Ionophoric material derived from eel membrane preparation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-12

AUTHORS

Adil E. Shamoo, Marjory M. Myers, Robert Blumenthal, R. Wayne Albers

ABSTRACT

Prolonged digestion with trypsin released ionophoric material from a preparation of microsomes obtained fromElectrophorus electricus. The material induced a large increase in the conductance of a black lipid membrane prepared from oxidized cholesterol. The induced conductance of cations was greater than that of anions. There was no discrimination between monovalent inorganic cations, neither with respect to conductance nor with respect to rate of incorporation. Gel filtration indicated a molecular weight of about 2000 for the active material. The material was 50- to 200-fold more effective when applied to the membrane on both sides than when applied on only one side. The material was unstable to storage, particularly at pH levels far from enutrality, but was partially reactivated in some cases by lyophilization or by a high concentration of dithiothereitol. More... »

PAGES

129-140

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01869974

DOI

http://dx.doi.org/10.1007/bf01869974

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036902151

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4431039


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