Expression of giant silkmoth cecropin B genes in tobacco View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-03

AUTHORS

Dion Florack, Sjefke Allefs, Rik Bollen, Dirk Bosch, Bert Visser, Willem Stiekema

ABSTRACT

Cecropin B is a small antibacterial peptide from the giant silkmoth Hyalophora cecropia. To reveal the potential of this peptide for engineering bacterial disease resistance into crops, several cecropin B gene constructs were made either for expression in the cytosol or for secretion. All constructs were cloned in a plant expression vector and introduced in tobacco via Agrobacterium tumefaciens. A cDNA-derived cecropin B gene construct lacking the amino-terminal signal peptide was poorly expressed in transgenic plants at the mRNA level, whereas plants harbouring a full-length cDNA-derived construct containing the insect signal peptide, showed increased cecropin B-mRNA levels. Highest expression was found in plants harbouring a construct with a plant-gene-derived signal peptide. In none of the transgenic plants could the cecropin B peptide be detected. This is most likely caused by breakdown of the peptide by plant endogenous proteases, since a chemically synthesized cecropin B peptide was degraded within seconds in various plant cell extracts. This degradation could be prevented by the addition of specific protease inhibitors and by boiling the extract prior to adding the peptide. In addition, anionic detergents, in contrast to cationic, zwitter-ionic or non-ionic detergents, could prevent this degradation. Nevertheless, transgenic tobacco plants were evaluated for resistance to Pseudomonas solanacearum, the causal agent of bacterial wilt of many crops, and P. syringae pv. tabaci, the causal agent of bacterial wildfire, which are highly susceptible to cecropin B in vitro. No resistance was found. These experiments indicate that introduction and expression of cecropin B genes in tobacco does not result in detectable cecropin B protein levels and resistance to bacterial infections, most likely due to degradation of the protein by endogenous proteases. More... »

PAGES

132-141

Journal

TITLE

Transgenic Research

ISSUE

2

VOLUME

4

Author Affiliations

Related Patents

  • Mammalian-Type Glycosylation In Transgenic Plants Expressing Mammalian Β1,4-Galactosyltransferase
  • Transgenic Plants Expressing Dermaseptin Peptides Providing Broad Spectrum Resistance To Pathogens
  • Gntiii Expression In Plants
  • Antimicrobial Peptides And Uses Thereof
  • Sp1 Polypeptides, Modified Sp1 Polypeptides And Uses Thereof
  • Transgenic Plants Expressing Galactosyltransferase And Sialyl Transferase
  • Method For Secretory Production Of Glycoprotein Having Human-Type Sugar Chain Using Plant Cell
  • Peptides With Enhanced Stability To Protease Degradation
  • Denaturant Stable And/Or Protease Resistant, Chaperone-Like Oligomeric Proteins, Polynucleotides Encoding Same, Their Uses And Methods Of Increasing A Specific Activity Thereof
  • Optimizing Glycan Processing In Plants
  • Gntiii Expression In Plants
  • Method Of Expressing Galactosyltransferase And Inhibiting Xylosyltransferase Or Fucosyltransferase In A Transgenic Plant Cell For Secretory Production Of Glycoproteins Having Human-Type Sugar Chains
  • Optimizing Glycan Processing In Plants
  • Plant Production Of Immunoglobulins With Reduced Fucosylation
  • Gntiii Expression In Plants
  • Mammalian-Type Glycosylation In Plants By Expression Of A Zebrafish Glycosyltransferase
  • Method For Manufacturing Glycoproteins Having Human-Type Glycosylation
  • Antimicrobial Peptides And Uses Thereof
  • Plant Expressing Mammalian Β1,4-Galactosyltransferase And Β1,3-Glucuronyltransferase
  • Transgenic Plants Expressing Temporin Peptides
  • Disease Resistance In Vitis
  • Mapping Molecular Interactions In Plants With Protein Fragments Complementation Assays
  • Method Of Co-Expressing Galactosyltransferase And A Glycoprotein In A Transgenic Plant Cell And Sialylating The Glycoprotein For Production Of Glycoprotein Having Human-Type Sugar Chain
  • Plant-Produced Glycoprotein Comprising Human-Type Sugar Chain
  • Mammalian-Type Glycosylation In Plants By Expression Of Non-Mammalian Glycosyltransferases
  • Mammalian-Type Glycosylation In Plants
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01969415

    DOI

    http://dx.doi.org/10.1007/bf01969415

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014666035

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7704053


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bombyx", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Complementary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Insect Hormones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Insect Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Toxic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tobacco", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Florack", 
            "givenName": "Dion", 
            "id": "sg:person.01361704367.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361704367.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Allefs", 
            "givenName": "Sjefke", 
            "id": "sg:person.01134111075.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134111075.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bollen", 
            "givenName": "Rik", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bosch", 
            "givenName": "Dirk", 
            "id": "sg:person.0605230770.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605230770.21"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Visser", 
            "givenName": "Bert", 
            "id": "sg:person.01256112050.74", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256112050.74"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stiekema", 
            "givenName": "Willem", 
            "id": "sg:person.01065316404.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065316404.19"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1073/pnas.85.14.5072", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001880258"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(87)80540-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002934716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0168-9452(92)90012-b", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004778818"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(82)80368-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005877393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.7.8.2914", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011244096"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.8.2240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012423410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/252169a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013100174", 
              "https://doi.org/10.1038/252169a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0168-9452(93)90169-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013697683"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013909718", 
              "https://doi.org/10.1007/bf00331014"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.mi.41.100187.000535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019331093"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.2.1.51", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019818596"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.74.12.5463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025360556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00016075", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025381986", 
              "https://doi.org/10.1007/bf00016075"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00016075", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025381986", 
              "https://doi.org/10.1007/bf00016075"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025529346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00233324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026648871", 
              "https://doi.org/10.1007/bf00233324"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00233324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026648871", 
              "https://doi.org/10.1007/bf00233324"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.2.488", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026991187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bies.950060605", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029323683"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/229174a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030055541", 
              "https://doi.org/10.1038/229174a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040576", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032753810", 
              "https://doi.org/10.1007/bf00040576"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040576", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032753810", 
              "https://doi.org/10.1007/bf00040576"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-1759(90)90428-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038141525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-1759(90)90428-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038141525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0048-4059(82)90002-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039645721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/199299b0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045783220", 
              "https://doi.org/10.1038/199299b0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.8.3324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046309389"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1982.tb06857.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050031523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00420a008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055175107"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi-5-322", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060079260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/pd-70-887", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060083876"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/phyto-67-1051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060104977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.236.4806.1299", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062534239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/3869050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1070467134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079456809", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079687101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1987.tb04716.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079819476"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1995-03", 
        "datePublishedReg": "1995-03-01", 
        "description": "Cecropin B is a small antibacterial peptide from the giant silkmoth Hyalophora cecropia. To reveal the potential of this peptide for engineering bacterial disease resistance into crops, several cecropin B gene constructs were made either for expression in the cytosol or for secretion. All constructs were cloned in a plant expression vector and introduced in tobacco via Agrobacterium tumefaciens. A cDNA-derived cecropin B gene construct lacking the amino-terminal signal peptide was poorly expressed in transgenic plants at the mRNA level, whereas plants harbouring a full-length cDNA-derived construct containing the insect signal peptide, showed increased cecropin B-mRNA levels. Highest expression was found in plants harbouring a construct with a plant-gene-derived signal peptide. In none of the transgenic plants could the cecropin B peptide be detected. This is most likely caused by breakdown of the peptide by plant endogenous proteases, since a chemically synthesized cecropin B peptide was degraded within seconds in various plant cell extracts. This degradation could be prevented by the addition of specific protease inhibitors and by boiling the extract prior to adding the peptide. In addition, anionic detergents, in contrast to cationic, zwitter-ionic or non-ionic detergents, could prevent this degradation. Nevertheless, transgenic tobacco plants were evaluated for resistance to Pseudomonas solanacearum, the causal agent of bacterial wilt of many crops, and P. syringae pv. tabaci, the causal agent of bacterial wildfire, which are highly susceptible to cecropin B in vitro. No resistance was found. These experiments indicate that introduction and expression of cecropin B genes in tobacco does not result in detectable cecropin B protein levels and resistance to bacterial infections, most likely due to degradation of the protein by endogenous proteases.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf01969415", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1102526", 
            "issn": [
              "0962-8819", 
              "1573-9368"
            ], 
            "name": "Transgenic Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "4"
          }
        ], 
        "name": "Expression of giant silkmoth cecropin B genes in tobacco", 
        "pagination": "132-141", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "45df14bed1e5af2abc886779266575674e1d90a627b54841c794810b3f1b1c1c"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "7704053"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9209120"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf01969415"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1014666035"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf01969415", 
          "https://app.dimensions.ai/details/publication/pub.1014666035"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T11:23", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000355_0000000355/records_52987_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF01969415"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01969415'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01969415'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01969415'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01969415'


     

    This table displays all metadata directly associated to this object as RDF triples.

    276 TRIPLES      21 PREDICATES      79 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf01969415 schema:about N2af5482a2ab64ee4933bb282e86024b2
    2 N5c4c510ba12e434684cf0d5ef594e05c
    3 N6a3504e6a7914749a9e5f922e067497e
    4 N6ffe4274088e4802993715ef0aec0d42
    5 N711e4ba52f8f4574917de90c8b1a6d85
    6 N749ebccd16d941f19192aa37e62b91d4
    7 N9851afccc1204fd3a8e313473506c378
    8 Na88a39588d934cd6ad2f4ee70281a583
    9 Nc225ffcee6fd4da8a5d84b731910b4d6
    10 Nc2d0ffc1ca1c4eb9b1b2375260aae427
    11 Ncbcd8128ee5141ea89521158589bf25c
    12 Ncf18388c2def4fa8927d9f2904638a0d
    13 Nd9dcc7cf73014a7d9ebf806330a897bc
    14 Ne25e37915f974302bcbd5b45d58232ff
    15 Ne373b21df7084e928b8e0ca55b3ffe2e
    16 Nf943ce21a3d741cd86ea865e5daf1380
    17 Nfde57c7b6fe747b99813195ea35b62c0
    18 anzsrc-for:06
    19 anzsrc-for:0607
    20 schema:author N4ede7bad6e374a81844d4e0989ae5eb0
    21 schema:citation sg:pub.10.1007/bf00016075
    22 sg:pub.10.1007/bf00040576
    23 sg:pub.10.1007/bf00233324
    24 sg:pub.10.1007/bf00331014
    25 sg:pub.10.1038/199299b0
    26 sg:pub.10.1038/229174a0
    27 sg:pub.10.1038/252169a0
    28 https://app.dimensions.ai/details/publication/pub.1079456809
    29 https://doi.org/10.1002/bies.950060605
    30 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
    31 https://doi.org/10.1002/j.1460-2075.1987.tb04716.x
    32 https://doi.org/10.1016/0003-2697(76)90527-3
    33 https://doi.org/10.1016/0014-5793(82)80368-2
    34 https://doi.org/10.1016/0014-5793(87)80540-9
    35 https://doi.org/10.1016/0022-1759(90)90428-x
    36 https://doi.org/10.1016/0048-4059(82)90002-9
    37 https://doi.org/10.1016/0168-9452(92)90012-b
    38 https://doi.org/10.1016/0168-9452(93)90169-z
    39 https://doi.org/10.1021/bi00420a008
    40 https://doi.org/10.1073/pnas.74.12.5463
    41 https://doi.org/10.1073/pnas.82.2.488
    42 https://doi.org/10.1073/pnas.82.8.2240
    43 https://doi.org/10.1073/pnas.85.14.5072
    44 https://doi.org/10.1073/pnas.88.8.3324
    45 https://doi.org/10.1094/mpmi-5-322
    46 https://doi.org/10.1094/pd-70-887
    47 https://doi.org/10.1094/phyto-67-1051
    48 https://doi.org/10.1105/tpc.2.1.51
    49 https://doi.org/10.1111/j.1432-1033.1982.tb06857.x
    50 https://doi.org/10.1126/science.236.4806.1299
    51 https://doi.org/10.1128/mcb.7.8.2914
    52 https://doi.org/10.1146/annurev.mi.41.100187.000535
    53 https://doi.org/10.2307/3869050
    54 schema:datePublished 1995-03
    55 schema:datePublishedReg 1995-03-01
    56 schema:description Cecropin B is a small antibacterial peptide from the giant silkmoth Hyalophora cecropia. To reveal the potential of this peptide for engineering bacterial disease resistance into crops, several cecropin B gene constructs were made either for expression in the cytosol or for secretion. All constructs were cloned in a plant expression vector and introduced in tobacco via Agrobacterium tumefaciens. A cDNA-derived cecropin B gene construct lacking the amino-terminal signal peptide was poorly expressed in transgenic plants at the mRNA level, whereas plants harbouring a full-length cDNA-derived construct containing the insect signal peptide, showed increased cecropin B-mRNA levels. Highest expression was found in plants harbouring a construct with a plant-gene-derived signal peptide. In none of the transgenic plants could the cecropin B peptide be detected. This is most likely caused by breakdown of the peptide by plant endogenous proteases, since a chemically synthesized cecropin B peptide was degraded within seconds in various plant cell extracts. This degradation could be prevented by the addition of specific protease inhibitors and by boiling the extract prior to adding the peptide. In addition, anionic detergents, in contrast to cationic, zwitter-ionic or non-ionic detergents, could prevent this degradation. Nevertheless, transgenic tobacco plants were evaluated for resistance to Pseudomonas solanacearum, the causal agent of bacterial wilt of many crops, and P. syringae pv. tabaci, the causal agent of bacterial wildfire, which are highly susceptible to cecropin B in vitro. No resistance was found. These experiments indicate that introduction and expression of cecropin B genes in tobacco does not result in detectable cecropin B protein levels and resistance to bacterial infections, most likely due to degradation of the protein by endogenous proteases.
    57 schema:genre research_article
    58 schema:inLanguage en
    59 schema:isAccessibleForFree false
    60 schema:isPartOf N0cf062f54ff44e61987efd80a1206cb4
    61 N442c73d7ec584e51aafc010917387153
    62 sg:journal.1102526
    63 schema:name Expression of giant silkmoth cecropin B genes in tobacco
    64 schema:pagination 132-141
    65 schema:productId N28b8c96230094da584de2566f21b9a62
    66 N7212d9a177994efdb6f8bc2145abe77a
    67 N7f4fcd425ff54d37af275f3df923ac02
    68 Nb825585390614d02b81dae3aea64a1a0
    69 Nf09e74cace3240d2a111594746aa6dd4
    70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014666035
    71 https://doi.org/10.1007/bf01969415
    72 schema:sdDatePublished 2019-04-11T11:23
    73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    74 schema:sdPublisher N08f4a60de3804fedafcad9c80f71a412
    75 schema:url http://link.springer.com/10.1007/BF01969415
    76 sgo:license sg:explorer/license/
    77 sgo:sdDataset articles
    78 rdf:type schema:ScholarlyArticle
    79 N05ec5ae12c0f489cb1ba1c32a4d6dbac schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    80 schema:familyName Bollen
    81 schema:givenName Rik
    82 rdf:type schema:Person
    83 N08f4a60de3804fedafcad9c80f71a412 schema:name Springer Nature - SN SciGraph project
    84 rdf:type schema:Organization
    85 N0cf062f54ff44e61987efd80a1206cb4 schema:issueNumber 2
    86 rdf:type schema:PublicationIssue
    87 N1f1a027274e74acfa481e1518fca4478 rdf:first sg:person.01065316404.19
    88 rdf:rest rdf:nil
    89 N28b8c96230094da584de2566f21b9a62 schema:name pubmed_id
    90 schema:value 7704053
    91 rdf:type schema:PropertyValue
    92 N2af5482a2ab64ee4933bb282e86024b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Insect Hormones
    94 rdf:type schema:DefinedTerm
    95 N442c73d7ec584e51aafc010917387153 schema:volumeNumber 4
    96 rdf:type schema:PublicationVolume
    97 N4ede7bad6e374a81844d4e0989ae5eb0 rdf:first sg:person.01361704367.20
    98 rdf:rest N8a2db845af7540268af175759dd85302
    99 N5ae8ce82a3b7451f907cbde8fb8d7177 rdf:first sg:person.0605230770.21
    100 rdf:rest N91f8819b52724392a19f7fc087a93a65
    101 N5c4c510ba12e434684cf0d5ef594e05c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Plants, Toxic
    103 rdf:type schema:DefinedTerm
    104 N5ca38d1f6c99458ab3efe30a56a0c698 rdf:first N05ec5ae12c0f489cb1ba1c32a4d6dbac
    105 rdf:rest N5ae8ce82a3b7451f907cbde8fb8d7177
    106 N6a3504e6a7914749a9e5f922e067497e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name DNA Primers
    108 rdf:type schema:DefinedTerm
    109 N6ffe4274088e4802993715ef0aec0d42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Gene Expression
    111 rdf:type schema:DefinedTerm
    112 N711e4ba52f8f4574917de90c8b1a6d85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Insect Proteins
    114 rdf:type schema:DefinedTerm
    115 N7212d9a177994efdb6f8bc2145abe77a schema:name readcube_id
    116 schema:value 45df14bed1e5af2abc886779266575674e1d90a627b54841c794810b3f1b1c1c
    117 rdf:type schema:PropertyValue
    118 N749ebccd16d941f19192aa37e62b91d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Bombyx
    120 rdf:type schema:DefinedTerm
    121 N7f4fcd425ff54d37af275f3df923ac02 schema:name doi
    122 schema:value 10.1007/bf01969415
    123 rdf:type schema:PropertyValue
    124 N8a2db845af7540268af175759dd85302 rdf:first sg:person.01134111075.28
    125 rdf:rest N5ca38d1f6c99458ab3efe30a56a0c698
    126 N91f8819b52724392a19f7fc087a93a65 rdf:first sg:person.01256112050.74
    127 rdf:rest N1f1a027274e74acfa481e1518fca4478
    128 N9851afccc1204fd3a8e313473506c378 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Genetic Vectors
    130 rdf:type schema:DefinedTerm
    131 Na88a39588d934cd6ad2f4ee70281a583 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Molecular Sequence Data
    133 rdf:type schema:DefinedTerm
    134 Nb825585390614d02b81dae3aea64a1a0 schema:name nlm_unique_id
    135 schema:value 9209120
    136 rdf:type schema:PropertyValue
    137 Nc225ffcee6fd4da8a5d84b731910b4d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name DNA, Complementary
    139 rdf:type schema:DefinedTerm
    140 Nc2d0ffc1ca1c4eb9b1b2375260aae427 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Tobacco
    142 rdf:type schema:DefinedTerm
    143 Ncbcd8128ee5141ea89521158589bf25c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Animals
    145 rdf:type schema:DefinedTerm
    146 Ncf18388c2def4fa8927d9f2904638a0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Protein Engineering
    148 rdf:type schema:DefinedTerm
    149 Nd9dcc7cf73014a7d9ebf806330a897bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Polymerase Chain Reaction
    151 rdf:type schema:DefinedTerm
    152 Ne25e37915f974302bcbd5b45d58232ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Plants, Genetically Modified
    154 rdf:type schema:DefinedTerm
    155 Ne373b21df7084e928b8e0ca55b3ffe2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Amino Acid Sequence
    157 rdf:type schema:DefinedTerm
    158 Nf09e74cace3240d2a111594746aa6dd4 schema:name dimensions_id
    159 schema:value pub.1014666035
    160 rdf:type schema:PropertyValue
    161 Nf943ce21a3d741cd86ea865e5daf1380 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    162 schema:name Transformation, Genetic
    163 rdf:type schema:DefinedTerm
    164 Nfde57c7b6fe747b99813195ea35b62c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    165 schema:name Base Sequence
    166 rdf:type schema:DefinedTerm
    167 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    168 schema:name Biological Sciences
    169 rdf:type schema:DefinedTerm
    170 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    171 schema:name Plant Biology
    172 rdf:type schema:DefinedTerm
    173 sg:journal.1102526 schema:issn 0962-8819
    174 1573-9368
    175 schema:name Transgenic Research
    176 rdf:type schema:Periodical
    177 sg:person.01065316404.19 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    178 schema:familyName Stiekema
    179 schema:givenName Willem
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065316404.19
    181 rdf:type schema:Person
    182 sg:person.01134111075.28 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    183 schema:familyName Allefs
    184 schema:givenName Sjefke
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134111075.28
    186 rdf:type schema:Person
    187 sg:person.01256112050.74 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    188 schema:familyName Visser
    189 schema:givenName Bert
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256112050.74
    191 rdf:type schema:Person
    192 sg:person.01361704367.20 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    193 schema:familyName Florack
    194 schema:givenName Dion
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361704367.20
    196 rdf:type schema:Person
    197 sg:person.0605230770.21 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    198 schema:familyName Bosch
    199 schema:givenName Dirk
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605230770.21
    201 rdf:type schema:Person
    202 sg:pub.10.1007/bf00016075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025381986
    203 https://doi.org/10.1007/bf00016075
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1007/bf00040576 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032753810
    206 https://doi.org/10.1007/bf00040576
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1007/bf00233324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026648871
    209 https://doi.org/10.1007/bf00233324
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1007/bf00331014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013909718
    212 https://doi.org/10.1007/bf00331014
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1038/199299b0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045783220
    215 https://doi.org/10.1038/199299b0
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/229174a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030055541
    218 https://doi.org/10.1038/229174a0
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/252169a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013100174
    221 https://doi.org/10.1038/252169a0
    222 rdf:type schema:CreativeWork
    223 https://app.dimensions.ai/details/publication/pub.1079456809 schema:CreativeWork
    224 https://doi.org/10.1002/bies.950060605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029323683
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079687101
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1002/j.1460-2075.1987.tb04716.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079819476
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1016/0014-5793(82)80368-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005877393
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/0014-5793(87)80540-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002934716
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/0022-1759(90)90428-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038141525
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1016/0048-4059(82)90002-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039645721
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1016/0168-9452(92)90012-b schema:sameAs https://app.dimensions.ai/details/publication/pub.1004778818
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/0168-9452(93)90169-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1013697683
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1021/bi00420a008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055175107
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1073/pnas.74.12.5463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025360556
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1073/pnas.82.8.2240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012423410
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1073/pnas.85.14.5072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001880258
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1073/pnas.88.8.3324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046309389
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1094/mpmi-5-322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060079260
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1094/pd-70-887 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060083876
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1094/phyto-67-1051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060104977
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1105/tpc.2.1.51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019818596
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1111/j.1432-1033.1982.tb06857.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050031523
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1126/science.236.4806.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062534239
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1128/mcb.7.8.2914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011244096
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1146/annurev.mi.41.100187.000535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019331093
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.2307/3869050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467134
    273 rdf:type schema:CreativeWork
    274 https://www.grid.ac/institutes/grid.4818.5 schema:alternateName Wageningen University & Research
    275 schema:name Department of Molecular Biology, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands
    276 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...