Different locations of carbohydrate-containing sites in the surface membrane of normal and transformed mammalian cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1970-12

AUTHORS

Ben-Ami Sela, Halina Lis, Nathan Sharon, Leo Sachs

ABSTRACT

A soybean agglutinin was found to agglutinate mouse, rat and human cell lines transformed by viral carcinogens, but not hamster cells transformed by viral or non-viral carcinogens. Normal cells from which the transformed cells were derived were not agglutinated by this agglutinin, but they were rendered agglutinable after short incubation with trypsin or pronase. The transformed hamster cells, on the other hand, became agglutinable only after prolonged treatment with pronase. The agglutination was specifically inhibited by N-acetyl-d-galactosamine, indicating that N-acetyl-d-galactosamine-like saccharides are part of the receptor sites for soybean agglutinin on the surface membrane. Such sites exist in a cryptic form in normal cells; they are exposed in transformed mouse, rat and human cells, but become less accessible in transformed hamster cells. The receptor sites for soybean agglutinin differ from the receptors for two other plant agglutinins (wheat germ agglutinin that interacts with N-acetyl-d-glucosamine-like sites and Concanavalin A that interacts with α-d-glucopyranoside-like sites) which become exposed upon transformation of all lines tested. In normal hamster cells, the receptors for all three agglutinins become exposed after incubation with trypsin, but the exposure of N-acetyl-d-galactosamine-like sites requires the longest enzyme treatment. The results indicate a difference in the location of different carbohydrate-containing sites in the surface membrane. The differences in the exposure of carbohydrate-containing sites in the membrane could not be correlated with the levels of carbohydrate-splitting glycosidases in normal and transformed cells. More... »

PAGES

267-279

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01868019

DOI

http://dx.doi.org/10.1007/bf01868019

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046508734

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24174197


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel", 
          "id": "http://www.grid.ac/institutes/grid.13992.30", 
          "name": [
            "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sela", 
        "givenName": "Ben-Ami", 
        "id": "sg:person.0614161163.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614161163.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel", 
          "id": "http://www.grid.ac/institutes/grid.13992.30", 
          "name": [
            "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lis", 
        "givenName": "Halina", 
        "id": "sg:person.013464700163.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013464700163.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel", 
          "id": "http://www.grid.ac/institutes/grid.13992.30", 
          "name": [
            "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharon", 
        "givenName": "Nathan", 
        "id": "sg:person.01315713223.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315713223.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel", 
          "id": "http://www.grid.ac/institutes/grid.13992.30", 
          "name": [
            "Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sachs", 
        "givenName": "Leo", 
        "id": "sg:person.047342437.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047342437.65"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/2071272a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020383449", 
          "https://doi.org/10.1038/2071272a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/223710a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000989241", 
          "https://doi.org/10.1038/223710a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1970-12", 
    "datePublishedReg": "1970-12-01", 
    "description": "A soybean agglutinin was found to agglutinate mouse, rat and human cell lines transformed by viral carcinogens, but not hamster cells transformed by viral or non-viral carcinogens. Normal cells from which the transformed cells were derived were not agglutinated by this agglutinin, but they were rendered agglutinable after short incubation with trypsin or pronase. The transformed hamster cells, on the other hand, became agglutinable only after prolonged treatment with pronase. The agglutination was specifically inhibited by N-acetyl-d-galactosamine, indicating that N-acetyl-d-galactosamine-like saccharides are part of the receptor sites for soybean agglutinin on the surface membrane. Such sites exist in a cryptic form in normal cells; they are exposed in transformed mouse, rat and human cells, but become less accessible in transformed hamster cells. The receptor sites for soybean agglutinin differ from the receptors for two other plant agglutinins (wheat germ agglutinin that interacts with N-acetyl-d-glucosamine-like sites and Concanavalin A that interacts with \u03b1-d-glucopyranoside-like sites) which become exposed upon transformation of all lines tested. In normal hamster cells, the receptors for all three agglutinins become exposed after incubation with trypsin, but the exposure of N-acetyl-d-galactosamine-like sites requires the longest enzyme treatment. The results indicate a difference in the location of different carbohydrate-containing sites in the surface membrane. The differences in the exposure of carbohydrate-containing sites in the membrane could not be correlated with the levels of carbohydrate-splitting glycosidases in normal and transformed cells.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf01868019", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1012006", 
        "issn": [
          "0022-2631", 
          "1432-1424"
        ], 
        "name": "The Journal of Membrane Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "carbohydrate-containing sites", 
      "receptor sites", 
      "normal cells", 
      "surface membrane", 
      "soybean agglutinin", 
      "hamster cells", 
      "viral carcinogens", 
      "normal hamster cells", 
      "human cell lines", 
      "cell lines", 
      "rats", 
      "mice", 
      "receptors", 
      "agglutinin", 
      "cells", 
      "carcinogens", 
      "treatment", 
      "short incubation", 
      "exposure", 
      "human cells", 
      "acetyl", 
      "incubation", 
      "pronase", 
      "differences", 
      "galactosamine", 
      "agglutination", 
      "trypsin", 
      "cryptic form", 
      "enzyme treatment", 
      "membrane", 
      "mammalian cells", 
      "sites", 
      "levels", 
      "lines", 
      "plant agglutinins", 
      "location", 
      "glycosidases", 
      "hand", 
      "results", 
      "part", 
      "form", 
      "such sites", 
      "different locations", 
      "saccharides", 
      "transformation", 
      "non-viral carcinogens", 
      "galactosamine-like saccharides", 
      "galactosamine-like sites", 
      "longest enzyme treatment", 
      "different carbohydrate-containing sites", 
      "carbohydrate-splitting glycosidases"
    ], 
    "name": "Different locations of carbohydrate-containing sites in the surface membrane of normal and transformed mammalian cells", 
    "pagination": "267-279", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046508734"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01868019"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24174197"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01868019", 
      "https://app.dimensions.ai/details/publication/pub.1046508734"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_118.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf01868019"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01868019'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01868019'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01868019'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01868019'


 

This table displays all metadata directly associated to this object as RDF triples.

142 TRIPLES      22 PREDICATES      80 URIs      70 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01868019 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N061ec00c9b2543fcbdb3d933ccd06e1f
4 schema:citation sg:pub.10.1038/2071272a0
5 sg:pub.10.1038/223710a0
6 schema:datePublished 1970-12
7 schema:datePublishedReg 1970-12-01
8 schema:description A soybean agglutinin was found to agglutinate mouse, rat and human cell lines transformed by viral carcinogens, but not hamster cells transformed by viral or non-viral carcinogens. Normal cells from which the transformed cells were derived were not agglutinated by this agglutinin, but they were rendered agglutinable after short incubation with trypsin or pronase. The transformed hamster cells, on the other hand, became agglutinable only after prolonged treatment with pronase. The agglutination was specifically inhibited by N-acetyl-d-galactosamine, indicating that N-acetyl-d-galactosamine-like saccharides are part of the receptor sites for soybean agglutinin on the surface membrane. Such sites exist in a cryptic form in normal cells; they are exposed in transformed mouse, rat and human cells, but become less accessible in transformed hamster cells. The receptor sites for soybean agglutinin differ from the receptors for two other plant agglutinins (wheat germ agglutinin that interacts with N-acetyl-d-glucosamine-like sites and Concanavalin A that interacts with α-d-glucopyranoside-like sites) which become exposed upon transformation of all lines tested. In normal hamster cells, the receptors for all three agglutinins become exposed after incubation with trypsin, but the exposure of N-acetyl-d-galactosamine-like sites requires the longest enzyme treatment. The results indicate a difference in the location of different carbohydrate-containing sites in the surface membrane. The differences in the exposure of carbohydrate-containing sites in the membrane could not be correlated with the levels of carbohydrate-splitting glycosidases in normal and transformed cells.
9 schema:genre article
10 schema:inLanguage en
11 schema:isAccessibleForFree false
12 schema:isPartOf N8dd09d3f0cd0461d9d5b31dacc8d45ed
13 Nefe60a9549b648d9af90d8487724acc8
14 sg:journal.1012006
15 schema:keywords acetyl
16 agglutination
17 agglutinin
18 carbohydrate-containing sites
19 carbohydrate-splitting glycosidases
20 carcinogens
21 cell lines
22 cells
23 cryptic form
24 differences
25 different carbohydrate-containing sites
26 different locations
27 enzyme treatment
28 exposure
29 form
30 galactosamine
31 galactosamine-like saccharides
32 galactosamine-like sites
33 glycosidases
34 hamster cells
35 hand
36 human cell lines
37 human cells
38 incubation
39 levels
40 lines
41 location
42 longest enzyme treatment
43 mammalian cells
44 membrane
45 mice
46 non-viral carcinogens
47 normal cells
48 normal hamster cells
49 part
50 plant agglutinins
51 pronase
52 rats
53 receptor sites
54 receptors
55 results
56 saccharides
57 short incubation
58 sites
59 soybean agglutinin
60 such sites
61 surface membrane
62 transformation
63 treatment
64 trypsin
65 viral carcinogens
66 schema:name Different locations of carbohydrate-containing sites in the surface membrane of normal and transformed mammalian cells
67 schema:pagination 267-279
68 schema:productId N6a98fabe2c9d407f90384f199e73b160
69 Nbb1d844a89bf4826a0354e5d94f7ea52
70 Nfcaa187ef9e44fe38b4aea4392ba0f23
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046508734
72 https://doi.org/10.1007/bf01868019
73 schema:sdDatePublished 2022-01-01T18:00
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Nc52707ac776247bd9842500336bcb251
76 schema:url https://doi.org/10.1007/bf01868019
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N061ec00c9b2543fcbdb3d933ccd06e1f rdf:first sg:person.0614161163.93
81 rdf:rest N77e365b7ff08496b91be95cc60d39e87
82 N6a98fabe2c9d407f90384f199e73b160 schema:name pubmed_id
83 schema:value 24174197
84 rdf:type schema:PropertyValue
85 N77e365b7ff08496b91be95cc60d39e87 rdf:first sg:person.013464700163.25
86 rdf:rest Na3b8e6edf5494e27864d0a9a739f5aaf
87 N8dd09d3f0cd0461d9d5b31dacc8d45ed schema:issueNumber 1
88 rdf:type schema:PublicationIssue
89 Na3b8e6edf5494e27864d0a9a739f5aaf rdf:first sg:person.01315713223.72
90 rdf:rest Nb438b91d3d52431a964b68c9f75b5ba1
91 Nb438b91d3d52431a964b68c9f75b5ba1 rdf:first sg:person.047342437.65
92 rdf:rest rdf:nil
93 Nbb1d844a89bf4826a0354e5d94f7ea52 schema:name doi
94 schema:value 10.1007/bf01868019
95 rdf:type schema:PropertyValue
96 Nc52707ac776247bd9842500336bcb251 schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 Nefe60a9549b648d9af90d8487724acc8 schema:volumeNumber 3
99 rdf:type schema:PublicationVolume
100 Nfcaa187ef9e44fe38b4aea4392ba0f23 schema:name dimensions_id
101 schema:value pub.1046508734
102 rdf:type schema:PropertyValue
103 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
104 schema:name Biological Sciences
105 rdf:type schema:DefinedTerm
106 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
107 schema:name Biochemistry and Cell Biology
108 rdf:type schema:DefinedTerm
109 sg:journal.1012006 schema:issn 0022-2631
110 1432-1424
111 schema:name The Journal of Membrane Biology
112 schema:publisher Springer Nature
113 rdf:type schema:Periodical
114 sg:person.01315713223.72 schema:affiliation grid-institutes:grid.13992.30
115 schema:familyName Sharon
116 schema:givenName Nathan
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315713223.72
118 rdf:type schema:Person
119 sg:person.013464700163.25 schema:affiliation grid-institutes:grid.13992.30
120 schema:familyName Lis
121 schema:givenName Halina
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013464700163.25
123 rdf:type schema:Person
124 sg:person.047342437.65 schema:affiliation grid-institutes:grid.13992.30
125 schema:familyName Sachs
126 schema:givenName Leo
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047342437.65
128 rdf:type schema:Person
129 sg:person.0614161163.93 schema:affiliation grid-institutes:grid.13992.30
130 schema:familyName Sela
131 schema:givenName Ben-Ami
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614161163.93
133 rdf:type schema:Person
134 sg:pub.10.1038/2071272a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020383449
135 https://doi.org/10.1038/2071272a0
136 rdf:type schema:CreativeWork
137 sg:pub.10.1038/223710a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000989241
138 https://doi.org/10.1038/223710a0
139 rdf:type schema:CreativeWork
140 grid-institutes:grid.13992.30 schema:alternateName Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel
141 schema:name Departments of Genetics and Biophysics, Weizmann Institute of Science, Rehovoth, Israel
142 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...