Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-05

AUTHORS

Maria de F. S. Barbosa, L. O. Ingram

ABSTRACT

The genes encodingZymomonas mobilis pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were expressed inBacillus subtilis YB886(pLOI500) under the control of aBacillus SPO2 phage promoter and caused a 50% reduction of growth rate compared with the unmodified vector. Expression was further confirmed by Western blots, activity stains of native gels, and in vitro measurements of alcohol dehydrogenase activity. Additional strains ofBacillus were also transformed, and all produced similar but low levels of these enzymes. Although higher specific activities will be required for efficient ethanol production, no fundamental barriers exist to the expression of theseZ. mobilis genes inBacillus. Two abundant new proteins (ca. mass 33,000 daltons and 14,000 daltons) were observed in Coomassie Blue-stained gels; they are similar in size to the proteins induced by recombinatn products inEscherichia coli. More... »

PAGES

279-282

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01573206

DOI

http://dx.doi.org/10.1007/bf01573206

DIMENSIONS

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