HLA antigen expression at the single cell level on a K562 × B cell hybrid: An analysis with monoclonal antibodies ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-11

AUTHORS

Andreas Ziegler, Barbara Uchańska-Ziegler, Jesper Zeuthen, Peter Wernet

ABSTRACT

The influence of different genetic environments on the expression of HLA complex-controlled antigens has been investigated using cell lines with various defects in the synthesis of these molecules and a somatic cell hybrid derived from them. A very sensitive bacterial binding assay allowing simultaneous evaluation of the morphology of a given cell and the quantity of a surface molecule has been developed for these studies. The fetal erythroid cell line K562, the Burkitt's lymphoma-derived cell line DAUDI, and their hybrid DUTKO1 have been employed. K562 and the hybrid, but not DAUDI, expressed HLA-A,B,C heavy chains as detected by the monoclonal antibody W6/32.HL, while two monoclonal antibodies (TU48 and 2BC4) against the supertypic specificities HLA-Bw4 and Bw6 showed no reactivity. The presence of human Ia-like antigens on the cell surfaces was investigated with a panel of eight monoclonal antibodies. K562 cells were completely unreactive, and DAUDI cells gave the expected positive reaction, but about 1% or less of the cells in the DUTKO1 population appeared to express these antigens as well. We discuss possible reasons for the failure to detect HLA-B antigens with monoclonal antibodies and the lack of complete "dominance" of the K562 genome in the hybrid cell line. More... »

PAGES

775-789

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01543018

DOI

http://dx.doi.org/10.1007/bf01543018

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015211415

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6187075


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

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N-Triples is a line-based linked data format ideal for batch operations.

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Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01543018'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01543018'


 

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