Some properties of Bomirski Ab amelanotic melanoma cells, which underwent spontaneous melanization in primary cell culture View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-02

AUTHORS

Andrzej Słomiński

ABSTRACT

Four types of the Bomirski Ab amelanotic melanoma primary cell culture, differing in the presence of calf serum in the medium and in the cell number used for starting the culture, were employed in the study. In all types of cell culture, rapid melanization occurred in the cytoplasm of the cultured cells. Calf serum in the culture medium stimulated both melanization and proliferation of the Ab melanoma cells. The process of melanin synthesis occurred during the logarithmic phase of growth and was over when the cells reached the plateau phase. Heavily melanized cells changed their adhesive properties, lost the ability to divide in vitro, and showed decreasing tumorigenicity down to complete absence, though they retained some parameters of viability. The rate of melanin synthesis was lower in the cells cultured at high cell density than in those at low cell density. Highly melanized cells that did not divide in vitro but were still tumorigenic in vivo caused the growth of tumors those morphology was typical for the amelanotic melanoma, melanin being absent. In conclusion, it may be stated that the present findings suggest the persistence of a highly anaplastic and malignant phenotype of Bomirski amelanotic melanoma, being a result of the regulatory action of the host, while the change in the phenotype in vitro does not rule out autoregulatory influences of the tumor itself on its differentiation level and malignancy. More... »

PAGES

29-37

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01884251

DOI

http://dx.doi.org/10.1007/bf01884251

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048433590

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3972882


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Count", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Transformation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histocytochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melanins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melanoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesocricetus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Transplantation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, Institute of Medical Biology, Medical School, ul. Debinki 1, 80-211, Gda\u0144sk, Poland", 
          "id": "http://www.grid.ac/institutes/grid.453758.8", 
          "name": [
            "Department of Biology, Institute of Medical Biology, Medical School, ul. Debinki 1, 80-211, Gda\u0144sk, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "S\u0142omi\u0144ski", 
        "givenName": "Andrzej", 
        "id": "sg:person.016612034677.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016612034677.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02138331", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041915802", 
          "https://doi.org/10.1007/bf02138331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00595427", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000639605", 
          "https://doi.org/10.1007/bf00595427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01121951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037626259", 
          "https://doi.org/10.1007/bf01121951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02618156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010345412", 
          "https://doi.org/10.1007/bf02618156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-99906-2_29", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040042201", 
          "https://doi.org/10.1007/978-3-642-99906-2_29"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1985-02", 
    "datePublishedReg": "1985-02-01", 
    "description": "Four types of the Bomirski Ab amelanotic melanoma primary cell culture, differing in the presence of calf serum in the medium and in the cell number used for starting the culture, were employed in the study. In all types of cell culture, rapid melanization occurred in the cytoplasm of the cultured cells. Calf serum in the culture medium stimulated both melanization and proliferation of the Ab melanoma cells. The process of melanin synthesis occurred during the logarithmic phase of growth and was over when the cells reached the plateau phase. Heavily melanized cells changed their adhesive properties, lost the ability to divide in vitro, and showed decreasing tumorigenicity down to complete absence, though they retained some parameters of viability. The rate of melanin synthesis was lower in the cells cultured at high cell density than in those at low cell density. Highly melanized cells that did not divide in vitro but were still tumorigenic in vivo caused the growth of tumors those morphology was typical for the amelanotic melanoma, melanin being absent. In conclusion, it may be stated that the present findings suggest the persistence of a highly anaplastic and malignant phenotype of Bomirski amelanotic melanoma, being a result of the regulatory action of the host, while the change in the phenotype in vitro does not rule out autoregulatory influences of the tumor itself on its differentiation level and malignancy.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf01884251", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1313647", 
        "issn": [
          "0171-5216", 
          "1432-1335"
        ], 
        "name": "Journal of Cancer Research and Clinical Oncology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "109"
      }
    ], 
    "keywords": [
      "primary cell cultures", 
      "melanin synthesis", 
      "cell cultures", 
      "melanoma cells", 
      "Ab melanoma cells", 
      "spontaneous melanization", 
      "low cell density", 
      "rapid melanization", 
      "high cell density", 
      "cell density", 
      "amelanotic melanoma cells", 
      "cultured cells", 
      "malignant phenotype", 
      "melanization", 
      "calf serum", 
      "logarithmic phase", 
      "melanized cells", 
      "differentiation level", 
      "cell number", 
      "cells", 
      "phenotype", 
      "complete absence", 
      "autoregulatory influences", 
      "culture medium", 
      "growth of tumors", 
      "parameters of viability", 
      "vitro", 
      "cytoplasm", 
      "regulatory action", 
      "growth", 
      "adhesive properties", 
      "tumorigenicity", 
      "culture", 
      "host", 
      "proliferation", 
      "melanin", 
      "vivo", 
      "viability", 
      "synthesis", 
      "persistence", 
      "present findings", 
      "melanoma", 
      "absence", 
      "medium", 
      "types", 
      "plateau phase", 
      "morphology", 
      "ability", 
      "amelanotic melanoma", 
      "tumors", 
      "presence", 
      "levels", 
      "action", 
      "changes", 
      "density", 
      "number", 
      "serum", 
      "process", 
      "findings", 
      "study", 
      "malignancy", 
      "results", 
      "phase", 
      "rate", 
      "properties", 
      "influence", 
      "conclusion", 
      "parameters"
    ], 
    "name": "Some properties of Bomirski Ab amelanotic melanoma cells, which underwent spontaneous melanization in primary cell culture", 
    "pagination": "29-37", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048433590"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01884251"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "3972882"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01884251", 
      "https://app.dimensions.ai/details/publication/pub.1048433590"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_196.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf01884251"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01884251'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01884251'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01884251'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01884251'


 

This table displays all metadata directly associated to this object as RDF triples.

205 TRIPLES      21 PREDICATES      113 URIs      100 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01884251 schema:about N0721650d2e2b4d89b10085d1bcff697f
2 N08f27dde8af34b8ba1141cd3aa59a3cf
3 N090af97c06e34540bec747e8334f0ae2
4 N15ec48751d5443dcb047e7b1af343210
5 N165f1f801e1d403992247bb30f40ec3c
6 N2d20e315d9b84ddb8c83e70fc7195410
7 N54b4d14a04cd44e7b1812cfb8440d4a0
8 N6139587a41a24832829a0d0228e02060
9 N65010359486b4b8d817dc2d50f7e0348
10 N79a56db7d8b94bb487625bb6d947af21
11 Na6010352455549539c858f89921be747
12 Nb147d537548143c99636b21217f2a873
13 Nb9c3ff7377764521b428dcca8fdf019c
14 Nbfee3c7b6fa44a66a19e41e780652028
15 anzsrc-for:11
16 anzsrc-for:1112
17 schema:author Ncf16afd8b75743608a8c4172dd1a00ae
18 schema:citation sg:pub.10.1007/978-3-642-99906-2_29
19 sg:pub.10.1007/bf00595427
20 sg:pub.10.1007/bf01121951
21 sg:pub.10.1007/bf02138331
22 sg:pub.10.1007/bf02618156
23 schema:datePublished 1985-02
24 schema:datePublishedReg 1985-02-01
25 schema:description Four types of the Bomirski Ab amelanotic melanoma primary cell culture, differing in the presence of calf serum in the medium and in the cell number used for starting the culture, were employed in the study. In all types of cell culture, rapid melanization occurred in the cytoplasm of the cultured cells. Calf serum in the culture medium stimulated both melanization and proliferation of the Ab melanoma cells. The process of melanin synthesis occurred during the logarithmic phase of growth and was over when the cells reached the plateau phase. Heavily melanized cells changed their adhesive properties, lost the ability to divide in vitro, and showed decreasing tumorigenicity down to complete absence, though they retained some parameters of viability. The rate of melanin synthesis was lower in the cells cultured at high cell density than in those at low cell density. Highly melanized cells that did not divide in vitro but were still tumorigenic in vivo caused the growth of tumors those morphology was typical for the amelanotic melanoma, melanin being absent. In conclusion, it may be stated that the present findings suggest the persistence of a highly anaplastic and malignant phenotype of Bomirski amelanotic melanoma, being a result of the regulatory action of the host, while the change in the phenotype in vitro does not rule out autoregulatory influences of the tumor itself on its differentiation level and malignancy.
26 schema:genre article
27 schema:isAccessibleForFree false
28 schema:isPartOf N6556a1552f0942c2825945797389d76e
29 N672271055c2c4157ae8fa16cdcb68d0f
30 sg:journal.1313647
31 schema:keywords Ab melanoma cells
32 ability
33 absence
34 action
35 adhesive properties
36 amelanotic melanoma
37 amelanotic melanoma cells
38 autoregulatory influences
39 calf serum
40 cell cultures
41 cell density
42 cell number
43 cells
44 changes
45 complete absence
46 conclusion
47 culture
48 culture medium
49 cultured cells
50 cytoplasm
51 density
52 differentiation level
53 findings
54 growth
55 growth of tumors
56 high cell density
57 host
58 influence
59 levels
60 logarithmic phase
61 low cell density
62 malignancy
63 malignant phenotype
64 medium
65 melanin
66 melanin synthesis
67 melanization
68 melanized cells
69 melanoma
70 melanoma cells
71 morphology
72 number
73 parameters
74 parameters of viability
75 persistence
76 phase
77 phenotype
78 plateau phase
79 presence
80 present findings
81 primary cell cultures
82 process
83 proliferation
84 properties
85 rapid melanization
86 rate
87 regulatory action
88 results
89 serum
90 spontaneous melanization
91 study
92 synthesis
93 tumorigenicity
94 tumors
95 types
96 viability
97 vitro
98 vivo
99 schema:name Some properties of Bomirski Ab amelanotic melanoma cells, which underwent spontaneous melanization in primary cell culture
100 schema:pagination 29-37
101 schema:productId N4ffa828fb9704c7f81bdd446e23accb3
102 Na4abf8acc2384a629e1eeafc96318b05
103 Ne026f0358a064937a62861c8caa31eae
104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048433590
105 https://doi.org/10.1007/bf01884251
106 schema:sdDatePublished 2022-09-02T15:46
107 schema:sdLicense https://scigraph.springernature.com/explorer/license/
108 schema:sdPublisher Nd2ef0eb0a4c44fb9a63f5f4f2a56c5b6
109 schema:url https://doi.org/10.1007/bf01884251
110 sgo:license sg:explorer/license/
111 sgo:sdDataset articles
112 rdf:type schema:ScholarlyArticle
113 N0721650d2e2b4d89b10085d1bcff697f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Cricetinae
115 rdf:type schema:DefinedTerm
116 N08f27dde8af34b8ba1141cd3aa59a3cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Histocytochemistry
118 rdf:type schema:DefinedTerm
119 N090af97c06e34540bec747e8334f0ae2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Cell Line
121 rdf:type schema:DefinedTerm
122 N15ec48751d5443dcb047e7b1af343210 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Neoplasm Transplantation
124 rdf:type schema:DefinedTerm
125 N165f1f801e1d403992247bb30f40ec3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Melanins
127 rdf:type schema:DefinedTerm
128 N2d20e315d9b84ddb8c83e70fc7195410 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Mesocricetus
130 rdf:type schema:DefinedTerm
131 N4ffa828fb9704c7f81bdd446e23accb3 schema:name pubmed_id
132 schema:value 3972882
133 rdf:type schema:PropertyValue
134 N54b4d14a04cd44e7b1812cfb8440d4a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Male
136 rdf:type schema:DefinedTerm
137 N6139587a41a24832829a0d0228e02060 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Cell Division
139 rdf:type schema:DefinedTerm
140 N65010359486b4b8d817dc2d50f7e0348 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Cells, Cultured
142 rdf:type schema:DefinedTerm
143 N6556a1552f0942c2825945797389d76e schema:volumeNumber 109
144 rdf:type schema:PublicationVolume
145 N672271055c2c4157ae8fa16cdcb68d0f schema:issueNumber 1
146 rdf:type schema:PublicationIssue
147 N79a56db7d8b94bb487625bb6d947af21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Animals
149 rdf:type schema:DefinedTerm
150 Na4abf8acc2384a629e1eeafc96318b05 schema:name dimensions_id
151 schema:value pub.1048433590
152 rdf:type schema:PropertyValue
153 Na6010352455549539c858f89921be747 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Cell Survival
155 rdf:type schema:DefinedTerm
156 Nb147d537548143c99636b21217f2a873 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Cell Transformation, Neoplastic
158 rdf:type schema:DefinedTerm
159 Nb9c3ff7377764521b428dcca8fdf019c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Melanoma
161 rdf:type schema:DefinedTerm
162 Nbfee3c7b6fa44a66a19e41e780652028 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Cell Count
164 rdf:type schema:DefinedTerm
165 Ncf16afd8b75743608a8c4172dd1a00ae rdf:first sg:person.016612034677.24
166 rdf:rest rdf:nil
167 Nd2ef0eb0a4c44fb9a63f5f4f2a56c5b6 schema:name Springer Nature - SN SciGraph project
168 rdf:type schema:Organization
169 Ne026f0358a064937a62861c8caa31eae schema:name doi
170 schema:value 10.1007/bf01884251
171 rdf:type schema:PropertyValue
172 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
173 schema:name Medical and Health Sciences
174 rdf:type schema:DefinedTerm
175 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
176 schema:name Oncology and Carcinogenesis
177 rdf:type schema:DefinedTerm
178 sg:journal.1313647 schema:issn 0171-5216
179 1432-1335
180 schema:name Journal of Cancer Research and Clinical Oncology
181 schema:publisher Springer Nature
182 rdf:type schema:Periodical
183 sg:person.016612034677.24 schema:affiliation grid-institutes:grid.453758.8
184 schema:familyName Słomiński
185 schema:givenName Andrzej
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016612034677.24
187 rdf:type schema:Person
188 sg:pub.10.1007/978-3-642-99906-2_29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040042201
189 https://doi.org/10.1007/978-3-642-99906-2_29
190 rdf:type schema:CreativeWork
191 sg:pub.10.1007/bf00595427 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000639605
192 https://doi.org/10.1007/bf00595427
193 rdf:type schema:CreativeWork
194 sg:pub.10.1007/bf01121951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037626259
195 https://doi.org/10.1007/bf01121951
196 rdf:type schema:CreativeWork
197 sg:pub.10.1007/bf02138331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041915802
198 https://doi.org/10.1007/bf02138331
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/bf02618156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010345412
201 https://doi.org/10.1007/bf02618156
202 rdf:type schema:CreativeWork
203 grid-institutes:grid.453758.8 schema:alternateName Department of Biology, Institute of Medical Biology, Medical School, ul. Debinki 1, 80-211, Gdańsk, Poland
204 schema:name Department of Biology, Institute of Medical Biology, Medical School, ul. Debinki 1, 80-211, Gdańsk, Poland
205 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...