Problems and approaches in covalent attachment of peptides and proteins to inorganic surfaces for biosensor applications View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-05

AUTHORS

James E. Zull, J. Reed-Mundell, Y. W. Lee, Dmitri Vezenov, Nicholas P. Ziats, James M. Anderson, Chaim N. Sukenik

ABSTRACT

Some of the fundamental problems in covalent attachment of peptides and proteins to putative biosensor surfaces are reviewed and specific approaches to these problems discussed. In addition, selected aspects of our recent work utilizing self-assembled monolayer (SAM) systems designed to react selectively with the thiol side chain of Cys in proteins are presented. Uniform attachment of a 21-amino acid peptide antigen through a single Cys residue with retention of biological function (antibody binding) has been attained. Further work with this system may lead to solutions for some of the problems which currently prevent the development of reliable biosensors for industrial and medical use. More... »

PAGES

137-143

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01583997

DOI

http://dx.doi.org/10.1007/bf01583997

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048553556

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7764841


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