Monoclonal antibodies to rat renal tissue: an approach to the immunohistological analysis of the nephron-collecting duct system, and ultra-structural localization ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-01

AUTHORS

T. Shimokama, T. Watanabe

ABSTRACT

To obtain more accurate information on the nephron-collecting duct system, monoclonal antibodies against renal tissue were prepared. BALB/c mice were immunized every two weeks with rat renal tissue, either cortex or medulla. Spleen cells were collected and fused with myeloma cells sensitive to hypoxanthine-aminopterin-thymidine medium. Hybrids were selected for production of antibodies by indirect immunofluorescence and cloned by the limiting dilution method. Tissue reactivity of the antibodies obtained was defined by immunofluorescence. The intracellular localization of antigenic determinants was ascertained by immunoelectron microscopy. The antibodies were classified into four major groups: (1) antibodies against proximal tubules; (2) antibodies against distal tubules and the loop of Henle; (3) antibodies against collecting duct system; and (4) antibodies against glomeruli. Using immunoelectron microscopy, various intracellular antigenic determinants were recognized, such as brush border, apical canaliculi, vacuolar apparatus, luminal and basolateral plasma membranes. The results obtained indicated that electron microscopy is indispensable for the immunohistological study of the nephroncollecting duct system. The observations help to understand morphological and functional diversity of the nephron-collecting duct system. More... »

PAGES

13-21

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01886503

DOI

http://dx.doi.org/10.1007/bf01886503

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002521881

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1938467


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Antibody Technique", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoenzyme Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Tubules, Collecting", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan", 
          "id": "http://www.grid.ac/institutes/grid.412339.e", 
          "name": [
            "Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shimokama", 
        "givenName": "T.", 
        "id": "sg:person.01271015273.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271015273.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan", 
          "id": "http://www.grid.ac/institutes/grid.412339.e", 
          "name": [
            "Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "T.", 
        "id": "sg:person.012664624074.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00713375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015723455", 
          "https://doi.org/10.1007/bf00713375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/256495a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001387921", 
          "https://doi.org/10.1038/256495a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-67147-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1109710474", 
          "https://doi.org/10.1007/978-3-642-67147-0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1991-01", 
    "datePublishedReg": "1991-01-01", 
    "description": "To obtain more accurate information on the nephron-collecting duct system, monoclonal antibodies against renal tissue were prepared. BALB/c mice were immunized every two weeks with rat renal tissue, either cortex or medulla. Spleen cells were collected and fused with myeloma cells sensitive to hypoxanthine-aminopterin-thymidine medium. Hybrids were selected for production of antibodies by indirect immunofluorescence and cloned by the limiting dilution method. Tissue reactivity of the antibodies obtained was defined by immunofluorescence. The intracellular localization of antigenic determinants was ascertained by immunoelectron microscopy. The antibodies were classified into four major groups: (1) antibodies against proximal tubules; (2) antibodies against distal tubules and the loop of Henle; (3) antibodies against collecting duct system; and (4) antibodies against glomeruli. Using immunoelectron microscopy, various intracellular antigenic determinants were recognized, such as brush border, apical canaliculi, vacuolar apparatus, luminal and basolateral plasma membranes. The results obtained indicated that electron microscopy is indispensable for the immunohistological study of the nephroncollecting duct system. The observations help to understand morphological and functional diversity of the nephron-collecting duct system.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf01886503", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1032970", 
        "issn": [
          "1567-2379", 
          "1573-6865"
        ], 
        "name": "Journal of Molecular Histology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "23"
      }
    ], 
    "keywords": [
      "renal tissue", 
      "BALB/c mice", 
      "monoclonal antibodies", 
      "duct system", 
      "antigenic determinants", 
      "rat renal tissue", 
      "loop of Henle", 
      "production of antibodies", 
      "c mice", 
      "immunoelectron microscopy", 
      "immunohistological study", 
      "spleen cells", 
      "distal tubules", 
      "proximal tubules", 
      "immunohistological analysis", 
      "myeloma cells", 
      "indirect immunofluorescence", 
      "antibodies", 
      "tissue reactivity", 
      "nephron", 
      "brush border", 
      "tissue", 
      "dilution method", 
      "immunofluorescence", 
      "tubules", 
      "intracellular localization", 
      "cells", 
      "ultra-structural localization", 
      "basolateral plasma membrane", 
      "medulla", 
      "glomeruli", 
      "cortex", 
      "antigen", 
      "mice", 
      "Henle", 
      "weeks", 
      "vacuolar apparatus", 
      "determinants", 
      "thymidine medium", 
      "apical canaliculi", 
      "canaliculi", 
      "plasma membrane", 
      "electron microscopy", 
      "accurate information", 
      "localization", 
      "group", 
      "microscopy", 
      "major groups", 
      "study", 
      "reactivity", 
      "membrane", 
      "border", 
      "analysis", 
      "production", 
      "system", 
      "results", 
      "information", 
      "observations", 
      "hybrids", 
      "method", 
      "medium", 
      "approach", 
      "apparatus", 
      "loop", 
      "functional diversity", 
      "diversity"
    ], 
    "name": "Monoclonal antibodies to rat renal tissue: an approach to the immunohistological analysis of the nephron-collecting duct system, and ultra-structural localization of antigens", 
    "pagination": "13-21", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002521881"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01886503"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1938467"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01886503", 
      "https://app.dimensions.ai/details/publication/pub.1002521881"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_253.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf01886503"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01886503'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01886503'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01886503'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01886503'


 

This table displays all metadata directly associated to this object as RDF triples.

178 TRIPLES      21 PREDICATES      103 URIs      92 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01886503 schema:about N46413ad1a454434b9d8954b8da003169
2 N4a7b3e8d1c9548118e8d66bea898580d
3 N54c86ff15e7d4a9b9e1be24a936c6cc4
4 N63a77c25077f4d10a597cff7b3ce4787
5 Na7112f7e15e24a73af2a20e0078e26b0
6 Nd225b252b9f0497ebe7171435b8b6cd0
7 Nddcd73bc69544b49a8138699e8a7c0f2
8 Ne9f9a7afe93c4b55b02f6520e9d6cd23
9 anzsrc-for:11
10 anzsrc-for:1107
11 schema:author N2ae33bd4c1144fa79d1cf36a6de8eb7c
12 schema:citation sg:pub.10.1007/978-3-642-67147-0
13 sg:pub.10.1007/bf00713375
14 sg:pub.10.1038/256495a0
15 schema:datePublished 1991-01
16 schema:datePublishedReg 1991-01-01
17 schema:description To obtain more accurate information on the nephron-collecting duct system, monoclonal antibodies against renal tissue were prepared. BALB/c mice were immunized every two weeks with rat renal tissue, either cortex or medulla. Spleen cells were collected and fused with myeloma cells sensitive to hypoxanthine-aminopterin-thymidine medium. Hybrids were selected for production of antibodies by indirect immunofluorescence and cloned by the limiting dilution method. Tissue reactivity of the antibodies obtained was defined by immunofluorescence. The intracellular localization of antigenic determinants was ascertained by immunoelectron microscopy. The antibodies were classified into four major groups: (1) antibodies against proximal tubules; (2) antibodies against distal tubules and the loop of Henle; (3) antibodies against collecting duct system; and (4) antibodies against glomeruli. Using immunoelectron microscopy, various intracellular antigenic determinants were recognized, such as brush border, apical canaliculi, vacuolar apparatus, luminal and basolateral plasma membranes. The results obtained indicated that electron microscopy is indispensable for the immunohistological study of the nephroncollecting duct system. The observations help to understand morphological and functional diversity of the nephron-collecting duct system.
18 schema:genre article
19 schema:isAccessibleForFree false
20 schema:isPartOf N5b1b609ad0ac4bc7b873d1147e197163
21 N7bb2059e787b41a785c0efddcc13691a
22 sg:journal.1032970
23 schema:keywords BALB/c mice
24 Henle
25 accurate information
26 analysis
27 antibodies
28 antigen
29 antigenic determinants
30 apical canaliculi
31 apparatus
32 approach
33 basolateral plasma membrane
34 border
35 brush border
36 c mice
37 canaliculi
38 cells
39 cortex
40 determinants
41 dilution method
42 distal tubules
43 diversity
44 duct system
45 electron microscopy
46 functional diversity
47 glomeruli
48 group
49 hybrids
50 immunoelectron microscopy
51 immunofluorescence
52 immunohistological analysis
53 immunohistological study
54 indirect immunofluorescence
55 information
56 intracellular localization
57 localization
58 loop
59 loop of Henle
60 major groups
61 medium
62 medulla
63 membrane
64 method
65 mice
66 microscopy
67 monoclonal antibodies
68 myeloma cells
69 nephron
70 observations
71 plasma membrane
72 production
73 production of antibodies
74 proximal tubules
75 rat renal tissue
76 reactivity
77 renal tissue
78 results
79 spleen cells
80 study
81 system
82 thymidine medium
83 tissue
84 tissue reactivity
85 tubules
86 ultra-structural localization
87 vacuolar apparatus
88 weeks
89 schema:name Monoclonal antibodies to rat renal tissue: an approach to the immunohistological analysis of the nephron-collecting duct system, and ultra-structural localization of antigens
90 schema:pagination 13-21
91 schema:productId N3cb7ca22197c42358f0bf19725f95075
92 N84f299c24c6a43b8ab9b962353e7fd12
93 Nd1c1f21bb7f34c398feb993046677a4a
94 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002521881
95 https://doi.org/10.1007/bf01886503
96 schema:sdDatePublished 2022-08-04T16:52
97 schema:sdLicense https://scigraph.springernature.com/explorer/license/
98 schema:sdPublisher N2b77871aee0d4fd08d4b2ad7d00d7d78
99 schema:url https://doi.org/10.1007/bf01886503
100 sgo:license sg:explorer/license/
101 sgo:sdDataset articles
102 rdf:type schema:ScholarlyArticle
103 N2ae33bd4c1144fa79d1cf36a6de8eb7c rdf:first sg:person.01271015273.11
104 rdf:rest Nef3efa5032a44e7b83a37c34752354a9
105 N2b77871aee0d4fd08d4b2ad7d00d7d78 schema:name Springer Nature - SN SciGraph project
106 rdf:type schema:Organization
107 N3cb7ca22197c42358f0bf19725f95075 schema:name dimensions_id
108 schema:value pub.1002521881
109 rdf:type schema:PropertyValue
110 N46413ad1a454434b9d8954b8da003169 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Mice
112 rdf:type schema:DefinedTerm
113 N4a7b3e8d1c9548118e8d66bea898580d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Kidney Tubules, Collecting
115 rdf:type schema:DefinedTerm
116 N54c86ff15e7d4a9b9e1be24a936c6cc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Fluorescent Antibody Technique
118 rdf:type schema:DefinedTerm
119 N5b1b609ad0ac4bc7b873d1147e197163 schema:issueNumber 1
120 rdf:type schema:PublicationIssue
121 N63a77c25077f4d10a597cff7b3ce4787 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Rats
123 rdf:type schema:DefinedTerm
124 N7bb2059e787b41a785c0efddcc13691a schema:volumeNumber 23
125 rdf:type schema:PublicationVolume
126 N84f299c24c6a43b8ab9b962353e7fd12 schema:name pubmed_id
127 schema:value 1938467
128 rdf:type schema:PropertyValue
129 Na7112f7e15e24a73af2a20e0078e26b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Antibodies, Monoclonal
131 rdf:type schema:DefinedTerm
132 Nd1c1f21bb7f34c398feb993046677a4a schema:name doi
133 schema:value 10.1007/bf01886503
134 rdf:type schema:PropertyValue
135 Nd225b252b9f0497ebe7171435b8b6cd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Immunoenzyme Techniques
137 rdf:type schema:DefinedTerm
138 Nddcd73bc69544b49a8138699e8a7c0f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Animals
140 rdf:type schema:DefinedTerm
141 Ne9f9a7afe93c4b55b02f6520e9d6cd23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Antigens
143 rdf:type schema:DefinedTerm
144 Nef3efa5032a44e7b83a37c34752354a9 rdf:first sg:person.012664624074.29
145 rdf:rest rdf:nil
146 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
147 schema:name Medical and Health Sciences
148 rdf:type schema:DefinedTerm
149 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
150 schema:name Immunology
151 rdf:type schema:DefinedTerm
152 sg:journal.1032970 schema:issn 1567-2379
153 1573-6865
154 schema:name Journal of Molecular Histology
155 schema:publisher Springer Nature
156 rdf:type schema:Periodical
157 sg:person.012664624074.29 schema:affiliation grid-institutes:grid.412339.e
158 schema:familyName Watanabe
159 schema:givenName T.
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29
161 rdf:type schema:Person
162 sg:person.01271015273.11 schema:affiliation grid-institutes:grid.412339.e
163 schema:familyName Shimokama
164 schema:givenName T.
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271015273.11
166 rdf:type schema:Person
167 sg:pub.10.1007/978-3-642-67147-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1109710474
168 https://doi.org/10.1007/978-3-642-67147-0
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/bf00713375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015723455
171 https://doi.org/10.1007/bf00713375
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/256495a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001387921
174 https://doi.org/10.1038/256495a0
175 rdf:type schema:CreativeWork
176 grid-institutes:grid.412339.e schema:alternateName Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan
177 schema:name Department of Pathology, Saga Medical School, Nabeshima, 849, Saga, Japan
178 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...