Splase: A new class IIs zinc-finger restriction endonuclease with specificity for Sp1 binding sites View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-07

AUTHORS

Baohua Huang, Christopher J. Schaeffer, Qunhui Li, Ming-Daw Tsai

ABSTRACT

A new restriction endonuclease, named Splase, was constructed by genetically fusing the DNA-cleavage domain of the restriction endonuclease Fok1 with the zinc-finger DNA-binding domain of the transcription factor Sp1. The resulting protein was expressed in Escherichia coli., partially purified, and shown to selectively digest plasmid DNA harboring consensus Sp1 sites. Splase was also shown to selectively digest the long terminal repeat of the HIV-1 DNA at Sp1 sites. Splase recognizes a 10-bp DNA sequence and hydrolyzes phosphodiester bonds upstream of the binding sequence. The binding specificity of Splase makes this a "rare cutter" restriction enzyme which could be valuable in creating large DNA fragments for genome sequencing projects. The result also presents the opportunity to create other restriction enzymes by altering the binding specificity of the zinc-finger recognition helix. More... »

PAGES

481-489

Journal

TITLE

The Protein Journal

ISSUE

5

VOLUME

15

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01886856

DOI

http://dx.doi.org/10.1007/bf01886856

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036882003

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8895094


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Deoxyribonucleases, Type II Site-Specific", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophoresis, Polyacrylamide Gel", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Restriction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sp1 Transcription Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zinc Fingers", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Ohio State Biochemistry Program, Ohio State University, 43210, Columbus, Ohio"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Baohua", 
        "id": "sg:person.01243266440.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243266440.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Ohio State Biochemistry Program, Ohio State University, 43210, Columbus, Ohio"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schaeffer", 
        "givenName": "Christopher J.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Department of Chemistry, Ohio State University, 43210, Columbus, Ohio"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Qunhui", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Ohio State Biochemistry Program, Ohio State University, 43210, Columbus, Ohio", 
            "Department of Chemistry, Ohio State University, 43210, Columbus, Ohio", 
            "Department of Biochemistry, Ohio State University, 43210, Columbus, Ohio"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsai", 
        "givenName": "Ming-Daw", 
        "id": "sg:person.01367011750.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367011750.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.93.3.1156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001210891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.20.9759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001737792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90229-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002634445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90229-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002634445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.7.2764", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003396364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(80)65059-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008420927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1994.tb01022.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010142673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(81)90062-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012360299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(81)90062-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012360299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.24.9759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012682694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90594-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012968110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0021-9673(00)93969-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021046676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.9.4206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033727067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.23.11163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034032969"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.10.4275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034119268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.77.7.4170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036672028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.3.883", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039714161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90738-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041873164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90738-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041873164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4781(94)90061-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047501830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4781(94)90061-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047501830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00067a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00447a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055176582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00167a085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055710957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00204a038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055714094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00227a052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055715906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2028256", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062518816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2479980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062539407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2820056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062563612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3008338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062582068"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-07", 
    "datePublishedReg": "1996-07-01", 
    "description": "A new restriction endonuclease, named Splase, was constructed by genetically fusing the DNA-cleavage domain of the restriction endonuclease Fok1 with the zinc-finger DNA-binding domain of the transcription factor Sp1. The resulting protein was expressed in Escherichia coli., partially purified, and shown to selectively digest plasmid DNA harboring consensus Sp1 sites. Splase was also shown to selectively digest the long terminal repeat of the HIV-1 DNA at Sp1 sites. Splase recognizes a 10-bp DNA sequence and hydrolyzes phosphodiester bonds upstream of the binding sequence. The binding specificity of Splase makes this a \"rare cutter\" restriction enzyme which could be valuable in creating large DNA fragments for genome sequencing projects. The result also presents the opportunity to create other restriction enzymes by altering the binding specificity of the zinc-finger recognition helix.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf01886856", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2513091", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2509946", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2512816", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1297375", 
        "issn": [
          "1572-3887", 
          "1573-4943"
        ], 
        "name": "The Protein Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Splase: A new class IIs zinc-finger restriction endonuclease with specificity for Sp1 binding sites", 
    "pagination": "481-489", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "afb10f372375541556e1d6c9d7bd177c9f01aeeb65af1285f18da72c7d024bb4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8895094"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8217321"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01886856"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036882003"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01886856", 
      "https://app.dimensions.ai/details/publication/pub.1036882003"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000351_0000000351/records_43266_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF01886856"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01886856'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01886856'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01886856'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01886856'


 

This table displays all metadata directly associated to this object as RDF triples.

226 TRIPLES      21 PREDICATES      68 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01886856 schema:about N0d40323b33a949a7adbec681c47b0dae
2 N1677294fa89f4bf790e0d053884bd226
3 N1dd4d19714bb45ae90571c526b6d8c85
4 N2ece593b31a14de2af733f9a84c3235a
5 N38b270279a024262abcb8a25a4a022a4
6 N3edc84d364334cb49ab5fd2cdb1ef5cd
7 N3ef000e2a79041b0b71dd0ab991d5e76
8 N700a6073d4714ebb8127c4ced076861b
9 Na4d4b2c95c1142b99ce86aa5a1cecfb9
10 Nad2cc47c61de41aaa93fed67820002f6
11 Nc73a174350264a88906ffac93ff4c70d
12 Nf02b6eb8a4514e71883ed25936719aca
13 Nf08661d455824687b2d04b3380196e48
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N3cc0e1355e0d483ebf9bd71f3228cebd
17 schema:citation https://doi.org/10.1016/0092-8674(87)90594-0
18 https://doi.org/10.1016/0167-4781(94)90061-2
19 https://doi.org/10.1016/0378-1119(81)90062-7
20 https://doi.org/10.1016/0378-1119(90)90229-k
21 https://doi.org/10.1016/0378-1119(93)90738-o
22 https://doi.org/10.1016/s0021-9673(00)93969-4
23 https://doi.org/10.1016/s0076-6879(80)65059-9
24 https://doi.org/10.1021/bi00067a009
25 https://doi.org/10.1021/bi00447a004
26 https://doi.org/10.1021/ja00167a085
27 https://doi.org/10.1021/ja00204a038
28 https://doi.org/10.1021/ja00227a052
29 https://doi.org/10.1073/pnas.77.7.4170
30 https://doi.org/10.1073/pnas.83.24.9759
31 https://doi.org/10.1073/pnas.89.10.4275
32 https://doi.org/10.1073/pnas.89.20.9759
33 https://doi.org/10.1073/pnas.90.7.2764
34 https://doi.org/10.1073/pnas.90.9.4206
35 https://doi.org/10.1073/pnas.91.23.11163
36 https://doi.org/10.1073/pnas.91.3.883
37 https://doi.org/10.1073/pnas.93.3.1156
38 https://doi.org/10.1111/j.1365-2958.1994.tb01022.x
39 https://doi.org/10.1126/science.2028256
40 https://doi.org/10.1126/science.2479980
41 https://doi.org/10.1126/science.2820056
42 https://doi.org/10.1126/science.3008338
43 schema:datePublished 1996-07
44 schema:datePublishedReg 1996-07-01
45 schema:description A new restriction endonuclease, named Splase, was constructed by genetically fusing the DNA-cleavage domain of the restriction endonuclease Fok1 with the zinc-finger DNA-binding domain of the transcription factor Sp1. The resulting protein was expressed in Escherichia coli., partially purified, and shown to selectively digest plasmid DNA harboring consensus Sp1 sites. Splase was also shown to selectively digest the long terminal repeat of the HIV-1 DNA at Sp1 sites. Splase recognizes a 10-bp DNA sequence and hydrolyzes phosphodiester bonds upstream of the binding sequence. The binding specificity of Splase makes this a "rare cutter" restriction enzyme which could be valuable in creating large DNA fragments for genome sequencing projects. The result also presents the opportunity to create other restriction enzymes by altering the binding specificity of the zinc-finger recognition helix.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree false
49 schema:isPartOf Na4e3b11ba5ce4fc086c9cffdff9d95e8
50 Naa639f46355b4abcbc6e127851454b63
51 sg:journal.1297375
52 schema:name Splase: A new class IIs zinc-finger restriction endonuclease with specificity for Sp1 binding sites
53 schema:pagination 481-489
54 schema:productId N1d687e8d88c24030826d073813a9220e
55 N2953f709250748789026f28a6482bfe0
56 N2c5295ae13604559b1d5d09519bcd380
57 N2d6b9f1948f34fe3a58ba30c2d266d26
58 N659fb1e2ea3f4adcaac2ba974811269c
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036882003
60 https://doi.org/10.1007/bf01886856
61 schema:sdDatePublished 2019-04-11T10:55
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher Nd56f2bec4d7f43f39d1fdcc56e7e2b8d
64 schema:url http://link.springer.com/10.1007/BF01886856
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N0d40323b33a949a7adbec681c47b0dae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Sp1 Transcription Factor
70 rdf:type schema:DefinedTerm
71 N1677294fa89f4bf790e0d053884bd226 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Transfection
73 rdf:type schema:DefinedTerm
74 N1d687e8d88c24030826d073813a9220e schema:name nlm_unique_id
75 schema:value 8217321
76 rdf:type schema:PropertyValue
77 N1dd4d19714bb45ae90571c526b6d8c85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Binding Sites
79 rdf:type schema:DefinedTerm
80 N2953f709250748789026f28a6482bfe0 schema:name dimensions_id
81 schema:value pub.1036882003
82 rdf:type schema:PropertyValue
83 N2c5295ae13604559b1d5d09519bcd380 schema:name readcube_id
84 schema:value afb10f372375541556e1d6c9d7bd177c9f01aeeb65af1285f18da72c7d024bb4
85 rdf:type schema:PropertyValue
86 N2d6b9f1948f34fe3a58ba30c2d266d26 schema:name doi
87 schema:value 10.1007/bf01886856
88 rdf:type schema:PropertyValue
89 N2ece593b31a14de2af733f9a84c3235a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Escherichia coli
91 rdf:type schema:DefinedTerm
92 N38b270279a024262abcb8a25a4a022a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Base Sequence
94 rdf:type schema:DefinedTerm
95 N3cc0e1355e0d483ebf9bd71f3228cebd rdf:first sg:person.01243266440.58
96 rdf:rest Ncc76ac85000047f0aeb8c75dcc96a97f
97 N3edc84d364334cb49ab5fd2cdb1ef5cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Recombinant Fusion Proteins
99 rdf:type schema:DefinedTerm
100 N3ef000e2a79041b0b71dd0ab991d5e76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Restriction Mapping
102 rdf:type schema:DefinedTerm
103 N44ebc6eb953e49fc8695a1687d434c2c schema:affiliation https://www.grid.ac/institutes/grid.261331.4
104 schema:familyName Schaeffer
105 schema:givenName Christopher J.
106 rdf:type schema:Person
107 N659fb1e2ea3f4adcaac2ba974811269c schema:name pubmed_id
108 schema:value 8895094
109 rdf:type schema:PropertyValue
110 N700a6073d4714ebb8127c4ced076861b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Deoxyribonucleases, Type II Site-Specific
112 rdf:type schema:DefinedTerm
113 N90d93412ce0f49f38dde0677d4a7f030 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
114 schema:familyName Li
115 schema:givenName Qunhui
116 rdf:type schema:Person
117 Na4d4b2c95c1142b99ce86aa5a1cecfb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Plasmids
119 rdf:type schema:DefinedTerm
120 Na4e3b11ba5ce4fc086c9cffdff9d95e8 schema:volumeNumber 15
121 rdf:type schema:PublicationVolume
122 Naa639f46355b4abcbc6e127851454b63 schema:issueNumber 5
123 rdf:type schema:PublicationIssue
124 Nad2cc47c61de41aaa93fed67820002f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Electrophoresis, Polyacrylamide Gel
126 rdf:type schema:DefinedTerm
127 Nc5855a3e687d4fc09bebaf5d9bbc5930 rdf:first sg:person.01367011750.02
128 rdf:rest rdf:nil
129 Nc73a174350264a88906ffac93ff4c70d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Molecular Sequence Data
131 rdf:type schema:DefinedTerm
132 Ncc76ac85000047f0aeb8c75dcc96a97f rdf:first N44ebc6eb953e49fc8695a1687d434c2c
133 rdf:rest Ne7a67ad433b045ab904b4e259b04647c
134 Nd56f2bec4d7f43f39d1fdcc56e7e2b8d schema:name Springer Nature - SN SciGraph project
135 rdf:type schema:Organization
136 Ne7a67ad433b045ab904b4e259b04647c rdf:first N90d93412ce0f49f38dde0677d4a7f030
137 rdf:rest Nc5855a3e687d4fc09bebaf5d9bbc5930
138 Nf02b6eb8a4514e71883ed25936719aca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name DNA
140 rdf:type schema:DefinedTerm
141 Nf08661d455824687b2d04b3380196e48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Zinc Fingers
143 rdf:type schema:DefinedTerm
144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
145 schema:name Biological Sciences
146 rdf:type schema:DefinedTerm
147 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
148 schema:name Genetics
149 rdf:type schema:DefinedTerm
150 sg:grant.2509946 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01886856
151 rdf:type schema:MonetaryGrant
152 sg:grant.2512816 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01886856
153 rdf:type schema:MonetaryGrant
154 sg:grant.2513091 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01886856
155 rdf:type schema:MonetaryGrant
156 sg:journal.1297375 schema:issn 1572-3887
157 1573-4943
158 schema:name The Protein Journal
159 rdf:type schema:Periodical
160 sg:person.01243266440.58 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
161 schema:familyName Huang
162 schema:givenName Baohua
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243266440.58
164 rdf:type schema:Person
165 sg:person.01367011750.02 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
166 schema:familyName Tsai
167 schema:givenName Ming-Daw
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367011750.02
169 rdf:type schema:Person
170 https://doi.org/10.1016/0092-8674(87)90594-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012968110
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/0167-4781(94)90061-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047501830
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/0378-1119(81)90062-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012360299
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/0378-1119(90)90229-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1002634445
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/0378-1119(93)90738-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1041873164
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/s0021-9673(00)93969-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021046676
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0076-6879(80)65059-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008420927
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1021/bi00067a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159524
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1021/bi00447a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055176582
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1021/ja00167a085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055710957
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1021/ja00204a038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055714094
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1021/ja00227a052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055715906
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1073/pnas.77.7.4170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036672028
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1073/pnas.83.24.9759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012682694
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1073/pnas.89.10.4275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034119268
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1073/pnas.89.20.9759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001737792
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1073/pnas.90.7.2764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003396364
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1073/pnas.90.9.4206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033727067
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1073/pnas.91.23.11163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034032969
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1073/pnas.91.3.883 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039714161
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1073/pnas.93.3.1156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001210891
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1111/j.1365-2958.1994.tb01022.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010142673
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1126/science.2028256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062518816
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1126/science.2479980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062539407
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1126/science.2820056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062563612
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1126/science.3008338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062582068
221 rdf:type schema:CreativeWork
222 https://www.grid.ac/institutes/grid.261331.4 schema:alternateName The Ohio State University
223 schema:name Department of Biochemistry, Ohio State University, 43210, Columbus, Ohio
224 Department of Chemistry, Ohio State University, 43210, Columbus, Ohio
225 Ohio State Biochemistry Program, Ohio State University, 43210, Columbus, Ohio
226 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...