Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-05

AUTHORS

Alan H. Christensen, Peter H. Quail

ABSTRACT

A set of plasmids has been constructed utilizing the promoter, 5′ untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-β-glucuronidase (Ubi-GUS), and ubiquitin-phosphinothricin acetyl transferase (Ubi-bar) have been generated, as well as a construct containing chimaeric genes for bothUbi-GUS andUbi-bar in a single plasmid. Another construct was generated to allow cloning of protein coding sequences of choice onBam HI andBam HI-compatible restriction fragments downstream of theUbi-1 gene fragment. Because theUbi-1 promotor has been shown to be highly active in monocots, these constructs may be useful for generating high-level gene expression of selectable markers to facilitate efficient transformation of monocots, to drive expression of reference reporter genes in studies of gene expression, and to provide expression of biotechnologically important protein products in transgenic plants. More... »

PAGES

213-218

References to SciGraph publications

  • 1990-09-01. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants in BIO/TECHNOLOGY
  • 1993-12-01. Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos in NATURE BIOTECHNOLOGY
  • 1993-10. Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment in PLANT CELL REPORTS
  • 1994-07. Quantitative transient gene expression: Comparison of the promoters for maize polyubiquitin1, rice actin1, maize-derivedEmu andCaMV 35S in cells of barley, maize and tobacco in TRANSGENIC RESEARCH
  • 1993-07. Enhanced GUS gene expression in cereal/grass cell suspensions and immature embryos using the maize uhiquitin-based plasmid pAHC25 in PLANT CELL REPORTS
  • 1993-07-01. Bialaphos Treatment of Transgenic Rice Plants Expressing a bar Gene Prevents Infection by the Sheath Blight Pathogen (Rhizoctonia solani) in NATURE BIOTECHNOLOGY
  • 1994-11. Non-systemic expression of a stress-responsive maize polyubiquitin gene (Ubi-1) in transgenic rice plants in PLANT MOLECULAR BIOLOGY
  • 1993-11. Activity of a maize ubiquitin promoter in transgenic rice in PLANT MOLECULAR BIOLOGY
  • 1995-08. Activity of constitutive promoters in various species from the Liliaceae in PLANT MOLECULAR BIOLOGY
  • 1992-02. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation in PLANT MOLECULAR BIOLOGY
  • 1991-08. Anaerobic induction and tissue-specific expression of maize Adh1 promoter in transgenic rice plants and their progeny in MOLECULAR GENETICS AND GENOMICS
  • Journal

    TITLE

    Transgenic Research

    ISSUE

    3

    VOLUME

    5

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01969712

    DOI

    http://dx.doi.org/10.1007/bf01969712

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002431148

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8673150


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