Expression of the Tn antigen on erythroid cells from a patient with Tn syndrome View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1992-12

AUTHORS

Yoko Kamitani, Eiji Kajii, Toshio Suda, Shigenori Ikemoto

ABSTRACT

In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy volunteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells. More... »

PAGES

271-283

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01883318

DOI

http://dx.doi.org/10.1007/bf01883318

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039912340

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1297447


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