Ontology type: schema:ScholarlyArticle
1996-05
AUTHORSAlan H. Christensen, Peter H. Quail
ABSTRACTA set of plasmids has been constructed utilizing the promoter, 5′ untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-β-glucuronidase (Ubi-GUS), and ubiquitin-phosphinothricin acetyl transferase (Ubi-bar) have been generated, as well as a construct containing chimaeric genes for bothUbi-GUS andUbi-bar in a single plasmid. Another construct was generated to allow cloning of protein coding sequences of choice onBam HI andBam HI-compatible restriction fragments downstream of theUbi-1 gene fragment. Because theUbi-1 promotor has been shown to be highly active in monocots, these constructs may be useful for generating high-level gene expression of selectable markers to facilitate efficient transformation of monocots, to drive expression of reference reporter genes in studies of gene expression, and to provide expression of biotechnologically important protein products in transgenic plants. More... »
PAGES213-218
http://scigraph.springernature.com/pub.10.1007/bf01969712
DOIhttp://dx.doi.org/10.1007/bf01969712
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1002431148
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8673150
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA, Plant",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation, Plant",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Markers",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Vectors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Genetically Modified",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Promoter Regions, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Fusion Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Selection, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ubiquitins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Zea mays",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "USDA/ARS Plant Gene Expression Center, 800 Buchanan St., 94710, Albany, CA, USA",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Dept. of Plant Biology, University of California, 94720, Berkeley, CA, USA",
"USDA/ARS Plant Gene Expression Center, 800 Buchanan St., 94710, Albany, CA, USA"
],
"type": "Organization"
},
"familyName": "Christensen",
"givenName": "Alan H.",
"id": "sg:person.01300011476.97",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300011476.97"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "USDA/ARS Plant Gene Expression Center, 800 Buchanan St., 94710, Albany, CA, USA",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Dept. of Plant Biology, University of California, 94720, Berkeley, CA, USA",
"USDA/ARS Plant Gene Expression Center, 800 Buchanan St., 94710, Albany, CA, USA"
],
"type": "Organization"
},
"familyName": "Quail",
"givenName": "Peter H.",
"id": "sg:person.01355037356.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355037356.62"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nbt0990-833",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025361066",
"https://doi.org/10.1038/nbt0990-833"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00019304",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023000091",
"https://doi.org/10.1007/bf00019304"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00236093",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002037808",
"https://doi.org/10.1007/bf00236093"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00233416",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044719009",
"https://doi.org/10.1007/bf00233416"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00020010",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019204523",
"https://doi.org/10.1007/bf00020010"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00282445",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037797380",
"https://doi.org/10.1007/bf00282445"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00028868",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048061855",
"https://doi.org/10.1007/bf00028868"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00042079",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027369631",
"https://doi.org/10.1007/bf00042079"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02336778",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044808491",
"https://doi.org/10.1007/bf02336778"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0793-835",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041903612",
"https://doi.org/10.1038/nbt0793-835"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1293-1553",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017633667",
"https://doi.org/10.1038/nbt1293-1553"
],
"type": "CreativeWork"
}
],
"datePublished": "1996-05",
"datePublishedReg": "1996-05-01",
"description": "A set of plasmids has been constructed utilizing the promoter, 5\u2032 untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-\u03b2-glucuronidase (Ubi-GUS), and ubiquitin-phosphinothricin acetyl transferase (Ubi-bar) have been generated, as well as a construct containing chimaeric genes for bothUbi-GUS andUbi-bar in a single plasmid. Another construct was generated to allow cloning of protein coding sequences of choice onBam HI andBam HI-compatible restriction fragments downstream of theUbi-1 gene fragment. Because theUbi-1 promotor has been shown to be highly active in monocots, these constructs may be useful for generating high-level gene expression of selectable markers to facilitate efficient transformation of monocots, to drive expression of reference reporter genes in studies of gene expression, and to provide expression of biotechnologically important protein products in transgenic plants.",
"genre": "article",
"id": "sg:pub.10.1007/bf01969712",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1102526",
"issn": [
"0962-8819",
"1573-9368"
],
"name": "Transgenic Research",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "5"
}
],
"keywords": [
"chimaeric gene",
"gene expression",
"cloning of proteins",
"maize ubiquitin gene",
"high-level gene expression",
"screenable marker gene",
"set of plasmids",
"high-level expression",
"transgenic plants",
"monocotyledonous plants",
"expression of proteins",
"ubiquitin genes",
"selectable marker",
"untranslated exons",
"first intron",
"marker genes",
"reporter gene",
"gene fragments",
"protein products",
"acetyl transferase",
"restriction fragments",
"genes",
"monocots",
"single plasmid",
"plasmid",
"expression",
"plants",
"protein",
"efficient transformation",
"sequence",
"introns",
"fragments",
"cloning",
"exons",
"promoter",
"transferase",
"constructs",
"promotor",
"markers",
"sequence of choices",
"vector",
"products",
"transformation",
"study",
"set",
"choice",
"bar"
],
"name": "Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants",
"pagination": "213-218",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1002431148"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf01969712"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"8673150"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf01969712",
"https://app.dimensions.ai/details/publication/pub.1002431148"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:52",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_287.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/bf01969712"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01969712'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01969712'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01969712'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01969712'
This table displays all metadata directly associated to this object as RDF triples.
208 TRIPLES
21 PREDICATES
96 URIs
77 LITERALS
19 BLANK NODES