Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-09

AUTHORS

L. A. Chasin, G. Urlaub

ABSTRACT

Chinese hamster ovary cell mutants resistant to the purine analogs 6-thioguanine or 8-azaguanine have been isolated following mutagenesis with ethyl methane sulfonate. The activities of hypoxanthine phosphoribosyltransferase (HPRT) in three such mutants have been found to exhibit an increased Km for the substrate 5-phosphoribosyl-1-pyrophosphate. The isoelectric point of the mutant enzyme activity has also changed in two mutants. Hybrid cells containing one mutant and one wild-type allele express both genes. Segregants that have lost only the wild-type allele can be selected on the basis of drug resistance. Two mutants exhibiting different alterations in HPRT activity can complement in a hybrid cell to yield a wild-type growth pattern and enzyme activity with intermediate electrophoretic and kinetic properties. The results suggest intracistronic complementation between structural gene mutants of HPRT. More... »

PAGES

453-467

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01542725

DOI

http://dx.doi.org/10.1007/bf01542725

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053584880

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1027153


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alleles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Dominant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Complementation Test", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hybrid Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hypoxanthine Phosphoribosyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoribosyl Pyrophosphate", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Columbia University", 
          "id": "https://www.grid.ac/institutes/grid.21729.3f", 
          "name": [
            "Department of Biological Sciences, Columbia University, 10027, New York, New York"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chasin", 
        "givenName": "L. A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Columbia University", 
          "id": "https://www.grid.ac/institutes/grid.21729.3f", 
          "name": [
            "Department of Biological Sciences, Columbia University, 10027, New York, New York"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Urlaub", 
        "givenName": "G.", 
        "id": "sg:person.0737755763.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737755763.39"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/newbio240050a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000515668", 
          "https://doi.org/10.1038/newbio240050a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio240050a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000515668", 
          "https://doi.org/10.1038/newbio240050a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio240050a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000515668", 
          "https://doi.org/10.1038/newbio240050a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.08.120174.001211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004243518"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040780113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004341046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.71.12.4732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005925586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040860413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006245620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(72)90446-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007734009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.72.2.493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007890395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040820218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009509502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00485733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009520456", 
          "https://doi.org/10.1007/bf00485733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00485733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009520456", 
          "https://doi.org/10.1007/bf00485733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.103.2.273", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010217013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(74)90006-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012741937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040850217", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014030239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(73)90593-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014848579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00485734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014853793", 
          "https://doi.org/10.1007/bf00485734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00485734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014853793", 
          "https://doi.org/10.1007/bf00485734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(76)90249-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016130388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.60.4.1275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019156295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040810112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019253055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.71.5.2057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020181724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(75)90123-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020680771"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.72.1.98", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020866114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.53.2.288", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028325367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(70)90100-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030536764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(75)90002-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032086971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.62.4.1085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036659259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-185x.1972.tb00969.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036794824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01538731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037880647", 
          "https://doi.org/10.1007/bf01538731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01538731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037880647", 
          "https://doi.org/10.1007/bf01538731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2944(69)90022-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038709254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.03.120169.002431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044356998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.68.1.116", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044735807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.09.120175.002203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044860330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.70.9.2590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045488286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00487447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045488979", 
          "https://doi.org/10.1007/bf00487447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00487447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045488979", 
          "https://doi.org/10.1007/bf00487447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01538451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046342936", 
          "https://doi.org/10.1007/bf01538451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01538451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046342936", 
          "https://doi.org/10.1007/bf01538451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.70.11.3145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046379133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.64.4.1284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048937258"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(74)90261-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052467487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00487007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053436020", 
          "https://doi.org/10.1007/bf00487007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00487007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053436020", 
          "https://doi.org/10.1007/bf00487007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00865a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055191614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.175.4029.1467", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062504605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074944607", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074952594", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076750128", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080301392", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080454833", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080827391", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1976-09", 
    "datePublishedReg": "1976-09-01", 
    "description": "Chinese hamster ovary cell mutants resistant to the purine analogs 6-thioguanine or 8-azaguanine have been isolated following mutagenesis with ethyl methane sulfonate. The activities of hypoxanthine phosphoribosyltransferase (HPRT) in three such mutants have been found to exhibit an increased Km for the substrate 5-phosphoribosyl-1-pyrophosphate. The isoelectric point of the mutant enzyme activity has also changed in two mutants. Hybrid cells containing one mutant and one wild-type allele express both genes. Segregants that have lost only the wild-type allele can be selected on the basis of drug resistance. Two mutants exhibiting different alterations in HPRT activity can complement in a hybrid cell to yield a wild-type growth pattern and enzyme activity with intermediate electrophoretic and kinetic properties. The results suggest intracistronic complementation between structural gene mutants of HPRT.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf01542725", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083126", 
        "issn": [
          "0098-0366"
        ], 
        "name": "Somatic Cell Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "2"
      }
    ], 
    "name": "Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cells", 
    "pagination": "453-467", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ff3b6d312ebefe3cccc8eaa105d68d53394c57b4cce518835b815965e1200c5c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1027153"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7506054"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01542725"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1053584880"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01542725", 
      "https://app.dimensions.ai/details/publication/pub.1053584880"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46777_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF01542725"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01542725'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01542725'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01542725'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01542725'


 

This table displays all metadata directly associated to this object as RDF triples.

266 TRIPLES      21 PREDICATES      88 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01542725 schema:about N12c3aca5ebe746bc8bf2629590ba0412
2 N162dcbb46b0b4d4b8bda2c6b6ca05df8
3 N5662363f22514a73865124f4e6c956f1
4 N575ed538be7c4a8e86a9a69d6f37f789
5 N5f10203150a940c79bc592b830980f00
6 N66ff4c9a04854dc5bf0fc26fe019832a
7 Na4562964f37d486c83016c1b1bf41986
8 Naafdf343ad9c4a8f9dd3eb8913e3063b
9 Nca10ed62911d47cfb0023c642c2dd98d
10 Nd111d63f74704b5591193eb438ff2d69
11 Nd25c4b1517a84e6486b52cc23ae22e7f
12 Nd5906c301b5e410fad26ac0cd962f8a0
13 Ne9ae2db3e54f46f38e8d1cdba854a610
14 Nfd8b4c91c47342249239fc11c3183988
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N25bed8ad18684f8db5caff07e2a4c64c
18 schema:citation sg:pub.10.1007/bf00485733
19 sg:pub.10.1007/bf00485734
20 sg:pub.10.1007/bf00487007
21 sg:pub.10.1007/bf00487447
22 sg:pub.10.1007/bf01538451
23 sg:pub.10.1007/bf01538731
24 sg:pub.10.1038/newbio240050a0
25 https://app.dimensions.ai/details/publication/pub.1074944607
26 https://app.dimensions.ai/details/publication/pub.1074952594
27 https://app.dimensions.ai/details/publication/pub.1076750128
28 https://app.dimensions.ai/details/publication/pub.1080301392
29 https://app.dimensions.ai/details/publication/pub.1080454833
30 https://app.dimensions.ai/details/publication/pub.1080827391
31 https://doi.org/10.1002/jcp.1040780113
32 https://doi.org/10.1002/jcp.1040810112
33 https://doi.org/10.1002/jcp.1040820218
34 https://doi.org/10.1002/jcp.1040850217
35 https://doi.org/10.1002/jcp.1040860413
36 https://doi.org/10.1016/0003-2697(70)90100-4
37 https://doi.org/10.1016/0006-2944(69)90022-2
38 https://doi.org/10.1016/0014-4827(72)90446-6
39 https://doi.org/10.1016/0014-4827(73)90593-4
40 https://doi.org/10.1016/0014-4827(74)90261-4
41 https://doi.org/10.1016/0092-8674(74)90006-3
42 https://doi.org/10.1016/0092-8674(75)90002-1
43 https://doi.org/10.1016/0092-8674(75)90123-3
44 https://doi.org/10.1016/0092-8674(76)90249-x
45 https://doi.org/10.1021/bi00865a004
46 https://doi.org/10.1073/pnas.53.2.288
47 https://doi.org/10.1073/pnas.60.4.1275
48 https://doi.org/10.1073/pnas.62.4.1085
49 https://doi.org/10.1073/pnas.64.4.1284
50 https://doi.org/10.1073/pnas.68.1.116
51 https://doi.org/10.1073/pnas.70.11.3145
52 https://doi.org/10.1073/pnas.70.9.2590
53 https://doi.org/10.1073/pnas.71.12.4732
54 https://doi.org/10.1073/pnas.71.5.2057
55 https://doi.org/10.1073/pnas.72.1.98
56 https://doi.org/10.1073/pnas.72.2.493
57 https://doi.org/10.1084/jem.103.2.273
58 https://doi.org/10.1111/j.1469-185x.1972.tb00969.x
59 https://doi.org/10.1126/science.175.4029.1467
60 https://doi.org/10.1146/annurev.ge.03.120169.002431
61 https://doi.org/10.1146/annurev.ge.08.120174.001211
62 https://doi.org/10.1146/annurev.ge.09.120175.002203
63 schema:datePublished 1976-09
64 schema:datePublishedReg 1976-09-01
65 schema:description Chinese hamster ovary cell mutants resistant to the purine analogs 6-thioguanine or 8-azaguanine have been isolated following mutagenesis with ethyl methane sulfonate. The activities of hypoxanthine phosphoribosyltransferase (HPRT) in three such mutants have been found to exhibit an increased Km for the substrate 5-phosphoribosyl-1-pyrophosphate. The isoelectric point of the mutant enzyme activity has also changed in two mutants. Hybrid cells containing one mutant and one wild-type allele express both genes. Segregants that have lost only the wild-type allele can be selected on the basis of drug resistance. Two mutants exhibiting different alterations in HPRT activity can complement in a hybrid cell to yield a wild-type growth pattern and enzyme activity with intermediate electrophoretic and kinetic properties. The results suggest intracistronic complementation between structural gene mutants of HPRT.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree false
69 schema:isPartOf N031cebe5a01e42b19ea7301de8260182
70 N1ff50e2f774647a6a583746470839461
71 sg:journal.1083126
72 schema:name Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cells
73 schema:pagination 453-467
74 schema:productId N230c453bb08c41d6a56fc88cac68f353
75 N31f155be090f4dc9bef715530b5bf8a3
76 N4a47b7ef8a984b9eb4b7a0cabae86bb0
77 Nf6f8fbde5069493189887958bba8635b
78 Nf9a487f943b944bfadb3d4d5bee56238
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053584880
80 https://doi.org/10.1007/bf01542725
81 schema:sdDatePublished 2019-04-11T13:36
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher Nd8f26f7da90240d097776924c826a762
84 schema:url http://link.springer.com/10.1007/BF01542725
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N031cebe5a01e42b19ea7301de8260182 schema:issueNumber 5
89 rdf:type schema:PublicationIssue
90 N12c3aca5ebe746bc8bf2629590ba0412 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Drug Resistance
92 rdf:type schema:DefinedTerm
93 N162dcbb46b0b4d4b8bda2c6b6ca05df8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Animals
95 rdf:type schema:DefinedTerm
96 N166fe77de33546589b030e487c4ca4fc schema:affiliation https://www.grid.ac/institutes/grid.21729.3f
97 schema:familyName Chasin
98 schema:givenName L. A.
99 rdf:type schema:Person
100 N1ff50e2f774647a6a583746470839461 schema:volumeNumber 2
101 rdf:type schema:PublicationVolume
102 N230c453bb08c41d6a56fc88cac68f353 schema:name dimensions_id
103 schema:value pub.1053584880
104 rdf:type schema:PropertyValue
105 N25bed8ad18684f8db5caff07e2a4c64c rdf:first N166fe77de33546589b030e487c4ca4fc
106 rdf:rest Nf356ccd93f0f4772896f5b552036eacf
107 N31f155be090f4dc9bef715530b5bf8a3 schema:name nlm_unique_id
108 schema:value 7506054
109 rdf:type schema:PropertyValue
110 N4a47b7ef8a984b9eb4b7a0cabae86bb0 schema:name pubmed_id
111 schema:value 1027153
112 rdf:type schema:PropertyValue
113 N5662363f22514a73865124f4e6c956f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Mutation
115 rdf:type schema:DefinedTerm
116 N575ed538be7c4a8e86a9a69d6f37f789 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Cricetinae
118 rdf:type schema:DefinedTerm
119 N5f10203150a940c79bc592b830980f00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Phenotype
121 rdf:type schema:DefinedTerm
122 N66ff4c9a04854dc5bf0fc26fe019832a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Phosphoribosyl Pyrophosphate
124 rdf:type schema:DefinedTerm
125 Na4562964f37d486c83016c1b1bf41986 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Genetic Complementation Test
127 rdf:type schema:DefinedTerm
128 Naafdf343ad9c4a8f9dd3eb8913e3063b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Alleles
130 rdf:type schema:DefinedTerm
131 Nca10ed62911d47cfb0023c642c2dd98d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Hypoxanthine Phosphoribosyltransferase
133 rdf:type schema:DefinedTerm
134 Nd111d63f74704b5591193eb438ff2d69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Genes, Dominant
136 rdf:type schema:DefinedTerm
137 Nd25c4b1517a84e6486b52cc23ae22e7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Hybrid Cells
139 rdf:type schema:DefinedTerm
140 Nd5906c301b5e410fad26ac0cd962f8a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Chromosome Mapping
142 rdf:type schema:DefinedTerm
143 Nd8f26f7da90240d097776924c826a762 schema:name Springer Nature - SN SciGraph project
144 rdf:type schema:Organization
145 Ne9ae2db3e54f46f38e8d1cdba854a610 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name In Vitro Techniques
147 rdf:type schema:DefinedTerm
148 Nf356ccd93f0f4772896f5b552036eacf rdf:first sg:person.0737755763.39
149 rdf:rest rdf:nil
150 Nf6f8fbde5069493189887958bba8635b schema:name readcube_id
151 schema:value ff3b6d312ebefe3cccc8eaa105d68d53394c57b4cce518835b815965e1200c5c
152 rdf:type schema:PropertyValue
153 Nf9a487f943b944bfadb3d4d5bee56238 schema:name doi
154 schema:value 10.1007/bf01542725
155 rdf:type schema:PropertyValue
156 Nfd8b4c91c47342249239fc11c3183988 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Genes
158 rdf:type schema:DefinedTerm
159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biological Sciences
161 rdf:type schema:DefinedTerm
162 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biochemistry and Cell Biology
164 rdf:type schema:DefinedTerm
165 sg:journal.1083126 schema:issn 0098-0366
166 schema:name Somatic Cell Genetics
167 rdf:type schema:Periodical
168 sg:person.0737755763.39 schema:affiliation https://www.grid.ac/institutes/grid.21729.3f
169 schema:familyName Urlaub
170 schema:givenName G.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737755763.39
172 rdf:type schema:Person
173 sg:pub.10.1007/bf00485733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009520456
174 https://doi.org/10.1007/bf00485733
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/bf00485734 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014853793
177 https://doi.org/10.1007/bf00485734
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/bf00487007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053436020
180 https://doi.org/10.1007/bf00487007
181 rdf:type schema:CreativeWork
182 sg:pub.10.1007/bf00487447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045488979
183 https://doi.org/10.1007/bf00487447
184 rdf:type schema:CreativeWork
185 sg:pub.10.1007/bf01538451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046342936
186 https://doi.org/10.1007/bf01538451
187 rdf:type schema:CreativeWork
188 sg:pub.10.1007/bf01538731 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037880647
189 https://doi.org/10.1007/bf01538731
190 rdf:type schema:CreativeWork
191 sg:pub.10.1038/newbio240050a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000515668
192 https://doi.org/10.1038/newbio240050a0
193 rdf:type schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1074944607 schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1074952594 schema:CreativeWork
196 https://app.dimensions.ai/details/publication/pub.1076750128 schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1080301392 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1080454833 schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1080827391 schema:CreativeWork
200 https://doi.org/10.1002/jcp.1040780113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004341046
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1002/jcp.1040810112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019253055
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1002/jcp.1040820218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009509502
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1002/jcp.1040850217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014030239
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1002/jcp.1040860413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006245620
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0003-2697(70)90100-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030536764
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0006-2944(69)90022-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038709254
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0014-4827(72)90446-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007734009
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0014-4827(73)90593-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014848579
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0014-4827(74)90261-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052467487
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0092-8674(74)90006-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012741937
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/0092-8674(75)90002-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032086971
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0092-8674(75)90123-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020680771
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/0092-8674(76)90249-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016130388
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1021/bi00865a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055191614
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.53.2.288 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028325367
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1073/pnas.60.4.1275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019156295
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1073/pnas.62.4.1085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036659259
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.64.4.1284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048937258
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1073/pnas.68.1.116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044735807
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1073/pnas.70.11.3145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046379133
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.70.9.2590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045488286
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.71.12.4732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005925586
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.71.5.2057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020181724
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.72.1.98 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020866114
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1073/pnas.72.2.493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007890395
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1084/jem.103.2.273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010217013
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1111/j.1469-185x.1972.tb00969.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036794824
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1126/science.175.4029.1467 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062504605
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1146/annurev.ge.03.120169.002431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044356998
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1146/annurev.ge.08.120174.001211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004243518
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1146/annurev.ge.09.120175.002203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044860330
263 rdf:type schema:CreativeWork
264 https://www.grid.ac/institutes/grid.21729.3f schema:alternateName Columbia University
265 schema:name Department of Biological Sciences, Columbia University, 10027, New York, New York
266 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...