Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-10

AUTHORS

E. H. H. Wiltink, P. Stekkinger, J. A. C. Brakenhoff, S. A. Danner

ABSTRACT

In this article a simple method for the determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids is presented, based on reversed-phase ion-pair HPLC with UV detection at 254 nm. No complicated extraction procedure is needed. The stationary phase consists of a reversed-phase C18 stainless steel column and the mobile phase of 0.005M sodium acetate and 0.0025M pentanesulfonic acid sodium salt (PIC-B5) as an ion-pair reagent in water (pH 6.5). DHPG is a new antiviral drug in research of the treatment of cytomegalovirus infections. As a pilot study serum and urine samples of a few patients were investigated to gain an impression of the therapeutic range of DHPG. The method is suitable for routine assay of DHPG. More... »

PAGES

261-264

References to SciGraph publications

  • 1983-11. Acyclovir in DRUGS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01953628

    DOI

    http://dx.doi.org/10.1007/bf01953628

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010075640

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3501569


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