Ontology type: schema:ScholarlyArticle
1992-08
AUTHORSThomas Wilk, Heike Mierswa, Hans-Georg Kräusslich, John J. Dunn, Valerie Bosch
ABSTRACTBacteriophage T7 RNA polymerase and a derivative containing a nuclear localization signal were transiently expressed in CV-1 cells and were shown to localize to the cytoplasm and nucleus, respectively. A vector was constructed containing T7 promoter and transcription terminator sequences flanking a picornaviral 5' untranslated sequence for cap-independent translation and a polyA signal. Expression of the HIV-1 envelope glycoproteins in this vector system gave high levels of specific transcripts and translation products, independent of the subcellular localization of T7 RNA polymerase. The synthesis of HIV glycoproteins was also completely independent of the coexpression of the HIV rev protein, which is normally required for the expression of HIV structural proteins. In addition, a polyA signal was not required, whereas the presence of the picornaviral 5' untranslated region was necessary for efficient expression. Different possibilities to account for these findings are discussed. The HIV glycoproteins synthesized in this system were normally processed and assembled; they could induce syncytium formation and complement an env-deletion mutant of HIV-1. More... »
PAGES229-246
http://scigraph.springernature.com/pub.10.1007/bf01702562
DOIhttp://dx.doi.org/10.1007/bf01702562
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1024234612
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/1413540
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bacteriophage T7",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA-Directed RNA Polymerases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, env",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, rev",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Vectors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glycoproteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HIV-1",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Messenger",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Fusion Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Viral Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "German Cancer Research Center",
"id": "https://www.grid.ac/institutes/grid.7497.d",
"name": [
"Institut f\u00fcr Virusforschung, Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, D-6900, Heidelberg, Germany"
],
"type": "Organization"
},
"familyName": "Wilk",
"givenName": "Thomas",
"id": "sg:person.01315142670.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315142670.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "German Cancer Research Center",
"id": "https://www.grid.ac/institutes/grid.7497.d",
"name": [
"Institut f\u00fcr Virusforschung, Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, D-6900, Heidelberg, Germany"
],
"type": "Organization"
},
"familyName": "Mierswa",
"givenName": "Heike",
"type": "Person"
},
{
"affiliation": {
"alternateName": "German Cancer Research Center",
"id": "https://www.grid.ac/institutes/grid.7497.d",
"name": [
"Institut f\u00fcr Virusforschung, Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, D-6900, Heidelberg, Germany"
],
"type": "Organization"
},
"familyName": "Kr\u00e4usslich",
"givenName": "Hans-Georg",
"id": "sg:person.0731120455.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731120455.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Brookhaven National Laboratory",
"id": "https://www.grid.ac/institutes/grid.202665.5",
"name": [
"Department of Biology, Brookhaven National Laboratory, Upton, NY, USA"
],
"type": "Organization"
},
"familyName": "Dunn",
"givenName": "John J.",
"id": "sg:person.013622502502.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013622502502.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "German Cancer Research Center",
"id": "https://www.grid.ac/institutes/grid.7497.d",
"name": [
"Institut f\u00fcr Virusforschung, Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, D-6900, Heidelberg, Germany"
],
"type": "Organization"
},
"familyName": "Bosch",
"givenName": "Valerie",
"id": "sg:person.0763527704.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763527704.43"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/228227a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001641086",
"https://doi.org/10.1038/228227a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/338254a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005345511",
"https://doi.org/10.1038/338254a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/338254a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005345511",
"https://doi.org/10.1038/338254a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.86.14.5400",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007124836"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/16.24.11840",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013070022"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(87)90021-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013321498"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/18.8.2037",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014178948"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/17.21.8485",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016554269"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2836(89)90483-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023310400"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.10.9.4529",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031242699"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(87)90171-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031905385"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(87)90165-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033703436"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(87)90165-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033703436"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.86.5.1495",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033842710"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.10.1.353",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037543751"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.83.21.8122",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040311251"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.7.7.2538",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043524487"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(88)90028-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045217125"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(88)90028-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045217125"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.86.16.6126",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049449394"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-2836(83)80282-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1054589670"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/dna.1.1984.3.479",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059250586"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2992081",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062580028"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078434029",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078663263",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079000239",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079419104",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079508450",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079511172",
"type": "CreativeWork"
}
],
"datePublished": "1992-08",
"datePublishedReg": "1992-08-01",
"description": "Bacteriophage T7 RNA polymerase and a derivative containing a nuclear localization signal were transiently expressed in CV-1 cells and were shown to localize to the cytoplasm and nucleus, respectively. A vector was constructed containing T7 promoter and transcription terminator sequences flanking a picornaviral 5' untranslated sequence for cap-independent translation and a polyA signal. Expression of the HIV-1 envelope glycoproteins in this vector system gave high levels of specific transcripts and translation products, independent of the subcellular localization of T7 RNA polymerase. The synthesis of HIV glycoproteins was also completely independent of the coexpression of the HIV rev protein, which is normally required for the expression of HIV structural proteins. In addition, a polyA signal was not required, whereas the presence of the picornaviral 5' untranslated region was necessary for efficient expression. Different possibilities to account for these findings are discussed. The HIV glycoproteins synthesized in this system were normally processed and assembled; they could induce syncytium formation and complement an env-deletion mutant of HIV-1.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf01702562",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1097920",
"issn": [
"0920-8569",
"1572-994X"
],
"name": "Virus Genes",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "6"
}
],
"name": "Expression of biologically active HIV glycoproteins using a T7 RNA polymerase-based eucaryotic vector system",
"pagination": "229-246",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"7374ee21187859ba35e63eb0adbe0de393fe2c1fcc5dd55bc378caaef14dcc52"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"1413540"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8803967"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf01702562"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1024234612"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf01702562",
"https://app.dimensions.ai/details/publication/pub.1024234612"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:35",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46775_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2FBF01702562"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01702562'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01702562'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01702562'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01702562'
This table displays all metadata directly associated to this object as RDF triples.
229 TRIPLES
21 PREDICATES
69 URIs
35 LITERALS
23 BLANK NODES