Single DNA marker generated by “YAC-Alu PCR” that is end-specific View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1991-09

AUTHORS

Hiroyuki Tashiro, Kazuo Ozawa, Xiaoren Tang, Hiroshi Nakai, Toshihiko Eki, Yasufumi Murakami, Ei-ichi Soeda, Kazushige Yokoyama

ABSTRACT

A simple strategy for the rapid preparation of an end-specific linking-DNA probe from the YAC-human chromosome 21 DNA recombinant clone and the characterization of this single DNA probe are described. Synthetic oligodeoxynucleotide primers, based on the consensus Alu sequence, and the Sup4 DNA fragment in the YAC arms were used to amplify end-specific DNA sequences by the polymerase chain reaction (PCR) for screening of the linking YAC recombinant clones ("YAC-Alu PCR"). Nucleotide sequencing of the product of PCR from human genomic DNA in a YAC insert confirmed the boundary between the vector and the insert and the presence of the 3'-end Alu-like structure. The probe R1, prepared by "YAC-Alu PCR" amplification, was assigned to chromosome 21 by Southern hybridization of somatic cell hybrid DNAs. In situ hybridization allowed localization of the R1 DNA probe to the human chromosome 21q21-q22.1 region. Thus, this approach has significant advantages not only for isolation of a single DNA probe specific for human chromosome 21 but also for the screening of YAC linking recombinant clones for mapping of the human genome. More... »

PAGES

229-243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01910541

DOI

http://dx.doi.org/10.1007/bf01910541

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042831904

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1753436


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01910541'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01910541'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01910541'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01910541'


 

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