Dechorionated zygotes and early fish embryos were treated with the transforming liposomes. Expression of the introduced reporter genes during the first three weeks of the development of the larvae was followed by measuring the activity of corresponding enzymes. These assays have indicated very efficient DNA uptake into the embryos. More... »

PAGES

116-119

Journal

TITLE

Transgenic Research

ISSUE

2

VOLUME

3

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01974089

DOI

http://dx.doi.org/10.1007/bf01974089

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013614803


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.481814.0", 
          "name": [
            "Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Szelei", 
        "givenName": "J\u00f3zsef", 
        "id": "sg:person.01104065143.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104065143.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.129553.9", 
          "name": [
            "Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "V\u00e1radi", 
        "givenName": "L\u00e1szl\u00f3", 
        "id": "sg:person.01177441513.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177441513.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
            "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "M\u00fcller", 
        "givenName": "Ferenc", 
        "id": "sg:person.0705164240.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705164240.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Erd\u00e9lyi", 
        "givenName": "Ferenc", 
        "id": "sg:person.01312521504.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312521504.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Orb\u00e1n", 
        "givenName": "L\u00e1szl\u00f3", 
        "id": "sg:person.0755740070.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755740070.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, G\u00f6d\u00f6ll\u0151, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.129553.9", 
          "name": [
            "Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Horv\u00e1th", 
        "givenName": "L\u00e1szl\u00f3", 
        "id": "sg:person.012262476147.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012262476147.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary", 
          "id": "http://www.grid.ac/institutes/grid.481814.0", 
          "name": [
            "Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Duda", 
        "givenName": "Ern\u0151", 
        "id": "sg:person.01313717561.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313717561.84"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-1-4615-3760-1_15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014453081", 
          "https://doi.org/10.1007/978-1-4615-3760-1_15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01972609", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010197648", 
          "https://doi.org/10.1007/bf01972609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00652482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006342460", 
          "https://doi.org/10.1007/bf00652482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00265299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047907130", 
          "https://doi.org/10.1007/bf00265299"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1994-03", 
    "datePublishedReg": "1994-03-01", 
    "description": "Liposome-mediated gene transfer was used to introduce large DNA constructs into zygotes of African catfish. The technique is based on the delivery of recombinant bacteriophage lambda particles (or DNA-protamine complexes) into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Dechorionated zygotes and early fish embryos were treated with the transforming liposomes. Expression of the introduced reporter genes during the first three weeks of the development of the larvae was followed by measuring the activity of corresponding enzymes. These assays have indicated very efficient DNA uptake into the embryos.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf01974089", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1102526", 
        "issn": [
          "0962-8819", 
          "1573-9368"
        ], 
        "name": "Transgenic Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "fish embryos", 
      "gene transfer", 
      "early fish embryos", 
      "large DNA constructs", 
      "efficient DNA uptake", 
      "DNA uptake", 
      "bacteriophage lambda particles", 
      "corresponding enzymes", 
      "reporter gene", 
      "DNA constructs", 
      "embryos", 
      "zygotes", 
      "lambda particles", 
      "target cells", 
      "liposomes", 
      "African catfish", 
      "genes", 
      "larvae", 
      "cytoplasm", 
      "enzyme", 
      "catfish", 
      "large unilamellar liposomes", 
      "expression", 
      "cells", 
      "assays", 
      "unilamellar liposomes", 
      "uptake", 
      "delivery", 
      "particles", 
      "transfer", 
      "activity", 
      "constructs", 
      "technique", 
      "development", 
      "weeks"
    ], 
    "name": "Liposome-mediated gene transfer in fish embryos", 
    "pagination": "116-119", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013614803"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01974089"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01974089", 
      "https://app.dimensions.ai/details/publication/pub.1013614803"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-10T09:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_226.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf01974089"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01974089'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01974089'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01974089'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01974089'


 

This table displays all metadata directly associated to this object as RDF triples.

158 TRIPLES      22 PREDICATES      65 URIs      53 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01974089 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nf5102fca07b2400cb062926574d39f33
4 schema:citation sg:pub.10.1007/978-1-4615-3760-1_15
5 sg:pub.10.1007/bf00265299
6 sg:pub.10.1007/bf00652482
7 sg:pub.10.1007/bf01972609
8 schema:datePublished 1994-03
9 schema:datePublishedReg 1994-03-01
10 schema:description Liposome-mediated gene transfer was used to introduce large DNA constructs into zygotes of African catfish. The technique is based on the delivery of recombinant bacteriophage lambda particles (or DNA-protamine complexes) into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Dechorionated zygotes and early fish embryos were treated with the transforming liposomes. Expression of the introduced reporter genes during the first three weeks of the development of the larvae was followed by measuring the activity of corresponding enzymes. These assays have indicated very efficient DNA uptake into the embryos.
11 schema:genre article
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N3195a19c69714fd8a8726708203d0c2b
15 Nf32f06b266d94c24ae9808d4c6f4933e
16 sg:journal.1102526
17 schema:keywords African catfish
18 DNA constructs
19 DNA uptake
20 activity
21 assays
22 bacteriophage lambda particles
23 catfish
24 cells
25 constructs
26 corresponding enzymes
27 cytoplasm
28 delivery
29 development
30 early fish embryos
31 efficient DNA uptake
32 embryos
33 enzyme
34 expression
35 fish embryos
36 gene transfer
37 genes
38 lambda particles
39 large DNA constructs
40 large unilamellar liposomes
41 larvae
42 liposomes
43 particles
44 reporter gene
45 target cells
46 technique
47 transfer
48 unilamellar liposomes
49 uptake
50 weeks
51 zygotes
52 schema:name Liposome-mediated gene transfer in fish embryos
53 schema:pagination 116-119
54 schema:productId N3eedcf6eefc04f6bb474d674e64566b9
55 Nb2484f06d36a417f8b0654a8b9632dae
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013614803
57 https://doi.org/10.1007/bf01974089
58 schema:sdDatePublished 2022-05-10T09:41
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher N83df37fd30334445884a8af8b1922e06
61 schema:url https://doi.org/10.1007/bf01974089
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N315a9eb7f1ae4fbeab5266ec14e4f1ef rdf:first sg:person.0755740070.52
66 rdf:rest Na0b6cd55f4c14793a5e5759c5aaa68e0
67 N3195a19c69714fd8a8726708203d0c2b schema:volumeNumber 3
68 rdf:type schema:PublicationVolume
69 N3eedcf6eefc04f6bb474d674e64566b9 schema:name doi
70 schema:value 10.1007/bf01974089
71 rdf:type schema:PropertyValue
72 N4926c926a5e54c6c84d38d97110226a0 rdf:first sg:person.0705164240.38
73 rdf:rest N877c29ec66854d69822dbb1a4392a00b
74 N83df37fd30334445884a8af8b1922e06 schema:name Springer Nature - SN SciGraph project
75 rdf:type schema:Organization
76 N877c29ec66854d69822dbb1a4392a00b rdf:first sg:person.01312521504.32
77 rdf:rest N315a9eb7f1ae4fbeab5266ec14e4f1ef
78 Na0b6cd55f4c14793a5e5759c5aaa68e0 rdf:first sg:person.012262476147.78
79 rdf:rest Nb162d3463b444bc1955472a062065aa9
80 Nb162d3463b444bc1955472a062065aa9 rdf:first sg:person.01313717561.84
81 rdf:rest rdf:nil
82 Nb2484f06d36a417f8b0654a8b9632dae schema:name dimensions_id
83 schema:value pub.1013614803
84 rdf:type schema:PropertyValue
85 Nb969859079a4447fa8fca935b30e0d85 rdf:first sg:person.01177441513.04
86 rdf:rest N4926c926a5e54c6c84d38d97110226a0
87 Nf32f06b266d94c24ae9808d4c6f4933e schema:issueNumber 2
88 rdf:type schema:PublicationIssue
89 Nf5102fca07b2400cb062926574d39f33 rdf:first sg:person.01104065143.85
90 rdf:rest Nb969859079a4447fa8fca935b30e0d85
91 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
92 schema:name Biological Sciences
93 rdf:type schema:DefinedTerm
94 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
95 schema:name Genetics
96 rdf:type schema:DefinedTerm
97 sg:journal.1102526 schema:issn 0962-8819
98 1573-9368
99 schema:name Transgenic Research
100 schema:publisher Springer Nature
101 rdf:type schema:Periodical
102 sg:person.01104065143.85 schema:affiliation grid-institutes:grid.481814.0
103 schema:familyName Szelei
104 schema:givenName József
105 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104065143.85
106 rdf:type schema:Person
107 sg:person.01177441513.04 schema:affiliation grid-institutes:grid.129553.9
108 schema:familyName Váradi
109 schema:givenName László
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177441513.04
111 rdf:type schema:Person
112 sg:person.012262476147.78 schema:affiliation grid-institutes:grid.129553.9
113 schema:familyName Horváth
114 schema:givenName László
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012262476147.78
116 rdf:type schema:Person
117 sg:person.01312521504.32 schema:affiliation grid-institutes:grid.417744.5
118 schema:familyName Erdélyi
119 schema:givenName Ferenc
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312521504.32
121 rdf:type schema:Person
122 sg:person.01313717561.84 schema:affiliation grid-institutes:grid.481814.0
123 schema:familyName Duda
124 schema:givenName Ernő
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313717561.84
126 rdf:type schema:Person
127 sg:person.0705164240.38 schema:affiliation grid-institutes:grid.417744.5
128 schema:familyName Müller
129 schema:givenName Ferenc
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705164240.38
131 rdf:type schema:Person
132 sg:person.0755740070.52 schema:affiliation grid-institutes:grid.417744.5
133 schema:familyName Orbán
134 schema:givenName László
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755740070.52
136 rdf:type schema:Person
137 sg:pub.10.1007/978-1-4615-3760-1_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014453081
138 https://doi.org/10.1007/978-1-4615-3760-1_15
139 rdf:type schema:CreativeWork
140 sg:pub.10.1007/bf00265299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047907130
141 https://doi.org/10.1007/bf00265299
142 rdf:type schema:CreativeWork
143 sg:pub.10.1007/bf00652482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006342460
144 https://doi.org/10.1007/bf00652482
145 rdf:type schema:CreativeWork
146 sg:pub.10.1007/bf01972609 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010197648
147 https://doi.org/10.1007/bf01972609
148 rdf:type schema:CreativeWork
149 grid-institutes:grid.129553.9 schema:alternateName Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, Gödöllő, Hungary
150 schema:name Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, Gödöllő, Hungary
151 rdf:type schema:Organization
152 grid-institutes:grid.417744.5 schema:alternateName Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, Gödöllő, Hungary
153 schema:name Institute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, Gödöllő, Hungary
154 Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, H-2101, Gödöllő, Hungary
155 rdf:type schema:Organization
156 grid-institutes:grid.481814.0 schema:alternateName Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary
157 schema:name Institute for Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6726, Szeged, Hungary
158 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...