Chemical modification of notexin fromNotechis scutatus scutatus (Australian tiger snake) venom with pyridoxal-5′-phosphate View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-07

AUTHORS

Long-Sen Chang

ABSTRACT

Notexin fromNotechis scutatus scutatus snake venom was subjected to Lys modification with pyridoxal 5′-phosphate (PLP), and one major modified derivative was purified on a cation-exchanger SP-8HR column. The results of amino acid analysis and sequence determination revealed that only 2 Lys residues at positions 82 and 115 out of 11 Lys residues in notexin were modified. The incorporation of PLP into the protein was accompanied by the loss of 53% lethal toxicity, but the modified notexin showed an about 1.2-fold increase in enzymatic activity. However, the secondary structure of the toxin molecule did not significantly change after modification with PLP as revealed by the CD spectra, and the antigenicity of PLP derivative remained unchanged. The modified derivative retained its affinity for Ca2+, indicating that the modified Lys residues did not participate in Ca2+ binding. These results indicate that modification of Lys residues causes a differential effect on the enzymatic activity and lethal toxicity of notexin, and suggest that notexin might possess two functional sites, one responsible for the catalytic activity and the other associated with its lethal effect. More... »

PAGES

473-480

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01886855

DOI

http://dx.doi.org/10.1007/bf01886855

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025496613

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8895093


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anilino Naphthalenesulfonates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Elapid Venoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lethal Dose 50", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lysine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurotoxins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Secondary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pyridoxal Phosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Snakes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Structure-Activity Relationship", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, Kaohsiung Medical College, Kaohsiung, Taiwan 807, ROC", 
          "id": "http://www.grid.ac/institutes/grid.412019.f", 
          "name": [
            "Department of Biochemistry, Kaohsiung Medical College, Kaohsiung, Taiwan 807, ROC"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Long-Sen", 
        "id": "sg:person.0770353614.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01890463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029434261", 
          "https://doi.org/10.1007/bf01890463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01025581", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025292357", 
          "https://doi.org/10.1007/bf01025581"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01040500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016281114", 
          "https://doi.org/10.1007/bf01040500"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-07", 
    "datePublishedReg": "1996-07-01", 
    "description": "Notexin fromNotechis scutatus scutatus snake venom was subjected to Lys modification with pyridoxal 5\u2032-phosphate (PLP), and one major modified derivative was purified on a cation-exchanger SP-8HR column. The results of amino acid analysis and sequence determination revealed that only 2 Lys residues at positions 82 and 115 out of 11 Lys residues in notexin were modified. The incorporation of PLP into the protein was accompanied by the loss of 53% lethal toxicity, but the modified notexin showed an about 1.2-fold increase in enzymatic activity. However, the secondary structure of the toxin molecule did not significantly change after modification with PLP as revealed by the CD spectra, and the antigenicity of PLP derivative remained unchanged. The modified derivative retained its affinity for Ca2+, indicating that the modified Lys residues did not participate in Ca2+ binding. These results indicate that modification of Lys residues causes a differential effect on the enzymatic activity and lethal toxicity of notexin, and suggest that notexin might possess two functional sites, one responsible for the catalytic activity and the other associated with its lethal effect.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf01886855", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297375", 
        "issn": [
          "1572-3887", 
          "1573-4943"
        ], 
        "name": "The Protein Journal", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "keywords": [
      "Lys residues", 
      "catalytic activity", 
      "chemical modification", 
      "CD spectra", 
      "PLP derivatives", 
      "Lys modification", 
      "amino acid analysis", 
      "derivatives", 
      "secondary structure", 
      "toxin molecule", 
      "modification of Lys", 
      "enzymatic activity", 
      "incorporation of PLP", 
      "acid analysis", 
      "functional sites", 
      "modification", 
      "sequence determination", 
      "lethal toxicity", 
      "snake venom", 
      "molecules", 
      "residues", 
      "position 82", 
      "Lys", 
      "lethal effects", 
      "spectra", 
      "toxicity", 
      "column", 
      "affinity", 
      "venom", 
      "determination", 
      "incorporation", 
      "activity", 
      "PLP", 
      "structure", 
      "binding", 
      "protein", 
      "Ca2", 
      "notexin", 
      "differential effects", 
      "sites", 
      "effect", 
      "antigenicity", 
      "results", 
      "analysis", 
      "loss", 
      "increase"
    ], 
    "name": "Chemical modification of notexin fromNotechis scutatus scutatus (Australian tiger snake) venom with pyridoxal-5\u2032-phosphate", 
    "pagination": "473-480", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1025496613"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01886855"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8895093"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01886855", 
      "https://app.dimensions.ai/details/publication/pub.1025496613"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_292.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf01886855"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01886855'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01886855'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01886855'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01886855'


 

This table displays all metadata directly associated to this object as RDF triples.

183 TRIPLES      21 PREDICATES      91 URIs      80 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01886855 schema:about N000e6148f1d94fc097e9460c147151f9
2 N02f3333f04fb4b7fbbccdba38dd9ce08
3 N1ef6fc06a764490b944a30bb9209d66f
4 N2d43794fa04b44b695c2aa1d1aef6d04
5 N3207afb1a77640389e2dcd68573e8604
6 N41d23d14ac9d4dea9a10a1b22358f103
7 N55f895a264ff475b9a354f6eebc29715
8 N6d8aa330d0ec4fb585ab89fe3992d807
9 N77ada1d330104f0ca8553d42bedd23e2
10 N7d9131278e9b4350a9a6c41659da5090
11 N802345cc0c284a179032f5bdff2845e6
12 N858ff6ac130c4348a428f6712e51932f
13 N97c7c98159e14e848a6e26534eb673ba
14 N9a13a90cadaf41c98c0253ac054d3cc9
15 N9d4a1cf50211492c973919e24e892bda
16 Ne49d89c182a9439eaab6d7f243f72288
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Nda04bb9952ad4fc5b4e8481ad24c8798
20 schema:citation sg:pub.10.1007/bf01025581
21 sg:pub.10.1007/bf01040500
22 sg:pub.10.1007/bf01890463
23 schema:datePublished 1996-07
24 schema:datePublishedReg 1996-07-01
25 schema:description Notexin fromNotechis scutatus scutatus snake venom was subjected to Lys modification with pyridoxal 5′-phosphate (PLP), and one major modified derivative was purified on a cation-exchanger SP-8HR column. The results of amino acid analysis and sequence determination revealed that only 2 Lys residues at positions 82 and 115 out of 11 Lys residues in notexin were modified. The incorporation of PLP into the protein was accompanied by the loss of 53% lethal toxicity, but the modified notexin showed an about 1.2-fold increase in enzymatic activity. However, the secondary structure of the toxin molecule did not significantly change after modification with PLP as revealed by the CD spectra, and the antigenicity of PLP derivative remained unchanged. The modified derivative retained its affinity for Ca2+, indicating that the modified Lys residues did not participate in Ca2+ binding. These results indicate that modification of Lys residues causes a differential effect on the enzymatic activity and lethal toxicity of notexin, and suggest that notexin might possess two functional sites, one responsible for the catalytic activity and the other associated with its lethal effect.
26 schema:genre article
27 schema:isAccessibleForFree false
28 schema:isPartOf N12bb942e2fa4483eb5cd52d12995cfda
29 Nde76ebac5879452c9fc672f9912abc7c
30 sg:journal.1297375
31 schema:keywords CD spectra
32 Ca2
33 Lys
34 Lys modification
35 Lys residues
36 PLP
37 PLP derivatives
38 acid analysis
39 activity
40 affinity
41 amino acid analysis
42 analysis
43 antigenicity
44 binding
45 catalytic activity
46 chemical modification
47 column
48 derivatives
49 determination
50 differential effects
51 effect
52 enzymatic activity
53 functional sites
54 incorporation
55 incorporation of PLP
56 increase
57 lethal effects
58 lethal toxicity
59 loss
60 modification
61 modification of Lys
62 molecules
63 notexin
64 position 82
65 protein
66 residues
67 results
68 secondary structure
69 sequence determination
70 sites
71 snake venom
72 spectra
73 structure
74 toxicity
75 toxin molecule
76 venom
77 schema:name Chemical modification of notexin fromNotechis scutatus scutatus (Australian tiger snake) venom with pyridoxal-5′-phosphate
78 schema:pagination 473-480
79 schema:productId N1d46fa30ae774af5b16bae38e71834a1
80 N1e25a915be3841ea8638884a71ec79d9
81 N75eca7de7610411d9df3592955ff77fa
82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025496613
83 https://doi.org/10.1007/bf01886855
84 schema:sdDatePublished 2022-10-01T06:30
85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
86 schema:sdPublisher N9c68ced990bd41e2b36a751c0a82dac6
87 schema:url https://doi.org/10.1007/bf01886855
88 sgo:license sg:explorer/license/
89 sgo:sdDataset articles
90 rdf:type schema:ScholarlyArticle
91 N000e6148f1d94fc097e9460c147151f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Anilino Naphthalenesulfonates
93 rdf:type schema:DefinedTerm
94 N02f3333f04fb4b7fbbccdba38dd9ce08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Structure-Activity Relationship
96 rdf:type schema:DefinedTerm
97 N12bb942e2fa4483eb5cd52d12995cfda schema:issueNumber 5
98 rdf:type schema:PublicationIssue
99 N1d46fa30ae774af5b16bae38e71834a1 schema:name dimensions_id
100 schema:value pub.1025496613
101 rdf:type schema:PropertyValue
102 N1e25a915be3841ea8638884a71ec79d9 schema:name doi
103 schema:value 10.1007/bf01886855
104 rdf:type schema:PropertyValue
105 N1ef6fc06a764490b944a30bb9209d66f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Snakes
107 rdf:type schema:DefinedTerm
108 N2d43794fa04b44b695c2aa1d1aef6d04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Protein Structure, Tertiary
110 rdf:type schema:DefinedTerm
111 N3207afb1a77640389e2dcd68573e8604 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Lysine
113 rdf:type schema:DefinedTerm
114 N41d23d14ac9d4dea9a10a1b22358f103 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Fluorescent Dyes
116 rdf:type schema:DefinedTerm
117 N55f895a264ff475b9a354f6eebc29715 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Lethal Dose 50
119 rdf:type schema:DefinedTerm
120 N6d8aa330d0ec4fb585ab89fe3992d807 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Calcium
122 rdf:type schema:DefinedTerm
123 N75eca7de7610411d9df3592955ff77fa schema:name pubmed_id
124 schema:value 8895093
125 rdf:type schema:PropertyValue
126 N77ada1d330104f0ca8553d42bedd23e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Protein Structure, Secondary
128 rdf:type schema:DefinedTerm
129 N7d9131278e9b4350a9a6c41659da5090 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Neurotoxins
131 rdf:type schema:DefinedTerm
132 N802345cc0c284a179032f5bdff2845e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Molecular Sequence Data
134 rdf:type schema:DefinedTerm
135 N858ff6ac130c4348a428f6712e51932f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Elapid Venoms
137 rdf:type schema:DefinedTerm
138 N97c7c98159e14e848a6e26534eb673ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Spectrometry, Fluorescence
140 rdf:type schema:DefinedTerm
141 N9a13a90cadaf41c98c0253ac054d3cc9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Animals
143 rdf:type schema:DefinedTerm
144 N9c68ced990bd41e2b36a751c0a82dac6 schema:name Springer Nature - SN SciGraph project
145 rdf:type schema:Organization
146 N9d4a1cf50211492c973919e24e892bda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Amino Acid Sequence
148 rdf:type schema:DefinedTerm
149 Nda04bb9952ad4fc5b4e8481ad24c8798 rdf:first sg:person.0770353614.14
150 rdf:rest rdf:nil
151 Nde76ebac5879452c9fc672f9912abc7c schema:volumeNumber 15
152 rdf:type schema:PublicationVolume
153 Ne49d89c182a9439eaab6d7f243f72288 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Pyridoxal Phosphate
155 rdf:type schema:DefinedTerm
156 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
157 schema:name Biological Sciences
158 rdf:type schema:DefinedTerm
159 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biochemistry and Cell Biology
161 rdf:type schema:DefinedTerm
162 sg:journal.1297375 schema:issn 1572-3887
163 1573-4943
164 schema:name The Protein Journal
165 schema:publisher Springer Nature
166 rdf:type schema:Periodical
167 sg:person.0770353614.14 schema:affiliation grid-institutes:grid.412019.f
168 schema:familyName Chang
169 schema:givenName Long-Sen
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14
171 rdf:type schema:Person
172 sg:pub.10.1007/bf01025581 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025292357
173 https://doi.org/10.1007/bf01025581
174 rdf:type schema:CreativeWork
175 sg:pub.10.1007/bf01040500 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016281114
176 https://doi.org/10.1007/bf01040500
177 rdf:type schema:CreativeWork
178 sg:pub.10.1007/bf01890463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029434261
179 https://doi.org/10.1007/bf01890463
180 rdf:type schema:CreativeWork
181 grid-institutes:grid.412019.f schema:alternateName Department of Biochemistry, Kaohsiung Medical College, Kaohsiung, Taiwan 807, ROC
182 schema:name Department of Biochemistry, Kaohsiung Medical College, Kaohsiung, Taiwan 807, ROC
183 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...