Variability of organ-specific gene expression in transgenic tobacco plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-05

AUTHORS

Cornelia van der Hoeven, Antje Dietz, Jorg Landsmann

ABSTRACT

Variability of expression of introduced marker genes was analysed in a large number of tobacco regenerants from anAgrobacterium-mediated transformation. In spite of standardization of sampling, considerable variation of GUS and NPTII expression was observed between individual transformants at different times of analysis and in different parts of the same plant. Organ-specificity of root versus leaf expression conferred by the par promoter from the haemoglobin gene ofParasponia andersonii in front of thegus gene showed a continuous spectrum. GUS expression in roots was found in 128 out of 140 plants; expression in leaves was found in 46 plants, and was always lower than in the corresponding roots. NPTII expression regulated by the nos promoter also showed a continuous spectrum. Expression levels were generally higher in roots than in leaves. Plants with high GUS expression in leaves showed high NPTII activity as well. A positive correlation between the level of NPTII expression and the numbers of integrated gene copies was noted. Chromosomal position effects and physiological determination are suggested as triggers for the variations. The transformed regenerated tobacco plants were largely comparable to clonal variants. More... »

PAGES

159-166

References to SciGraph publications

  • 1991-12. The stability of foreign protein production in genetically modified plant cells in PLANT CELL REPORTS
  • 1992-02. Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic petunia and its colour phenotype in MOLECULAR GENETICS AND GENOMICS
  • 1991-07. Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters in PLANT MOLECULAR BIOLOGY
  • 1990-04. Quantitation of chloramphenicol acetyl transferase in transgenic tobacco plants by ELISA and correlation with gene copy number in PLANT MOLECULAR BIOLOGY
  • 1988-09. Organ regulated expression of the Parasponia andersonii haemoglobin gene in transgenic tobacco plants in MOLECULAR GENETICS AND GENOMICS
  • 1987-05. T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1987-05. T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1990-12. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants in PLANT MOLECULAR BIOLOGY
  • 1993-01. Transgene copy number can be positively or negatively associated with transgene expression in PLANT MOLECULAR BIOLOGY
  • 1986-11. Common evolutionary origin of legume and non-legume plant haemoglobins in NATURE
  • 1990-01. Expression of a rice glutelin promoter in transgenic tobacco in PLANT MOLECULAR BIOLOGY
  • 1986-10. T-DNA structure in transgenic tobacco plants with multiple independent integration sites in MOLECULAR GENETICS AND GENOMICS
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1990-07. Organ-specific modulation of gene expression in transgenic plants using antisene RNA in PLANT MOLECULAR BIOLOGY
  • 1985-12. Somaclonal variation in Solanum tuberosum detected at the molecular level in THEORETICAL AND APPLIED GENETICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988-05. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1988-05. Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1992-12. Translation controls the expression level of a chimaeric reporter gene in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01973983

    DOI

    http://dx.doi.org/10.1007/bf01973983

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012315006


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Braunschweig University of Technology", 
              "id": "https://www.grid.ac/institutes/grid.6738.a", 
              "name": [
                "Biologische Bundesanstalt f\u00fcr Land- und Forstwirtschaft, Institut f\u00fcr Biochemie und Pflanzenvirologie, Messeweg 11/12, D-38104, Braunschweig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van der Hoeven", 
            "givenName": "Cornelia", 
            "id": "sg:person.0612247040.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612247040.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Braunschweig University of Technology", 
              "id": "https://www.grid.ac/institutes/grid.6738.a", 
              "name": [
                "Biologische Bundesanstalt f\u00fcr Land- und Forstwirtschaft, Institut f\u00fcr Biochemie und Pflanzenvirologie, Messeweg 11/12, D-38104, Braunschweig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dietz", 
            "givenName": "Antje", 
            "id": "sg:person.01066257402.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066257402.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Braunschweig University of Technology", 
              "id": "https://www.grid.ac/institutes/grid.6738.a", 
              "name": [
                "Biologische Bundesanstalt f\u00fcr Land- und Forstwirtschaft, Institut f\u00fcr Biochemie und Pflanzenvirologie, Messeweg 11/12, D-38104, Braunschweig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Landsmann", 
            "givenName": "Jorg", 
            "id": "sg:person.0726475440.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726475440.82"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00036805", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000421689", 
              "https://doi.org/10.1007/bf00036805"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00036805", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000421689", 
              "https://doi.org/10.1007/bf00036805"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.81.1.86", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000555249"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/13.19.6981", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006386102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/324166a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007645912", 
              "https://doi.org/10.1038/324166a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027392", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010365050", 
              "https://doi.org/10.1007/bf00027392"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027392", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010365050", 
              "https://doi.org/10.1007/bf00027392"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.2.7.633", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012771082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.88.3.547", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015681026"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.ge.20.120186.002341", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016764856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/303179a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018421874", 
              "https://doi.org/10.1038/303179a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00015653", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019337918", 
              "https://doi.org/10.1007/bf00015653"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00015653", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019337918", 
              "https://doi.org/10.1007/bf00015653"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/16.15.7601", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019876717"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331617", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022012805", 
              "https://doi.org/10.1007/bf00331617"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.86.4.1281", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022583006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00017722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023325980", 
              "https://doi.org/10.1007/bf00017722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00017722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023325980", 
              "https://doi.org/10.1007/bf00017722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.76.4.1648", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025361704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02428030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026692189", 
              "https://doi.org/10.1007/bf02428030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02428030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026692189", 
              "https://doi.org/10.1007/bf02428030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026960131", 
              "https://doi.org/10.1007/bf00027393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026960131", 
              "https://doi.org/10.1007/bf00027393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039425", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029834259", 
              "https://doi.org/10.1007/bf00039425"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039425", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029834259", 
              "https://doi.org/10.1007/bf00039425"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039614", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033165259", 
              "https://doi.org/10.1007/bf00039614"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039614", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033165259", 
              "https://doi.org/10.1007/bf00039614"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00292701", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034591291", 
              "https://doi.org/10.1007/bf00292701"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035032654", 
              "https://doi.org/10.1007/bf00027163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035032654", 
              "https://doi.org/10.1007/bf00027163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027503", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035398678", 
              "https://doi.org/10.1007/bf00027503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027503", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035398678", 
              "https://doi.org/10.1007/bf00027503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00633837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040244648", 
              "https://doi.org/10.1007/bf00633837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00633837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040244648", 
              "https://doi.org/10.1007/bf00633837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331618", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041238099", 
              "https://doi.org/10.1007/bf00331618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00340181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041630168", 
              "https://doi.org/10.1007/bf00340181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/15.8.3479", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044575618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0168-9452(89)90121-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046326870"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.83.22.8447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047987843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/15.4.1543", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048663102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00251196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049899285", 
              "https://doi.org/10.1007/bf00251196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00251196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049899285", 
              "https://doi.org/10.1007/bf00251196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00234589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052233294", 
              "https://doi.org/10.1007/bf00234589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00234589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052233294", 
              "https://doi.org/10.1007/bf00234589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/16.19.9267", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059931705"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.227.4691.1229", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062529900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.234.4778.856", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062533325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03949.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077060020"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1989.tb03421.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077184027"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1989.tb03383.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079178806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079687101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/003072709001900207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090557441"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/003072709001900207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090557441"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-05", 
        "datePublishedReg": "1994-05-01", 
        "description": "Variability of expression of introduced marker genes was analysed in a large number of tobacco regenerants from anAgrobacterium-mediated transformation. In spite of standardization of sampling, considerable variation of GUS and NPTII expression was observed between individual transformants at different times of analysis and in different parts of the same plant. Organ-specificity of root versus leaf expression conferred by the par promoter from the haemoglobin gene ofParasponia andersonii in front of thegus gene showed a continuous spectrum. GUS expression in roots was found in 128 out of 140 plants; expression in leaves was found in 46 plants, and was always lower than in the corresponding roots. NPTII expression regulated by the nos promoter also showed a continuous spectrum. Expression levels were generally higher in roots than in leaves. Plants with high GUS expression in leaves showed high NPTII activity as well. A positive correlation between the level of NPTII expression and the numbers of integrated gene copies was noted. Chromosomal position effects and physiological determination are suggested as triggers for the variations. The transformed regenerated tobacco plants were largely comparable to clonal variants.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf01973983", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1102526", 
            "issn": [
              "0962-8819", 
              "1573-9368"
            ], 
            "name": "Transgenic Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "3"
          }
        ], 
        "name": "Variability of organ-specific gene expression in transgenic tobacco plants", 
        "pagination": "159-166", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "3aac19de4832616913e3bae9a5e9d976d5f5944fd194d51b87e823adb0ee1d69"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf01973983"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1012315006"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf01973983", 
          "https://app.dimensions.ai/details/publication/pub.1012315006"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T14:06", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000498.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF01973983"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01973983'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01973983'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01973983'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01973983'


     

    This table displays all metadata directly associated to this object as RDF triples.

    224 TRIPLES      21 PREDICATES      70 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf01973983 schema:about anzsrc-for:06
    2 anzsrc-for:0607
    3 schema:author N7e7addb332b1457da612d83647f77422
    4 schema:citation sg:pub.10.1007/bf00015653
    5 sg:pub.10.1007/bf00017722
    6 sg:pub.10.1007/bf00027163
    7 sg:pub.10.1007/bf00027392
    8 sg:pub.10.1007/bf00027393
    9 sg:pub.10.1007/bf00027503
    10 sg:pub.10.1007/bf00036805
    11 sg:pub.10.1007/bf00039425
    12 sg:pub.10.1007/bf00039614
    13 sg:pub.10.1007/bf00234589
    14 sg:pub.10.1007/bf00251196
    15 sg:pub.10.1007/bf00292701
    16 sg:pub.10.1007/bf00331617
    17 sg:pub.10.1007/bf00331618
    18 sg:pub.10.1007/bf00340181
    19 sg:pub.10.1007/bf00633837
    20 sg:pub.10.1007/bf02428030
    21 sg:pub.10.1007/bf02712670
    22 sg:pub.10.1038/303179a0
    23 sg:pub.10.1038/324166a0
    24 https://doi.org/10.1002/j.1460-2075.1985.tb03949.x
    25 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
    26 https://doi.org/10.1002/j.1460-2075.1989.tb03383.x
    27 https://doi.org/10.1002/j.1460-2075.1989.tb03421.x
    28 https://doi.org/10.1016/0168-9452(89)90121-0
    29 https://doi.org/10.1016/0378-1119(85)90120-9
    30 https://doi.org/10.1016/s0022-2836(75)80083-0
    31 https://doi.org/10.1073/pnas.76.4.1648
    32 https://doi.org/10.1073/pnas.83.22.8447
    33 https://doi.org/10.1093/nar/13.19.6981
    34 https://doi.org/10.1093/nar/15.4.1543
    35 https://doi.org/10.1093/nar/15.8.3479
    36 https://doi.org/10.1093/nar/16.15.7601
    37 https://doi.org/10.1093/nar/16.19.9267
    38 https://doi.org/10.1104/pp.81.1.86
    39 https://doi.org/10.1104/pp.86.4.1281
    40 https://doi.org/10.1104/pp.88.3.547
    41 https://doi.org/10.1105/tpc.2.7.633
    42 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    43 https://doi.org/10.1126/science.227.4691.1229
    44 https://doi.org/10.1126/science.234.4778.856
    45 https://doi.org/10.1146/annurev.ge.20.120186.002341
    46 https://doi.org/10.1177/003072709001900207
    47 schema:datePublished 1994-05
    48 schema:datePublishedReg 1994-05-01
    49 schema:description Variability of expression of introduced marker genes was analysed in a large number of tobacco regenerants from anAgrobacterium-mediated transformation. In spite of standardization of sampling, considerable variation of GUS and NPTII expression was observed between individual transformants at different times of analysis and in different parts of the same plant. Organ-specificity of root versus leaf expression conferred by the par promoter from the haemoglobin gene ofParasponia andersonii in front of thegus gene showed a continuous spectrum. GUS expression in roots was found in 128 out of 140 plants; expression in leaves was found in 46 plants, and was always lower than in the corresponding roots. NPTII expression regulated by the nos promoter also showed a continuous spectrum. Expression levels were generally higher in roots than in leaves. Plants with high GUS expression in leaves showed high NPTII activity as well. A positive correlation between the level of NPTII expression and the numbers of integrated gene copies was noted. Chromosomal position effects and physiological determination are suggested as triggers for the variations. The transformed regenerated tobacco plants were largely comparable to clonal variants.
    50 schema:genre research_article
    51 schema:inLanguage en
    52 schema:isAccessibleForFree false
    53 schema:isPartOf N017d895753944ef0b4e56d7a03d84e5a
    54 N1dd7b4245d594fbda443df0c2996c575
    55 sg:journal.1102526
    56 schema:name Variability of organ-specific gene expression in transgenic tobacco plants
    57 schema:pagination 159-166
    58 schema:productId N2293d79313974eac9f1553b6deca8775
    59 Nad353e1a4e8e4047ba0874174436d341
    60 Nfdc92f5fc97e4181af4dd401c480671a
    61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012315006
    62 https://doi.org/10.1007/bf01973983
    63 schema:sdDatePublished 2019-04-10T14:06
    64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    65 schema:sdPublisher Ne992e60076214e71b6e5943904c705bf
    66 schema:url http://link.springer.com/10.1007/BF01973983
    67 sgo:license sg:explorer/license/
    68 sgo:sdDataset articles
    69 rdf:type schema:ScholarlyArticle
    70 N017d895753944ef0b4e56d7a03d84e5a schema:volumeNumber 3
    71 rdf:type schema:PublicationVolume
    72 N1dd7b4245d594fbda443df0c2996c575 schema:issueNumber 3
    73 rdf:type schema:PublicationIssue
    74 N2293d79313974eac9f1553b6deca8775 schema:name dimensions_id
    75 schema:value pub.1012315006
    76 rdf:type schema:PropertyValue
    77 N7e7addb332b1457da612d83647f77422 rdf:first sg:person.0612247040.32
    78 rdf:rest Na3ff80630dd142c5abf6adf20d535d38
    79 Na3ff80630dd142c5abf6adf20d535d38 rdf:first sg:person.01066257402.60
    80 rdf:rest Naed3977c4109493ca5ed8e5d70925039
    81 Nad353e1a4e8e4047ba0874174436d341 schema:name readcube_id
    82 schema:value 3aac19de4832616913e3bae9a5e9d976d5f5944fd194d51b87e823adb0ee1d69
    83 rdf:type schema:PropertyValue
    84 Naed3977c4109493ca5ed8e5d70925039 rdf:first sg:person.0726475440.82
    85 rdf:rest rdf:nil
    86 Ne992e60076214e71b6e5943904c705bf schema:name Springer Nature - SN SciGraph project
    87 rdf:type schema:Organization
    88 Nfdc92f5fc97e4181af4dd401c480671a schema:name doi
    89 schema:value 10.1007/bf01973983
    90 rdf:type schema:PropertyValue
    91 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    92 schema:name Biological Sciences
    93 rdf:type schema:DefinedTerm
    94 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    95 schema:name Plant Biology
    96 rdf:type schema:DefinedTerm
    97 sg:journal.1102526 schema:issn 0962-8819
    98 1573-9368
    99 schema:name Transgenic Research
    100 rdf:type schema:Periodical
    101 sg:person.01066257402.60 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
    102 schema:familyName Dietz
    103 schema:givenName Antje
    104 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066257402.60
    105 rdf:type schema:Person
    106 sg:person.0612247040.32 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
    107 schema:familyName van der Hoeven
    108 schema:givenName Cornelia
    109 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612247040.32
    110 rdf:type schema:Person
    111 sg:person.0726475440.82 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
    112 schema:familyName Landsmann
    113 schema:givenName Jorg
    114 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726475440.82
    115 rdf:type schema:Person
    116 sg:pub.10.1007/bf00015653 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019337918
    117 https://doi.org/10.1007/bf00015653
    118 rdf:type schema:CreativeWork
    119 sg:pub.10.1007/bf00017722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023325980
    120 https://doi.org/10.1007/bf00017722
    121 rdf:type schema:CreativeWork
    122 sg:pub.10.1007/bf00027163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035032654
    123 https://doi.org/10.1007/bf00027163
    124 rdf:type schema:CreativeWork
    125 sg:pub.10.1007/bf00027392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010365050
    126 https://doi.org/10.1007/bf00027392
    127 rdf:type schema:CreativeWork
    128 sg:pub.10.1007/bf00027393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026960131
    129 https://doi.org/10.1007/bf00027393
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/bf00027503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035398678
    132 https://doi.org/10.1007/bf00027503
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/bf00036805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000421689
    135 https://doi.org/10.1007/bf00036805
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/bf00039425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029834259
    138 https://doi.org/10.1007/bf00039425
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1007/bf00039614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033165259
    141 https://doi.org/10.1007/bf00039614
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/bf00234589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052233294
    144 https://doi.org/10.1007/bf00234589
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/bf00251196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049899285
    147 https://doi.org/10.1007/bf00251196
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/bf00292701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034591291
    150 https://doi.org/10.1007/bf00292701
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1007/bf00331617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022012805
    153 https://doi.org/10.1007/bf00331617
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1007/bf00331618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041238099
    156 https://doi.org/10.1007/bf00331618
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1007/bf00340181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041630168
    159 https://doi.org/10.1007/bf00340181
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1007/bf00633837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040244648
    162 https://doi.org/10.1007/bf00633837
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1007/bf02428030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026692189
    165 https://doi.org/10.1007/bf02428030
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1007/bf02712670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131267
    168 https://doi.org/10.1007/bf02712670
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1038/303179a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
    171 https://doi.org/10.1038/303179a0
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1038/324166a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007645912
    174 https://doi.org/10.1038/324166a0
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1002/j.1460-2075.1985.tb03949.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077060020
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079687101
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1002/j.1460-2075.1989.tb03383.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079178806
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1002/j.1460-2075.1989.tb03421.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077184027
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1016/0168-9452(89)90121-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046326870
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1016/0378-1119(85)90120-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017120147
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1073/pnas.76.4.1648 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025361704
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1073/pnas.83.22.8447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047987843
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1093/nar/13.19.6981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006386102
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1093/nar/15.4.1543 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048663102
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1093/nar/15.8.3479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044575618
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1093/nar/16.15.7601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019876717
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1093/nar/16.19.9267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059931705
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1104/pp.81.1.86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000555249
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1104/pp.86.4.1281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022583006
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1104/pp.88.3.547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015681026
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1105/tpc.2.7.633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012771082
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1126/science.227.4691.1229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062529900
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1126/science.234.4778.856 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062533325
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1146/annurev.ge.20.120186.002341 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016764856
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1177/003072709001900207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090557441
    221 rdf:type schema:CreativeWork
    222 https://www.grid.ac/institutes/grid.6738.a schema:alternateName Braunschweig University of Technology
    223 schema:name Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Messeweg 11/12, D-38104, Braunschweig, Germany
    224 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...