A simple cryopreservation method of human blood for chromosome study View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1981-09

AUTHORS

Koji Kondo, Motomichi Sasaki

ABSTRACT

A method is described of cryopreservation of human lymphocytes for cytogenetic studies. An aliquot of whole blood is pre-incubated in a PHA-added medium for at least one hour at 37°C, and then stored frozen at −140°C in Eagle's MEM containing 10% fetal bovine serum and 10% DMSO for various periods up to one year. Thawing is made quickly in a water-bath at 37°C. After thorough washing with MEM, the routine PHA-added blood culture method is applied, and airdried chromosome preparations are made. The results are highly reproducible with a good number of mitotic cells sufficient for karyotyping. More... »

PAGES

255-259

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01896138

DOI

http://dx.doi.org/10.1007/bf01896138

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022474468

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7334694


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