Supermelanotic hybrids derived from mouse melanomas and normal mouse cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-01

AUTHORS

R. Halaban, J. Nordlund, U. Francke, G. Moellmann, J. M. Eisenstadt

ABSTRACT

Hybrids formed between HPRT− Cloudman mouse melanoma and normal cells were isolated. The parental origin of the hybrids was verified by isozyme and karyotype analyses. These hybrid cells differed in two major characteristics from hybrids of melanoma and established fibroblastic cells. (1) They grew as tumors when injected into mice, and (2) they expressed differentiated melanocytic functions. At least one of the differentiated functions was overexpressed. The specific activity of tyrosinase was 3–20 times higher in the hybrid cells than in the parental mouse melanoma. The overexpression of tyrosinase in these hybrid cells has been stable for more than a year, has been transmitted to subclones of the original hybrid cell lines, and has been expressed in tumors that grew after injections of hybrid cells into animals. More... »

PAGES

29-44

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01538694

DOI

http://dx.doi.org/10.1007/bf01538694

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003711667

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6768142


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